Cargando…
Characterization of the glycerol-3-phosphate acyltransferase gene and its real-time expression under cold stress in Paeonia lactiflora Pall
Elucidating the cold tolerance mechanism of Paeonia lactiflora, which is one of the most valuable ornamental and medicinal plants in Asia, fundamentally impacts its breeding and production. The glycerol-3-phosphate acyltransferase (GPAT) gene plays a pivotal role in cold resistance in a variety of p...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6086452/ https://www.ncbi.nlm.nih.gov/pubmed/30096187 http://dx.doi.org/10.1371/journal.pone.0202168 |
_version_ | 1783346517415821312 |
---|---|
author | Li, Xueting Liu, Ping Yang, Panpan Fan, Chuanzhu Sun, Xiaomei |
author_facet | Li, Xueting Liu, Ping Yang, Panpan Fan, Chuanzhu Sun, Xiaomei |
author_sort | Li, Xueting |
collection | PubMed |
description | Elucidating the cold tolerance mechanism of Paeonia lactiflora, which is one of the most valuable ornamental and medicinal plants in Asia, fundamentally impacts its breeding and production. The glycerol-3-phosphate acyltransferase (GPAT) gene plays a pivotal role in cold resistance in a variety of plant species. Here, we cloned the P. lactiflora GPAT gene, determined its expression pattern, and tested its role in cold resistance. We obtained the full-length P. lactiflora GPAT gene using tissue-cultured seedlings and real-time polymerase chain reaction and rapid amplification of cDNA ends analyses. We named this gene PlGPAT in P. lactiflora. Phylogenetic analysis indicates that the PlGPAT gene is closely related with the GPAT genes in core eudicots. The phylogenetic tree containing 31 angiosperm species based on GPAT protein sequences is largely consistent with the known phylogeny in flowering plants. We conducted a time-course PlGPAT expression analysis and demonstrated that PlGPAT expression is correlated with low-temperature stress. Our results suggest that the PlGPAT gene plays an important role in regulating cold resistance in P. lactiflora. |
format | Online Article Text |
id | pubmed-6086452 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-60864522018-08-28 Characterization of the glycerol-3-phosphate acyltransferase gene and its real-time expression under cold stress in Paeonia lactiflora Pall Li, Xueting Liu, Ping Yang, Panpan Fan, Chuanzhu Sun, Xiaomei PLoS One Research Article Elucidating the cold tolerance mechanism of Paeonia lactiflora, which is one of the most valuable ornamental and medicinal plants in Asia, fundamentally impacts its breeding and production. The glycerol-3-phosphate acyltransferase (GPAT) gene plays a pivotal role in cold resistance in a variety of plant species. Here, we cloned the P. lactiflora GPAT gene, determined its expression pattern, and tested its role in cold resistance. We obtained the full-length P. lactiflora GPAT gene using tissue-cultured seedlings and real-time polymerase chain reaction and rapid amplification of cDNA ends analyses. We named this gene PlGPAT in P. lactiflora. Phylogenetic analysis indicates that the PlGPAT gene is closely related with the GPAT genes in core eudicots. The phylogenetic tree containing 31 angiosperm species based on GPAT protein sequences is largely consistent with the known phylogeny in flowering plants. We conducted a time-course PlGPAT expression analysis and demonstrated that PlGPAT expression is correlated with low-temperature stress. Our results suggest that the PlGPAT gene plays an important role in regulating cold resistance in P. lactiflora. Public Library of Science 2018-08-10 /pmc/articles/PMC6086452/ /pubmed/30096187 http://dx.doi.org/10.1371/journal.pone.0202168 Text en © 2018 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Li, Xueting Liu, Ping Yang, Panpan Fan, Chuanzhu Sun, Xiaomei Characterization of the glycerol-3-phosphate acyltransferase gene and its real-time expression under cold stress in Paeonia lactiflora Pall |
title | Characterization of the glycerol-3-phosphate acyltransferase gene and its real-time expression under cold stress in Paeonia lactiflora Pall |
title_full | Characterization of the glycerol-3-phosphate acyltransferase gene and its real-time expression under cold stress in Paeonia lactiflora Pall |
title_fullStr | Characterization of the glycerol-3-phosphate acyltransferase gene and its real-time expression under cold stress in Paeonia lactiflora Pall |
title_full_unstemmed | Characterization of the glycerol-3-phosphate acyltransferase gene and its real-time expression under cold stress in Paeonia lactiflora Pall |
title_short | Characterization of the glycerol-3-phosphate acyltransferase gene and its real-time expression under cold stress in Paeonia lactiflora Pall |
title_sort | characterization of the glycerol-3-phosphate acyltransferase gene and its real-time expression under cold stress in paeonia lactiflora pall |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6086452/ https://www.ncbi.nlm.nih.gov/pubmed/30096187 http://dx.doi.org/10.1371/journal.pone.0202168 |
work_keys_str_mv | AT lixueting characterizationoftheglycerol3phosphateacyltransferasegeneanditsrealtimeexpressionundercoldstressinpaeonialactiflorapall AT liuping characterizationoftheglycerol3phosphateacyltransferasegeneanditsrealtimeexpressionundercoldstressinpaeonialactiflorapall AT yangpanpan characterizationoftheglycerol3phosphateacyltransferasegeneanditsrealtimeexpressionundercoldstressinpaeonialactiflorapall AT fanchuanzhu characterizationoftheglycerol3phosphateacyltransferasegeneanditsrealtimeexpressionundercoldstressinpaeonialactiflorapall AT sunxiaomei characterizationoftheglycerol3phosphateacyltransferasegeneanditsrealtimeexpressionundercoldstressinpaeonialactiflorapall |