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The role of the Wnt/β-catenin signaling pathway in the proliferation of gold nanoparticle-treated human periodontal ligament stem cells
BACKGROUND: Several studies have confirmed that gold nanoparticles (AuNPs) of specific concentration and size exert a boosting effect on cell proliferation; however, the mechanism through which this effect occurs remains unknown. This study explores the canonical Wnt signaling pathway in AuNP promot...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6085621/ https://www.ncbi.nlm.nih.gov/pubmed/30092818 http://dx.doi.org/10.1186/s13287-018-0954-6 |
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author | Li, Chen Li, Zhuoquan Zhang, Yan Fathy, Abdel Hamid Zhou, Min |
author_facet | Li, Chen Li, Zhuoquan Zhang, Yan Fathy, Abdel Hamid Zhou, Min |
author_sort | Li, Chen |
collection | PubMed |
description | BACKGROUND: Several studies have confirmed that gold nanoparticles (AuNPs) of specific concentration and size exert a boosting effect on cell proliferation; however, the mechanism through which this effect occurs remains unknown. This study explores the canonical Wnt signaling pathway in AuNP promotion of human periodontal ligament stem cell (hPDLSC) proliferation. METHODS: MTS was employed to evaluate hPDLSC proliferation. The interference of LRP5 and β-catenin was steered by shRNA plasmids and siRNA, respectively, at which point the expression of MYC, CCND1, AXIN2, and POU5F1 had been estimated via real-time PCR. The expressions of LRP5 and β-catenin were detected via western blot assay. RESULTS: The proliferation of hPDLSCs treated with 60 nm AuNPs at 56 μM was clearly elevated. In contrast, β-catenin siRNA significantly decreased cell viability. The LRP5 shRNA plasmid did not consistently impact cells. The expressions of these four genes downstream of the Wnt/β-catenin signaling pathway were not significantly overexpressed in response to the interference of shRNA plasmid/siRNA with the treatment of AuNPs. CONCLUSIONS: These results suggest that the Wnt/β-catenin signaling pathway plays a significant role in the process of AuNP promotion of hPDLSC proliferation. |
format | Online Article Text |
id | pubmed-6085621 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-60856212018-08-16 The role of the Wnt/β-catenin signaling pathway in the proliferation of gold nanoparticle-treated human periodontal ligament stem cells Li, Chen Li, Zhuoquan Zhang, Yan Fathy, Abdel Hamid Zhou, Min Stem Cell Res Ther Research BACKGROUND: Several studies have confirmed that gold nanoparticles (AuNPs) of specific concentration and size exert a boosting effect on cell proliferation; however, the mechanism through which this effect occurs remains unknown. This study explores the canonical Wnt signaling pathway in AuNP promotion of human periodontal ligament stem cell (hPDLSC) proliferation. METHODS: MTS was employed to evaluate hPDLSC proliferation. The interference of LRP5 and β-catenin was steered by shRNA plasmids and siRNA, respectively, at which point the expression of MYC, CCND1, AXIN2, and POU5F1 had been estimated via real-time PCR. The expressions of LRP5 and β-catenin were detected via western blot assay. RESULTS: The proliferation of hPDLSCs treated with 60 nm AuNPs at 56 μM was clearly elevated. In contrast, β-catenin siRNA significantly decreased cell viability. The LRP5 shRNA plasmid did not consistently impact cells. The expressions of these four genes downstream of the Wnt/β-catenin signaling pathway were not significantly overexpressed in response to the interference of shRNA plasmid/siRNA with the treatment of AuNPs. CONCLUSIONS: These results suggest that the Wnt/β-catenin signaling pathway plays a significant role in the process of AuNP promotion of hPDLSC proliferation. BioMed Central 2018-08-09 /pmc/articles/PMC6085621/ /pubmed/30092818 http://dx.doi.org/10.1186/s13287-018-0954-6 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Li, Chen Li, Zhuoquan Zhang, Yan Fathy, Abdel Hamid Zhou, Min The role of the Wnt/β-catenin signaling pathway in the proliferation of gold nanoparticle-treated human periodontal ligament stem cells |
title | The role of the Wnt/β-catenin signaling pathway in the proliferation of gold nanoparticle-treated human periodontal ligament stem cells |
title_full | The role of the Wnt/β-catenin signaling pathway in the proliferation of gold nanoparticle-treated human periodontal ligament stem cells |
title_fullStr | The role of the Wnt/β-catenin signaling pathway in the proliferation of gold nanoparticle-treated human periodontal ligament stem cells |
title_full_unstemmed | The role of the Wnt/β-catenin signaling pathway in the proliferation of gold nanoparticle-treated human periodontal ligament stem cells |
title_short | The role of the Wnt/β-catenin signaling pathway in the proliferation of gold nanoparticle-treated human periodontal ligament stem cells |
title_sort | role of the wnt/β-catenin signaling pathway in the proliferation of gold nanoparticle-treated human periodontal ligament stem cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6085621/ https://www.ncbi.nlm.nih.gov/pubmed/30092818 http://dx.doi.org/10.1186/s13287-018-0954-6 |
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