Cargando…
Luminal MCF-12A & myoepithelial-like Hs 578Bst cells form bilayered acini similar to human breast
The epithelium's functional unit is the bilayered acinus, made of a layer of luminal cells, surrounded by a layer of basal cells mainly composed of myoepithelial cells. AIM: The aim of this study was to develop a reproducible and manipulable 3D co-culture model of the bilayered acinus in vitro...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Future Science Ltd
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6088263/ https://www.ncbi.nlm.nih.gov/pubmed/30112185 http://dx.doi.org/10.4155/fsoa-2018-0010 |
_version_ | 1783346813595549696 |
---|---|
author | Weber-Ouellette, Anne Busby, Mélanie Plante, Isabelle |
author_facet | Weber-Ouellette, Anne Busby, Mélanie Plante, Isabelle |
author_sort | Weber-Ouellette, Anne |
collection | PubMed |
description | The epithelium's functional unit is the bilayered acinus, made of a layer of luminal cells, surrounded by a layer of basal cells mainly composed of myoepithelial cells. AIM: The aim of this study was to develop a reproducible and manipulable 3D co-culture model of the bilayered acinus in vitro to study the interactions between the two layers. MATERIALS & METHODS: Two different combinations of cell lines were co-cultured in Matrigel: SCp2 and SCg6 mice cells, or MCF-12A and Hs 578Bst human cell lines. RESULTS: Confocal microscopy analysis showed that only MCF-12A and Hs 578Bst cells could form some bilayered acini. This in vitro bilayered acini model will allow us to understand the role of interactions between luminal and myoepithelial cells in the normal breast development. |
format | Online Article Text |
id | pubmed-6088263 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Future Science Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-60882632018-08-15 Luminal MCF-12A & myoepithelial-like Hs 578Bst cells form bilayered acini similar to human breast Weber-Ouellette, Anne Busby, Mélanie Plante, Isabelle Future Sci OA Research Article The epithelium's functional unit is the bilayered acinus, made of a layer of luminal cells, surrounded by a layer of basal cells mainly composed of myoepithelial cells. AIM: The aim of this study was to develop a reproducible and manipulable 3D co-culture model of the bilayered acinus in vitro to study the interactions between the two layers. MATERIALS & METHODS: Two different combinations of cell lines were co-cultured in Matrigel: SCp2 and SCg6 mice cells, or MCF-12A and Hs 578Bst human cell lines. RESULTS: Confocal microscopy analysis showed that only MCF-12A and Hs 578Bst cells could form some bilayered acini. This in vitro bilayered acini model will allow us to understand the role of interactions between luminal and myoepithelial cells in the normal breast development. Future Science Ltd 2018-06-28 /pmc/articles/PMC6088263/ /pubmed/30112185 http://dx.doi.org/10.4155/fsoa-2018-0010 Text en © 2018 Isabelle Plante This work is licensed under a Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/) |
spellingShingle | Research Article Weber-Ouellette, Anne Busby, Mélanie Plante, Isabelle Luminal MCF-12A & myoepithelial-like Hs 578Bst cells form bilayered acini similar to human breast |
title | Luminal MCF-12A & myoepithelial-like Hs 578Bst cells form bilayered acini similar to human breast |
title_full | Luminal MCF-12A & myoepithelial-like Hs 578Bst cells form bilayered acini similar to human breast |
title_fullStr | Luminal MCF-12A & myoepithelial-like Hs 578Bst cells form bilayered acini similar to human breast |
title_full_unstemmed | Luminal MCF-12A & myoepithelial-like Hs 578Bst cells form bilayered acini similar to human breast |
title_short | Luminal MCF-12A & myoepithelial-like Hs 578Bst cells form bilayered acini similar to human breast |
title_sort | luminal mcf-12a & myoepithelial-like hs 578bst cells form bilayered acini similar to human breast |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6088263/ https://www.ncbi.nlm.nih.gov/pubmed/30112185 http://dx.doi.org/10.4155/fsoa-2018-0010 |
work_keys_str_mv | AT weberouelletteanne luminalmcf12amyoepitheliallikehs578bstcellsformbilayeredacinisimilartohumanbreast AT busbymelanie luminalmcf12amyoepitheliallikehs578bstcellsformbilayeredacinisimilartohumanbreast AT planteisabelle luminalmcf12amyoepitheliallikehs578bstcellsformbilayeredacinisimilartohumanbreast |