An attenuated replication-competent chikungunya virus with a fluorescently tagged envelope

BACKGROUND: Chikungunya virus (CHIKV) is the most common alphavirus infecting humans worldwide, causing acute and chronically debilitating arthralgia at a great economic expense. METHODOLOGY/PRINCIPAL FINDINGS: To facilitate our study of CHIKV, we generated a mCherry tagged replication-competent chi...

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Detalles Bibliográficos
Autores principales: Jin, Jing, Sherman, Michael B., Chafets, Daniel, Dinglasan, Nuntana, Lu, Kai, Lee, Tzong-Hae, Carlson, Lars-Anders, Muench, Marcus O., Simmons, Graham
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6086482/
https://www.ncbi.nlm.nih.gov/pubmed/30063703
http://dx.doi.org/10.1371/journal.pntd.0006693
Descripción
Sumario:BACKGROUND: Chikungunya virus (CHIKV) is the most common alphavirus infecting humans worldwide, causing acute and chronically debilitating arthralgia at a great economic expense. METHODOLOGY/PRINCIPAL FINDINGS: To facilitate our study of CHIKV, we generated a mCherry tagged replication-competent chimeric virus, CHIKV 37997-mCherry. Single particle cryoEM demonstrated icosahedral organization of the chimeric virus and the display of mCherry proteins on virus surface. CHIKV 37997-mCherry is attenuated in both IFNαR knockout and wild-type mice. Strong anti-CHIKV and anti-mCherry antibody responses were induced in CHIKV 37997-mCherry infected mice. CONCLUSIONS/SIGNIFICANCE: Our work suggests that chimeric alphaviruses displaying foreign antigen can serve as vaccines against both aphaviruses and other pathogens and diseases.

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