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Variability of core microbiota in newly diagnosed treatment-naïve paediatric inflammatory bowel disease patients

BACKGROUND & AIMS: Intestinal microbiota is considered to play a crucial role in the aetiology of inflammatory bowel disease (IBD). We aimed to describe faecal microbiota composition and dynamics in a large cohort of children with de novo (naïve) IBD, in comparison to healthy paediatric controls...

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Autores principales: de Meij, T. G. J., de Groot, E. F. J., Peeters, C. F. W., de Boer, N. K. H., Kneepkens, C. M. F., Eck, A., Benninga, M. A., Savelkoul, P. H. M., van Bodegraven, A. A., Budding, A. E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6089417/
https://www.ncbi.nlm.nih.gov/pubmed/30102706
http://dx.doi.org/10.1371/journal.pone.0197649
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author de Meij, T. G. J.
de Groot, E. F. J.
Peeters, C. F. W.
de Boer, N. K. H.
Kneepkens, C. M. F.
Eck, A.
Benninga, M. A.
Savelkoul, P. H. M.
van Bodegraven, A. A.
Budding, A. E.
author_facet de Meij, T. G. J.
de Groot, E. F. J.
Peeters, C. F. W.
de Boer, N. K. H.
Kneepkens, C. M. F.
Eck, A.
Benninga, M. A.
Savelkoul, P. H. M.
van Bodegraven, A. A.
Budding, A. E.
author_sort de Meij, T. G. J.
collection PubMed
description BACKGROUND & AIMS: Intestinal microbiota is considered to play a crucial role in the aetiology of inflammatory bowel disease (IBD). We aimed to describe faecal microbiota composition and dynamics in a large cohort of children with de novo (naïve) IBD, in comparison to healthy paediatric controls (HC). METHODS: In this prospective study, performed at two tertiary centres, faecal samples from newly diagnosed, treatment-naïve paediatric IBD patients were collected prior to bowel cleansing for colonoscopy (t0) and 1, 3 and 6 weeks and 3 months after initiation of therapy. The microbial profiles of Crohn’s disease (CD) and Ulcerative colitis (UC) patients were compared with HC and linked to therapeutic response. Microbiota composition was analysed by IS-pro technology. RESULTS: Microbial profiles of 104 new IBD-patients (63 CD, 41 UC, median age 14.0 years) were compared to 61 HC (median 7.8 years). IBD was mainly characterised by decreased abundance of Alistipes finegoldii and Alistipes putredinis, which characterize a healthy state microbial core. The classifier including these core species as predictors achieved an AUC of the ROC curve of .87. Core bacteria tended to regain abundance during treatment, but did not reach healthy levels. CONCLUSION: Faecal microbiota profiles of children with de novo CD and UC can be discriminated from HC with high accuracy, mainly driven by a decreased abundance of species shaping the microbial core in the healthy state. Paediatric IBD can therefore be characterized by decreased abundance of certain bacterial species reflecting the healthy state rather than by the introduction of pathogens.
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spelling pubmed-60894172018-08-30 Variability of core microbiota in newly diagnosed treatment-naïve paediatric inflammatory bowel disease patients de Meij, T. G. J. de Groot, E. F. J. Peeters, C. F. W. de Boer, N. K. H. Kneepkens, C. M. F. Eck, A. Benninga, M. A. Savelkoul, P. H. M. van Bodegraven, A. A. Budding, A. E. PLoS One Research Article BACKGROUND & AIMS: Intestinal microbiota is considered to play a crucial role in the aetiology of inflammatory bowel disease (IBD). We aimed to describe faecal microbiota composition and dynamics in a large cohort of children with de novo (naïve) IBD, in comparison to healthy paediatric controls (HC). METHODS: In this prospective study, performed at two tertiary centres, faecal samples from newly diagnosed, treatment-naïve paediatric IBD patients were collected prior to bowel cleansing for colonoscopy (t0) and 1, 3 and 6 weeks and 3 months after initiation of therapy. The microbial profiles of Crohn’s disease (CD) and Ulcerative colitis (UC) patients were compared with HC and linked to therapeutic response. Microbiota composition was analysed by IS-pro technology. RESULTS: Microbial profiles of 104 new IBD-patients (63 CD, 41 UC, median age 14.0 years) were compared to 61 HC (median 7.8 years). IBD was mainly characterised by decreased abundance of Alistipes finegoldii and Alistipes putredinis, which characterize a healthy state microbial core. The classifier including these core species as predictors achieved an AUC of the ROC curve of .87. Core bacteria tended to regain abundance during treatment, but did not reach healthy levels. CONCLUSION: Faecal microbiota profiles of children with de novo CD and UC can be discriminated from HC with high accuracy, mainly driven by a decreased abundance of species shaping the microbial core in the healthy state. Paediatric IBD can therefore be characterized by decreased abundance of certain bacterial species reflecting the healthy state rather than by the introduction of pathogens. Public Library of Science 2018-08-13 /pmc/articles/PMC6089417/ /pubmed/30102706 http://dx.doi.org/10.1371/journal.pone.0197649 Text en © 2018 de Meij et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
de Meij, T. G. J.
de Groot, E. F. J.
Peeters, C. F. W.
de Boer, N. K. H.
Kneepkens, C. M. F.
Eck, A.
Benninga, M. A.
Savelkoul, P. H. M.
van Bodegraven, A. A.
Budding, A. E.
Variability of core microbiota in newly diagnosed treatment-naïve paediatric inflammatory bowel disease patients
title Variability of core microbiota in newly diagnosed treatment-naïve paediatric inflammatory bowel disease patients
title_full Variability of core microbiota in newly diagnosed treatment-naïve paediatric inflammatory bowel disease patients
title_fullStr Variability of core microbiota in newly diagnosed treatment-naïve paediatric inflammatory bowel disease patients
title_full_unstemmed Variability of core microbiota in newly diagnosed treatment-naïve paediatric inflammatory bowel disease patients
title_short Variability of core microbiota in newly diagnosed treatment-naïve paediatric inflammatory bowel disease patients
title_sort variability of core microbiota in newly diagnosed treatment-naïve paediatric inflammatory bowel disease patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6089417/
https://www.ncbi.nlm.nih.gov/pubmed/30102706
http://dx.doi.org/10.1371/journal.pone.0197649
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