MicroRNA-4728 serves as a suppressor and antagonist of oncogenic MAPK in Burkitt lymphoma

OBJECTIVE: This study identified the biological role of miR-4728 in Burkitt lymphoma (BL) process. METHODS: Ramos cells were used to analyze MicroRNA-4728 (miR-4728) biological functions. MiR-4728 expression was investigated in 14 randomly chosen tumor tissues and 12 noncancerous tissues by qRT-PCR....

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Detalles Bibliográficos
Autores principales: Wang, Wei, Lin, Peng, Yao, Haiying, Jia, Xi, Sun, Jirui, Feng, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6088107/
https://www.ncbi.nlm.nih.gov/pubmed/30108451
http://dx.doi.org/10.1016/j.sjbs.2018.05.010
Descripción
Sumario:OBJECTIVE: This study identified the biological role of miR-4728 in Burkitt lymphoma (BL) process. METHODS: Ramos cells were used to analyze MicroRNA-4728 (miR-4728) biological functions. MiR-4728 expression was investigated in 14 randomly chosen tumor tissues and 12 noncancerous tissues by qRT-PCR. Cyquant assay was used to monitor cell proliferation. Colony formation assay was performed to study the effectiveness of miR-4728 on the proliferation of cells. The effects of miR-4728 on MAPK signaling pathway were detected by luciferase reporter assay. The significance of differences between groups were evaluated by SPSS. RESULTS: In this study, MiRNA-4728 was observed to down-regulated in BL tissues compared to the noncancerous tissues. Additionally, miR-4728 inhibited Ramos cell proliferation. Moreover, miR-4728 overexpression also decreased the MAPK signaling activity. CONCLUSION: Our results suggested that miR-4728 serves as a suppressor and antagonist of oncogenic MAPK in Burkitt lymphoma. The appropriate regulation of miR-4728 might be vital to improve BL treatment.

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