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Glutathione S-transferases play a role in the detoxification of flumethrin and chlorpyrifos in Haemaphysalis longicornis

BACKGROUND: Haemaphysalis longicornis is a tick of importance to health, as it serves as a vector of several pathogens, including Theileria orientalis, Babesia ovata, Rickettsia japonica and the severe fever with thrombocytopenia syndrome virus (SFTSV). Presently, the major method of control for thi...

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Autores principales: Hernandez, Emmanuel Pacia, Kusakisako, Kodai, Talactac, Melbourne Rio, Galay, Remil Linggatong, Hatta, Takeshi, Fujisaki, Kozo, Tsuji, Naotoshi, Tanaka, Tetsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6085608/
https://www.ncbi.nlm.nih.gov/pubmed/30092823
http://dx.doi.org/10.1186/s13071-018-3044-9
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author Hernandez, Emmanuel Pacia
Kusakisako, Kodai
Talactac, Melbourne Rio
Galay, Remil Linggatong
Hatta, Takeshi
Fujisaki, Kozo
Tsuji, Naotoshi
Tanaka, Tetsuya
author_facet Hernandez, Emmanuel Pacia
Kusakisako, Kodai
Talactac, Melbourne Rio
Galay, Remil Linggatong
Hatta, Takeshi
Fujisaki, Kozo
Tsuji, Naotoshi
Tanaka, Tetsuya
author_sort Hernandez, Emmanuel Pacia
collection PubMed
description BACKGROUND: Haemaphysalis longicornis is a tick of importance to health, as it serves as a vector of several pathogens, including Theileria orientalis, Babesia ovata, Rickettsia japonica and the severe fever with thrombocytopenia syndrome virus (SFTSV). Presently, the major method of control for this tick is the use of chemical acaricides. The glutathione S-transferase (GST) system is one mechanism through which the tick metabolizes these acaricides. Two GSTs from H. longicornis (HlGST and HlGST2) have been previously identified. RESULTS: Enzyme kinetic studies were performed to determine the interaction of acaricides with recombinant H. longicornis GSTs. Recombinant HlGST activity was inhibited by flumethrin and cypermethrin, while recombinant HlGST2 activity was inhibited by chlorpyrifos and cypermethrin. Using real-time RT-PCR, the upregulation of the HlGST gene was observed upon exposure to sublethal doses of flumethrin, while the HlGST2 gene was upregulated when exposed to sublethal doses of chlorpyrifos. Sex and strain dependencies in the induction of GST gene expression by flumethrin were also observed. Knockdown of the HlGST gene resulted in the increased susceptibility of larvae and adult male ticks to sublethal doses of flumethrin and the susceptibility of larvae against sublethal doses of chlorpyrifos was increased upon knockdown of HlGST2. CONCLUSIONS: HlGST could be vital for the metabolism of flumethrin in larvae and adult male ticks, while HlGST2 is important in the detoxification of chlorpyrifos in larval ticks. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-018-3044-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-60856082018-08-16 Glutathione S-transferases play a role in the detoxification of flumethrin and chlorpyrifos in Haemaphysalis longicornis Hernandez, Emmanuel Pacia Kusakisako, Kodai Talactac, Melbourne Rio Galay, Remil Linggatong Hatta, Takeshi Fujisaki, Kozo Tsuji, Naotoshi Tanaka, Tetsuya Parasit Vectors Research BACKGROUND: Haemaphysalis longicornis is a tick of importance to health, as it serves as a vector of several pathogens, including Theileria orientalis, Babesia ovata, Rickettsia japonica and the severe fever with thrombocytopenia syndrome virus (SFTSV). Presently, the major method of control for this tick is the use of chemical acaricides. The glutathione S-transferase (GST) system is one mechanism through which the tick metabolizes these acaricides. Two GSTs from H. longicornis (HlGST and HlGST2) have been previously identified. RESULTS: Enzyme kinetic studies were performed to determine the interaction of acaricides with recombinant H. longicornis GSTs. Recombinant HlGST activity was inhibited by flumethrin and cypermethrin, while recombinant HlGST2 activity was inhibited by chlorpyrifos and cypermethrin. Using real-time RT-PCR, the upregulation of the HlGST gene was observed upon exposure to sublethal doses of flumethrin, while the HlGST2 gene was upregulated when exposed to sublethal doses of chlorpyrifos. Sex and strain dependencies in the induction of GST gene expression by flumethrin were also observed. Knockdown of the HlGST gene resulted in the increased susceptibility of larvae and adult male ticks to sublethal doses of flumethrin and the susceptibility of larvae against sublethal doses of chlorpyrifos was increased upon knockdown of HlGST2. CONCLUSIONS: HlGST could be vital for the metabolism of flumethrin in larvae and adult male ticks, while HlGST2 is important in the detoxification of chlorpyrifos in larval ticks. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-018-3044-9) contains supplementary material, which is available to authorized users. BioMed Central 2018-08-09 /pmc/articles/PMC6085608/ /pubmed/30092823 http://dx.doi.org/10.1186/s13071-018-3044-9 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Hernandez, Emmanuel Pacia
Kusakisako, Kodai
Talactac, Melbourne Rio
Galay, Remil Linggatong
Hatta, Takeshi
Fujisaki, Kozo
Tsuji, Naotoshi
Tanaka, Tetsuya
Glutathione S-transferases play a role in the detoxification of flumethrin and chlorpyrifos in Haemaphysalis longicornis
title Glutathione S-transferases play a role in the detoxification of flumethrin and chlorpyrifos in Haemaphysalis longicornis
title_full Glutathione S-transferases play a role in the detoxification of flumethrin and chlorpyrifos in Haemaphysalis longicornis
title_fullStr Glutathione S-transferases play a role in the detoxification of flumethrin and chlorpyrifos in Haemaphysalis longicornis
title_full_unstemmed Glutathione S-transferases play a role in the detoxification of flumethrin and chlorpyrifos in Haemaphysalis longicornis
title_short Glutathione S-transferases play a role in the detoxification of flumethrin and chlorpyrifos in Haemaphysalis longicornis
title_sort glutathione s-transferases play a role in the detoxification of flumethrin and chlorpyrifos in haemaphysalis longicornis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6085608/
https://www.ncbi.nlm.nih.gov/pubmed/30092823
http://dx.doi.org/10.1186/s13071-018-3044-9
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