Cargando…
De novo transcriptomic assembly and mRNA expression patterns of Botryosphaeria dothidea infection with mycoviruses chrysovirus 1 (BdCV1) and partitivirus 1 (BdPV1)
BACKGROUND: Pear ring rot, caused by Botryosphaeria species, is responsible for substantial economic losses by causing severe recession of pear tree growth in China. Mycovirus-mediated hypovirulence in plant pathogenic fungi is a crucial biological method to control fungal diseases. METHODS: We cond...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6088430/ https://www.ncbi.nlm.nih.gov/pubmed/30103770 http://dx.doi.org/10.1186/s12985-018-1033-4 |
_version_ | 1783346832192045056 |
---|---|
author | Wang, Lihua Luo, Hui Hu, Wangcheng Yang, Yuekun Hong, Ni Wang, Guoping Wang, Aiming Wang, Liping |
author_facet | Wang, Lihua Luo, Hui Hu, Wangcheng Yang, Yuekun Hong, Ni Wang, Guoping Wang, Aiming Wang, Liping |
author_sort | Wang, Lihua |
collection | PubMed |
description | BACKGROUND: Pear ring rot, caused by Botryosphaeria species, is responsible for substantial economic losses by causing severe recession of pear tree growth in China. Mycovirus-mediated hypovirulence in plant pathogenic fungi is a crucial biological method to control fungal diseases. METHODS: We conducted a large-scale and comprehensive transcriptome analysis to identify mRNA in B. dothidea in response to mycovirus. De novo sequencing technology from four constructed libraries of LW-C (Botryosphaeria dothidea chrysovirus 1, BdCV1), LW-P (Botryosphaeria dothidea partitivirus 1, BdPV1), LW-CP (LW-1 strain infection with BdCV1 and BdPV1), and Mock (free virus) was used to investigate and compare gene expression changes in B.dothidea strains infected with mycovirus. RESULTS: In total, 30,058 Unigenes with an average length of 2128 bp were obtained from 4 libraries of B. dothidea strains. These were annotated to specify their classified function. We demonstrate that mRNAs of B. dothidea strains in response to mycovirus are differentially expressed. In total, 5598 genes were up-regulated and 3298 were down-regulated in the LW-CP group, 4468 were up-regulated and 4291 down-regulated in the LW-C group, and 2590 were up-regulated and 2325 down-regulated in the LW-P group. RT-qPCR was used to validate the expression of 9 selected genes. The B. dothidea transcriptome was more affected by BdCV1 infection than BdPV1. We conducted GO enrichment analysis to characterize gene functions regulated by B. dothidea with mycovirus infection. These involved metabolic process, cellular process, catalytic activity, transporter activity, signaling, and other biological pathways. KEGG function analysis demonstrated that the enriched differentially expressed genes are involved in metabolism, transcription, signal transduction, and ABC transport. mRNA is therefore involved in the interaction between fungi and mycovirus. In addition, changes in differential accumulation levels of cp and RdRp of BdCV1 and BdPV1 in B. dothidea strains were evaluated, revealing that the accumulation of BdCV1 and BdPV1 is related to the phenotype and virulence of B. dothidea strain LW-1. CONCLUSIONS: The identification and analysis of mRNAs from B. dothidea was first reported at the transcriptome level. Our analysis provides further insight into the interaction of B. dothidea strains infection with chrysovirus 1 (BdCV1) and partitivirus 1 (BdPV1) at the transcriptome level. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12985-018-1033-4) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6088430 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-60884302018-08-17 De novo transcriptomic assembly and mRNA expression patterns of Botryosphaeria dothidea infection with mycoviruses chrysovirus 1 (BdCV1) and partitivirus 1 (BdPV1) Wang, Lihua Luo, Hui Hu, Wangcheng Yang, Yuekun Hong, Ni Wang, Guoping Wang, Aiming Wang, Liping Virol J Research BACKGROUND: Pear ring rot, caused by Botryosphaeria species, is responsible for substantial economic losses by causing severe recession of pear tree growth in China. Mycovirus-mediated hypovirulence in plant pathogenic fungi is a crucial biological method to control fungal diseases. METHODS: We conducted a large-scale and comprehensive transcriptome analysis to identify mRNA in B. dothidea in response to mycovirus. De novo sequencing technology from four constructed libraries of LW-C (Botryosphaeria dothidea chrysovirus 1, BdCV1), LW-P (Botryosphaeria dothidea partitivirus 1, BdPV1), LW-CP (LW-1 strain infection with BdCV1 and BdPV1), and Mock (free virus) was used to investigate and compare gene expression changes in B.dothidea strains infected with mycovirus. RESULTS: In total, 30,058 Unigenes with an average length of 2128 bp were obtained from 4 libraries of B. dothidea strains. These were annotated to specify their classified function. We demonstrate that mRNAs of B. dothidea strains in response to mycovirus are differentially expressed. In total, 5598 genes were up-regulated and 3298 were down-regulated in the LW-CP group, 4468 were up-regulated and 4291 down-regulated in the LW-C group, and 2590 were up-regulated and 2325 down-regulated in the LW-P group. RT-qPCR was used to validate the expression of 9 selected genes. The B. dothidea transcriptome was more affected by BdCV1 infection than BdPV1. We conducted GO enrichment analysis to characterize gene functions regulated by B. dothidea with mycovirus infection. These involved metabolic process, cellular process, catalytic activity, transporter activity, signaling, and other biological pathways. KEGG function analysis demonstrated that the enriched differentially expressed genes are involved in metabolism, transcription, signal transduction, and ABC transport. mRNA is therefore involved in the interaction between fungi and mycovirus. In addition, changes in differential accumulation levels of cp and RdRp of BdCV1 and BdPV1 in B. dothidea strains were evaluated, revealing that the accumulation of BdCV1 and BdPV1 is related to the phenotype and virulence of B. dothidea strain LW-1. CONCLUSIONS: The identification and analysis of mRNAs from B. dothidea was first reported at the transcriptome level. Our analysis provides further insight into the interaction of B. dothidea strains infection with chrysovirus 1 (BdCV1) and partitivirus 1 (BdPV1) at the transcriptome level. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12985-018-1033-4) contains supplementary material, which is available to authorized users. BioMed Central 2018-08-13 /pmc/articles/PMC6088430/ /pubmed/30103770 http://dx.doi.org/10.1186/s12985-018-1033-4 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Wang, Lihua Luo, Hui Hu, Wangcheng Yang, Yuekun Hong, Ni Wang, Guoping Wang, Aiming Wang, Liping De novo transcriptomic assembly and mRNA expression patterns of Botryosphaeria dothidea infection with mycoviruses chrysovirus 1 (BdCV1) and partitivirus 1 (BdPV1) |
title | De novo transcriptomic assembly and mRNA expression patterns of Botryosphaeria dothidea infection with mycoviruses chrysovirus 1 (BdCV1) and partitivirus 1 (BdPV1) |
title_full | De novo transcriptomic assembly and mRNA expression patterns of Botryosphaeria dothidea infection with mycoviruses chrysovirus 1 (BdCV1) and partitivirus 1 (BdPV1) |
title_fullStr | De novo transcriptomic assembly and mRNA expression patterns of Botryosphaeria dothidea infection with mycoviruses chrysovirus 1 (BdCV1) and partitivirus 1 (BdPV1) |
title_full_unstemmed | De novo transcriptomic assembly and mRNA expression patterns of Botryosphaeria dothidea infection with mycoviruses chrysovirus 1 (BdCV1) and partitivirus 1 (BdPV1) |
title_short | De novo transcriptomic assembly and mRNA expression patterns of Botryosphaeria dothidea infection with mycoviruses chrysovirus 1 (BdCV1) and partitivirus 1 (BdPV1) |
title_sort | de novo transcriptomic assembly and mrna expression patterns of botryosphaeria dothidea infection with mycoviruses chrysovirus 1 (bdcv1) and partitivirus 1 (bdpv1) |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6088430/ https://www.ncbi.nlm.nih.gov/pubmed/30103770 http://dx.doi.org/10.1186/s12985-018-1033-4 |
work_keys_str_mv | AT wanglihua denovotranscriptomicassemblyandmrnaexpressionpatternsofbotryosphaeriadothideainfectionwithmycoviruseschrysovirus1bdcv1andpartitivirus1bdpv1 AT luohui denovotranscriptomicassemblyandmrnaexpressionpatternsofbotryosphaeriadothideainfectionwithmycoviruseschrysovirus1bdcv1andpartitivirus1bdpv1 AT huwangcheng denovotranscriptomicassemblyandmrnaexpressionpatternsofbotryosphaeriadothideainfectionwithmycoviruseschrysovirus1bdcv1andpartitivirus1bdpv1 AT yangyuekun denovotranscriptomicassemblyandmrnaexpressionpatternsofbotryosphaeriadothideainfectionwithmycoviruseschrysovirus1bdcv1andpartitivirus1bdpv1 AT hongni denovotranscriptomicassemblyandmrnaexpressionpatternsofbotryosphaeriadothideainfectionwithmycoviruseschrysovirus1bdcv1andpartitivirus1bdpv1 AT wangguoping denovotranscriptomicassemblyandmrnaexpressionpatternsofbotryosphaeriadothideainfectionwithmycoviruseschrysovirus1bdcv1andpartitivirus1bdpv1 AT wangaiming denovotranscriptomicassemblyandmrnaexpressionpatternsofbotryosphaeriadothideainfectionwithmycoviruseschrysovirus1bdcv1andpartitivirus1bdpv1 AT wangliping denovotranscriptomicassemblyandmrnaexpressionpatternsofbotryosphaeriadothideainfectionwithmycoviruseschrysovirus1bdcv1andpartitivirus1bdpv1 |