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Let-7b contributes to hepatocellular cancer progression through Wnt/β-catenin signaling
Elevated evidences show that microRNAs (miRNAs) play vital roles in tumor progression regulation. However, the functional role of let-7b in hepatocellular carcinoma (HCC) is still largely unknown. In this study, we try to investigate the biological activity of let-7b in human HCC cells and try to fi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6087813/ https://www.ncbi.nlm.nih.gov/pubmed/30108446 http://dx.doi.org/10.1016/j.sjbs.2018.03.004 |
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author | Wang, Yiwei Mo, Yanbo Wang, Lin Su, Peng Xie, Yuxi |
author_facet | Wang, Yiwei Mo, Yanbo Wang, Lin Su, Peng Xie, Yuxi |
author_sort | Wang, Yiwei |
collection | PubMed |
description | Elevated evidences show that microRNAs (miRNAs) play vital roles in tumor progression regulation. However, the functional role of let-7b in hepatocellular carcinoma (HCC) is still largely unknown. In this study, we try to investigate the biological activity of let-7b in human HCC cells and try to find the potential regulatory signaling pathway. Our results indicate that let- 7b was remarkably down-regulated in human HCC tissues by qRT-PCR. In addition, let-7b overexpression decreased the expression of β-catenin and c-Myc, while upregulated E-cadherin expression in HCC cells which was verified by quantitative real-time PCR (qRT-PCR) and western blotting. Furthermore, Wnt/β-catenin was involved in let-7b biological activity which was revealed by luciferase assay. Moreover, Wnt/β-catenin signaling inhibitor blocks HCC cell proliferation which is as the same pattern as let-7b overexpression inhibits in HCC cells proliferation. In conclusion, down-regulated let-7b promotes HCC cell proliferation through Wnt/β-catenin signaling in HCC cells. These results suggested that appropriate manipulation of let-7b might be a new treatment of human HCC in the future. |
format | Online Article Text |
id | pubmed-6087813 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-60878132018-08-14 Let-7b contributes to hepatocellular cancer progression through Wnt/β-catenin signaling Wang, Yiwei Mo, Yanbo Wang, Lin Su, Peng Xie, Yuxi Saudi J Biol Sci Article Elevated evidences show that microRNAs (miRNAs) play vital roles in tumor progression regulation. However, the functional role of let-7b in hepatocellular carcinoma (HCC) is still largely unknown. In this study, we try to investigate the biological activity of let-7b in human HCC cells and try to find the potential regulatory signaling pathway. Our results indicate that let- 7b was remarkably down-regulated in human HCC tissues by qRT-PCR. In addition, let-7b overexpression decreased the expression of β-catenin and c-Myc, while upregulated E-cadherin expression in HCC cells which was verified by quantitative real-time PCR (qRT-PCR) and western blotting. Furthermore, Wnt/β-catenin was involved in let-7b biological activity which was revealed by luciferase assay. Moreover, Wnt/β-catenin signaling inhibitor blocks HCC cell proliferation which is as the same pattern as let-7b overexpression inhibits in HCC cells proliferation. In conclusion, down-regulated let-7b promotes HCC cell proliferation through Wnt/β-catenin signaling in HCC cells. These results suggested that appropriate manipulation of let-7b might be a new treatment of human HCC in the future. Elsevier 2018-07 2018-03-06 /pmc/articles/PMC6087813/ /pubmed/30108446 http://dx.doi.org/10.1016/j.sjbs.2018.03.004 Text en © 2018 Production and hosting by Elsevier B.V. on behalf of King Saud University. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Wang, Yiwei Mo, Yanbo Wang, Lin Su, Peng Xie, Yuxi Let-7b contributes to hepatocellular cancer progression through Wnt/β-catenin signaling |
title | Let-7b contributes to hepatocellular cancer progression through Wnt/β-catenin signaling |
title_full | Let-7b contributes to hepatocellular cancer progression through Wnt/β-catenin signaling |
title_fullStr | Let-7b contributes to hepatocellular cancer progression through Wnt/β-catenin signaling |
title_full_unstemmed | Let-7b contributes to hepatocellular cancer progression through Wnt/β-catenin signaling |
title_short | Let-7b contributes to hepatocellular cancer progression through Wnt/β-catenin signaling |
title_sort | let-7b contributes to hepatocellular cancer progression through wnt/β-catenin signaling |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6087813/ https://www.ncbi.nlm.nih.gov/pubmed/30108446 http://dx.doi.org/10.1016/j.sjbs.2018.03.004 |
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