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Efficient analysis of mammalian polysomes in cells and tissues using Ribo Mega-SEC

We describe Ribo Mega-SEC, a powerful approach for the separation and biochemical analysis of mammalian polysomes and ribosomal subunits using Size Exclusion Chromatography and uHPLC. Using extracts from either cells, or tissues, polysomes can be separated within 15 min from sample injection to frac...

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Detalles Bibliográficos
Autores principales:	Yoshikawa, Harunori, Larance, Mark, Harney, Dylan J, Sundaramoorthy, Ramasubramanian, Ly, Tony, Owen-Hughes, Tom, Lamond, Angus I
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	eLife Sciences Publications, Ltd 2018
Materias:	Biochemistry and Chemical Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6086667/ https://www.ncbi.nlm.nih.gov/pubmed/30095066 http://dx.doi.org/10.7554/eLife.36530

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