Cargando…

High-resolution synchrotron-based X-ray microtomography as a tool to unveil the three-dimensional neuronal architecture of the brain

The assessment of neuronal number, spatial organization and connectivity is fundamental for a complete understanding of brain function. However, the evaluation of the three-dimensional (3D) brain cytoarchitecture at cellular resolution persists as a great challenge in the field of neuroscience. In t...

Descripción completa

Detalles Bibliográficos
Autores principales: Fonseca, Matheus de Castro, Araujo, Bruno Henrique Silva, Dias, Carlos Sato Baraldi, Archilha, Nathaly Lopes, Neto, Dionísio Pedro Amorim, Cavalheiro, Esper, Westfahl, Harry, da Silva, Antônio José Roque, Franchini, Kleber Gomes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6089932/
https://www.ncbi.nlm.nih.gov/pubmed/30104676
http://dx.doi.org/10.1038/s41598-018-30501-x
Descripción
Sumario:The assessment of neuronal number, spatial organization and connectivity is fundamental for a complete understanding of brain function. However, the evaluation of the three-dimensional (3D) brain cytoarchitecture at cellular resolution persists as a great challenge in the field of neuroscience. In this context, X-ray microtomography has shown to be a valuable non-destructive tool for imaging a broad range of samples, from dense materials to soft biological specimens, arisen as a new method for deciphering the cytoarchitecture and connectivity of the brain. In this work we present a method for imaging whole neurons in the brain, combining synchrotron-based X-ray microtomography with the Golgi-Cox mercury-based impregnation protocol. In contrast to optical 3D techniques, the approach shown here does neither require tissue slicing or clearing, and allows the investigation of several cells within a 3D region of the brain.