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The Microtubule Regulator NEK7 Coordinates the Wiring of Cortical Parvalbumin Interneurons
Functional networks in the mammalian cerebral cortex rely on the interaction between glutamatergic pyramidal cells and GABAergic interneurons. Both neuronal populations exhibit an extraordinary divergence in morphology and targeting areas, which ultimately dictate their precise function in cortical...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6088228/ https://www.ncbi.nlm.nih.gov/pubmed/30067978 http://dx.doi.org/10.1016/j.celrep.2018.06.115 |
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author | Hinojosa, Antonio Jesús Deogracias, Rubén Rico, Beatriz |
author_facet | Hinojosa, Antonio Jesús Deogracias, Rubén Rico, Beatriz |
author_sort | Hinojosa, Antonio Jesús |
collection | PubMed |
description | Functional networks in the mammalian cerebral cortex rely on the interaction between glutamatergic pyramidal cells and GABAergic interneurons. Both neuronal populations exhibit an extraordinary divergence in morphology and targeting areas, which ultimately dictate their precise function in cortical circuits. How these prominent morphological differences arise during development is not well understood. Here, we conducted a high-throughput screen for genes differentially expressed by pyramidal cells and interneurons during cortical wiring. We found that NEK7, a kinase involved in microtubule polymerization, is mostly expressed in parvalbumin (PV+) interneurons at the time when they establish their connectivity. Functional experiments revealed that NEK7-deficient PV+ interneurons show altered microtubule dynamics, axon growth cone steering and reduced axon length, arbor complexity, and total number of synaptic contacts formed with pyramidal cells. Altogether, our results reveal a molecular mechanism by which the microtubule-associated kinase NEK7 regulates the wiring of PV+ interneurons. |
format | Online Article Text |
id | pubmed-6088228 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-60882282018-08-14 The Microtubule Regulator NEK7 Coordinates the Wiring of Cortical Parvalbumin Interneurons Hinojosa, Antonio Jesús Deogracias, Rubén Rico, Beatriz Cell Rep Article Functional networks in the mammalian cerebral cortex rely on the interaction between glutamatergic pyramidal cells and GABAergic interneurons. Both neuronal populations exhibit an extraordinary divergence in morphology and targeting areas, which ultimately dictate their precise function in cortical circuits. How these prominent morphological differences arise during development is not well understood. Here, we conducted a high-throughput screen for genes differentially expressed by pyramidal cells and interneurons during cortical wiring. We found that NEK7, a kinase involved in microtubule polymerization, is mostly expressed in parvalbumin (PV+) interneurons at the time when they establish their connectivity. Functional experiments revealed that NEK7-deficient PV+ interneurons show altered microtubule dynamics, axon growth cone steering and reduced axon length, arbor complexity, and total number of synaptic contacts formed with pyramidal cells. Altogether, our results reveal a molecular mechanism by which the microtubule-associated kinase NEK7 regulates the wiring of PV+ interneurons. Cell Press 2018-07-31 /pmc/articles/PMC6088228/ /pubmed/30067978 http://dx.doi.org/10.1016/j.celrep.2018.06.115 Text en Crown Copyright © 2018 Published by Elsevier Inc. All rights reserved. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hinojosa, Antonio Jesús Deogracias, Rubén Rico, Beatriz The Microtubule Regulator NEK7 Coordinates the Wiring of Cortical Parvalbumin Interneurons |
title | The Microtubule Regulator NEK7 Coordinates the Wiring of Cortical Parvalbumin Interneurons |
title_full | The Microtubule Regulator NEK7 Coordinates the Wiring of Cortical Parvalbumin Interneurons |
title_fullStr | The Microtubule Regulator NEK7 Coordinates the Wiring of Cortical Parvalbumin Interneurons |
title_full_unstemmed | The Microtubule Regulator NEK7 Coordinates the Wiring of Cortical Parvalbumin Interneurons |
title_short | The Microtubule Regulator NEK7 Coordinates the Wiring of Cortical Parvalbumin Interneurons |
title_sort | microtubule regulator nek7 coordinates the wiring of cortical parvalbumin interneurons |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6088228/ https://www.ncbi.nlm.nih.gov/pubmed/30067978 http://dx.doi.org/10.1016/j.celrep.2018.06.115 |
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