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Nuclear-resident RIG-I senses viral replication inducing antiviral immunity

The nucleus represents a cellular compartment where the discrimination of self from non-self nucleic acids is vital. While emerging evidence establishes a nuclear non-self DNA sensing paradigm, the nuclear sensing of non-self RNA, such as that from nuclear-replicating RNA viruses, remains unexplored...

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Autores principales: Liu, GuanQun, Lu, Yao, Thulasi Raman, Sathya N., Xu, Fang, Wu, Qi, Li, Zhubing, Brownlie, Robert, Liu, Qiang, Zhou, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6086882/
https://www.ncbi.nlm.nih.gov/pubmed/30097581
http://dx.doi.org/10.1038/s41467-018-05745-w
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author Liu, GuanQun
Lu, Yao
Thulasi Raman, Sathya N.
Xu, Fang
Wu, Qi
Li, Zhubing
Brownlie, Robert
Liu, Qiang
Zhou, Yan
author_facet Liu, GuanQun
Lu, Yao
Thulasi Raman, Sathya N.
Xu, Fang
Wu, Qi
Li, Zhubing
Brownlie, Robert
Liu, Qiang
Zhou, Yan
author_sort Liu, GuanQun
collection PubMed
description The nucleus represents a cellular compartment where the discrimination of self from non-self nucleic acids is vital. While emerging evidence establishes a nuclear non-self DNA sensing paradigm, the nuclear sensing of non-self RNA, such as that from nuclear-replicating RNA viruses, remains unexplored. Here, we report the identification of nuclear-resident RIG-I actively involved in nuclear viral RNA sensing. The nuclear RIG-I, along with its cytoplasmic counterpart, senses influenza A virus (IAV) nuclear replication leading to a cooperative induction of type I interferon response. Its activation signals through the canonical signaling axis and establishes an effective antiviral state restricting IAV replication. The exclusive signaling specificity conferred by nuclear RIG-I is reinforced by its inability to sense cytoplasmic-replicating Sendai virus and appreciable sensing of hepatitis B virus pregenomic RNA in the nucleus. These results refine the RNA sensing paradigm for nuclear-replicating viruses and reveal a previously unrecognized subcellular milieu for RIG-I-like receptor sensing.
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spelling pubmed-60868822018-08-13 Nuclear-resident RIG-I senses viral replication inducing antiviral immunity Liu, GuanQun Lu, Yao Thulasi Raman, Sathya N. Xu, Fang Wu, Qi Li, Zhubing Brownlie, Robert Liu, Qiang Zhou, Yan Nat Commun Article The nucleus represents a cellular compartment where the discrimination of self from non-self nucleic acids is vital. While emerging evidence establishes a nuclear non-self DNA sensing paradigm, the nuclear sensing of non-self RNA, such as that from nuclear-replicating RNA viruses, remains unexplored. Here, we report the identification of nuclear-resident RIG-I actively involved in nuclear viral RNA sensing. The nuclear RIG-I, along with its cytoplasmic counterpart, senses influenza A virus (IAV) nuclear replication leading to a cooperative induction of type I interferon response. Its activation signals through the canonical signaling axis and establishes an effective antiviral state restricting IAV replication. The exclusive signaling specificity conferred by nuclear RIG-I is reinforced by its inability to sense cytoplasmic-replicating Sendai virus and appreciable sensing of hepatitis B virus pregenomic RNA in the nucleus. These results refine the RNA sensing paradigm for nuclear-replicating viruses and reveal a previously unrecognized subcellular milieu for RIG-I-like receptor sensing. Nature Publishing Group UK 2018-08-10 /pmc/articles/PMC6086882/ /pubmed/30097581 http://dx.doi.org/10.1038/s41467-018-05745-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Liu, GuanQun
Lu, Yao
Thulasi Raman, Sathya N.
Xu, Fang
Wu, Qi
Li, Zhubing
Brownlie, Robert
Liu, Qiang
Zhou, Yan
Nuclear-resident RIG-I senses viral replication inducing antiviral immunity
title Nuclear-resident RIG-I senses viral replication inducing antiviral immunity
title_full Nuclear-resident RIG-I senses viral replication inducing antiviral immunity
title_fullStr Nuclear-resident RIG-I senses viral replication inducing antiviral immunity
title_full_unstemmed Nuclear-resident RIG-I senses viral replication inducing antiviral immunity
title_short Nuclear-resident RIG-I senses viral replication inducing antiviral immunity
title_sort nuclear-resident rig-i senses viral replication inducing antiviral immunity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6086882/
https://www.ncbi.nlm.nih.gov/pubmed/30097581
http://dx.doi.org/10.1038/s41467-018-05745-w
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