MicroRNA-488 inhibits proliferation, invasion and EMT in osteosarcoma cell lines by targeting aquaporin 3

It has been reported that aquaporin 3 (AQP3) expression is associated with the progression of numerous types of cancer and microRNA (miRNA/miR) processing. However, the effects and precise mechanisms of AQP3 in osteosarcoma (OS) have not been fully elucidated. The present study aimed to investigate the interaction between AQP3 and miR-488 in OS. The reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay was performed to detect the levels of AQP3 and miR-488 in OS tissues and cell lines, respectively. Cell proliferation, invasion and epithelial-mesenchymal transition (EMT) were detected to analyze the biological functions of miR-488 and AQP3 in OS cells. Furthermore, mRNA and protein levels of AQP3 was measured by RT-qPCR and western blot analysis. Furthermore, AQP3 was validated as an miR-488 target using luciferase assays in OS cells. The present study revealed that the miR-488 level was significantly downregulated in OS tissues and cell lines, and that the expression of AQP3 was markedly increased. Notable, the low miR-488 expression level was associated with upregulated AQP3 expression in OS tissues. Furthermore, introduction of miR-488 markedly suppressed the proliferation, invasion and EMT of OS cells. However, miR-488-knockdown increased the proliferation, invasion and EMT of OS cells. The present study demonstrated that miR-488 could directly target AQP3 using bioinformatics analysis and luciferase reporter assays. In addition, AQP3-silencing had similar effects to miR-488 overexpression on OS cells. Overexpression of AQP3 in OS cells partially reversed the inhibitory effects of miR-488 mimic. miR-488 inhibited the proliferation, invasion and EMT of OS cells by directly downregulating AQP3 expression, and miR-488 targeting AQP3 was responsible for inhibition of the proliferation, invasion and EMT of OS cells.