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Dehiscence method: a seed-saving, quick and simple viability assessment in rice

BACKGROUND: Seed viability monitoring is very important in ex situ germplasm preservation to detect germplasm deterioration. This requires seed-, time- and labor- saving methods with high precision to assess seed germination as viability. Although the current non-invasive, rapid, sensing methods (NR...

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Autores principales: Xu, Ling-xiang, Lin, Yi-xin, Wang, Li-hong, Zhou, Yuan-chang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6085679/
https://www.ncbi.nlm.nih.gov/pubmed/30116291
http://dx.doi.org/10.1186/s13007-018-0334-3
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author Xu, Ling-xiang
Lin, Yi-xin
Wang, Li-hong
Zhou, Yuan-chang
author_facet Xu, Ling-xiang
Lin, Yi-xin
Wang, Li-hong
Zhou, Yuan-chang
author_sort Xu, Ling-xiang
collection PubMed
description BACKGROUND: Seed viability monitoring is very important in ex situ germplasm preservation to detect germplasm deterioration. This requires seed-, time- and labor- saving methods with high precision to assess seed germination as viability. Although the current non-invasive, rapid, sensing methods (NRSs) are time- and labor-saving, they lack the precision and simplicity which are the virtues of traditional germination. Moreover, they consume a considerable amount of seeds to adjust sensed signals to germination percentage, which disregards the seed-saving objective. This becomes particularly severe for rare or endangered species whose seeds are already scarce. Here we propose a new method that is precise, low-invasive, simple, and quick, which involves analyzing the pattern of dehiscence (seed coat rupture), followed by embryonic protrusion. RESULTS: Dehiscence proved simple to identify. After the trial of 20 treatments from 3 rice varieties, we recognized that dehiscence percentage at the 48th hour of germination (D(48)) correlates significantly with germination rate for tested seed lots. In addition, we found that the final germination percentage corresponded to D(48) plus 5. More than 70% of the seeds survived post-dehiscence desiccation for storage. Hydrogen peroxide (1 mM) as the solution for imbibition could further improve the survival. The method also worked quicker than tetrazolium which is honored as a fast, traditional method, in detecting less vigorous but viable seeds. CONCLUSION: We demonstrated the comprehensive virtues of dehiscence method in assessing rice seed: it is more precise and easier to use than NRSs and is faster and more seed-saving than traditional methods. We anticipate modifications including artificial intelligence to extend our method to increasingly diverse circumstances and species. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13007-018-0334-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-60856792018-08-16 Dehiscence method: a seed-saving, quick and simple viability assessment in rice Xu, Ling-xiang Lin, Yi-xin Wang, Li-hong Zhou, Yuan-chang Plant Methods Research BACKGROUND: Seed viability monitoring is very important in ex situ germplasm preservation to detect germplasm deterioration. This requires seed-, time- and labor- saving methods with high precision to assess seed germination as viability. Although the current non-invasive, rapid, sensing methods (NRSs) are time- and labor-saving, they lack the precision and simplicity which are the virtues of traditional germination. Moreover, they consume a considerable amount of seeds to adjust sensed signals to germination percentage, which disregards the seed-saving objective. This becomes particularly severe for rare or endangered species whose seeds are already scarce. Here we propose a new method that is precise, low-invasive, simple, and quick, which involves analyzing the pattern of dehiscence (seed coat rupture), followed by embryonic protrusion. RESULTS: Dehiscence proved simple to identify. After the trial of 20 treatments from 3 rice varieties, we recognized that dehiscence percentage at the 48th hour of germination (D(48)) correlates significantly with germination rate for tested seed lots. In addition, we found that the final germination percentage corresponded to D(48) plus 5. More than 70% of the seeds survived post-dehiscence desiccation for storage. Hydrogen peroxide (1 mM) as the solution for imbibition could further improve the survival. The method also worked quicker than tetrazolium which is honored as a fast, traditional method, in detecting less vigorous but viable seeds. CONCLUSION: We demonstrated the comprehensive virtues of dehiscence method in assessing rice seed: it is more precise and easier to use than NRSs and is faster and more seed-saving than traditional methods. We anticipate modifications including artificial intelligence to extend our method to increasingly diverse circumstances and species. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13007-018-0334-3) contains supplementary material, which is available to authorized users. BioMed Central 2018-08-10 /pmc/articles/PMC6085679/ /pubmed/30116291 http://dx.doi.org/10.1186/s13007-018-0334-3 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Xu, Ling-xiang
Lin, Yi-xin
Wang, Li-hong
Zhou, Yuan-chang
Dehiscence method: a seed-saving, quick and simple viability assessment in rice
title Dehiscence method: a seed-saving, quick and simple viability assessment in rice
title_full Dehiscence method: a seed-saving, quick and simple viability assessment in rice
title_fullStr Dehiscence method: a seed-saving, quick and simple viability assessment in rice
title_full_unstemmed Dehiscence method: a seed-saving, quick and simple viability assessment in rice
title_short Dehiscence method: a seed-saving, quick and simple viability assessment in rice
title_sort dehiscence method: a seed-saving, quick and simple viability assessment in rice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6085679/
https://www.ncbi.nlm.nih.gov/pubmed/30116291
http://dx.doi.org/10.1186/s13007-018-0334-3
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