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Protective effects of honokiol against oxidative stress-induced apoptotic signaling in mouse podocytes treated with H2O2
Honokiol (HNK), an important bioactive compound purified from Magnolia officinalis Cortex, has been demonstrated to have manifold beneficial anti-oxidative, anti-inflammatory, anti-bacterial and antitumor pharmacological effects. In the present study, the association of HNK in the signaling mechanis...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6090302/ https://www.ncbi.nlm.nih.gov/pubmed/30116378 http://dx.doi.org/10.3892/etm.2018.6313 |
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author | Wu, Fang Yao, Hangping Zheng, Fenping Tang, Shengjie Lin, Xihua Li, Lin Zhou, Jiaqiang Li, Hong |
author_facet | Wu, Fang Yao, Hangping Zheng, Fenping Tang, Shengjie Lin, Xihua Li, Lin Zhou, Jiaqiang Li, Hong |
author_sort | Wu, Fang |
collection | PubMed |
description | Honokiol (HNK), an important bioactive compound purified from Magnolia officinalis Cortex, has been demonstrated to have manifold beneficial anti-oxidative, anti-inflammatory, anti-bacterial and antitumor pharmacological effects. In the present study, the association of HNK in the signaling mechanism associated with hydrogen peroxide (H(2)O(2))-induced apoptosis of cultured mouse podocytes was investigated. HNK did not cause significant changes in podocyte viability when its concentration remained below 20 µM. MTS assay and flow cytometry confirmed that H(2)O(2) significantly enhanced the rates of apoptosis while produce significant reduction in viability of podocytes. Following 24 h of pre-treatment with different concentrations of HNK, the viability of adherent podocytes increased and apoptosis significantly decreased in a dose-dependent manner below 20 µM. Reverse transcription-polymerase chain reaction and western blot results indicated that HNK significantly decreased the expression of mRNA and cleaved protein of caspase-3 and caspase-9 in podocytes pre-treated with H(2)O(2). Furthermore, phosphorylation of the signaling molecules protein kinase B (Akt) and extracellular signal-regulated kinase (Erk) 1/2 appeared to increase following HNK treatment. In conclusion, HNK largely eliminated the role of promoting podocyte apoptosis in an oxidative stress environment, which was a protective factor on podocytes cultured with H(2)O(2). The anti-oxidative stress mechanisms of HNK are partly due to suppressing the expression of caspase-3 and caspase-9 and upregulating phosphorylated-Akt and -Erk 1/2. |
format | Online Article Text |
id | pubmed-6090302 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-60903022018-08-16 Protective effects of honokiol against oxidative stress-induced apoptotic signaling in mouse podocytes treated with H2O2 Wu, Fang Yao, Hangping Zheng, Fenping Tang, Shengjie Lin, Xihua Li, Lin Zhou, Jiaqiang Li, Hong Exp Ther Med Articles Honokiol (HNK), an important bioactive compound purified from Magnolia officinalis Cortex, has been demonstrated to have manifold beneficial anti-oxidative, anti-inflammatory, anti-bacterial and antitumor pharmacological effects. In the present study, the association of HNK in the signaling mechanism associated with hydrogen peroxide (H(2)O(2))-induced apoptosis of cultured mouse podocytes was investigated. HNK did not cause significant changes in podocyte viability when its concentration remained below 20 µM. MTS assay and flow cytometry confirmed that H(2)O(2) significantly enhanced the rates of apoptosis while produce significant reduction in viability of podocytes. Following 24 h of pre-treatment with different concentrations of HNK, the viability of adherent podocytes increased and apoptosis significantly decreased in a dose-dependent manner below 20 µM. Reverse transcription-polymerase chain reaction and western blot results indicated that HNK significantly decreased the expression of mRNA and cleaved protein of caspase-3 and caspase-9 in podocytes pre-treated with H(2)O(2). Furthermore, phosphorylation of the signaling molecules protein kinase B (Akt) and extracellular signal-regulated kinase (Erk) 1/2 appeared to increase following HNK treatment. In conclusion, HNK largely eliminated the role of promoting podocyte apoptosis in an oxidative stress environment, which was a protective factor on podocytes cultured with H(2)O(2). The anti-oxidative stress mechanisms of HNK are partly due to suppressing the expression of caspase-3 and caspase-9 and upregulating phosphorylated-Akt and -Erk 1/2. D.A. Spandidos 2018-08 2018-06-14 /pmc/articles/PMC6090302/ /pubmed/30116378 http://dx.doi.org/10.3892/etm.2018.6313 Text en Copyright: © Wu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Wu, Fang Yao, Hangping Zheng, Fenping Tang, Shengjie Lin, Xihua Li, Lin Zhou, Jiaqiang Li, Hong Protective effects of honokiol against oxidative stress-induced apoptotic signaling in mouse podocytes treated with H2O2 |
title | Protective effects of honokiol against oxidative stress-induced apoptotic signaling in mouse podocytes treated with H2O2 |
title_full | Protective effects of honokiol against oxidative stress-induced apoptotic signaling in mouse podocytes treated with H2O2 |
title_fullStr | Protective effects of honokiol against oxidative stress-induced apoptotic signaling in mouse podocytes treated with H2O2 |
title_full_unstemmed | Protective effects of honokiol against oxidative stress-induced apoptotic signaling in mouse podocytes treated with H2O2 |
title_short | Protective effects of honokiol against oxidative stress-induced apoptotic signaling in mouse podocytes treated with H2O2 |
title_sort | protective effects of honokiol against oxidative stress-induced apoptotic signaling in mouse podocytes treated with h2o2 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6090302/ https://www.ncbi.nlm.nih.gov/pubmed/30116378 http://dx.doi.org/10.3892/etm.2018.6313 |
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