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Cord blood leptin and insulin levels in association with mitochondrial DNA content
BACKGROUND: The developmental origins of health and disease theory states that a disturbance in the early life environment can contribute to disease risk in later life. Leptin and insulin are anorectic hormones involved in energy homeostasis and are crucial for foetal growth. Disturbances in the lev...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6090601/ https://www.ncbi.nlm.nih.gov/pubmed/30103773 http://dx.doi.org/10.1186/s12967-018-1599-z |
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author | Vriens, Annette Plusquin, Michelle Baeyens, Willy Bruckers, Liesbeth Den Hond, Elly Loots, Ilse Nelen, Vera Schoeters, Greet Janssen, Bram G. Nawrot, Tim S. |
author_facet | Vriens, Annette Plusquin, Michelle Baeyens, Willy Bruckers, Liesbeth Den Hond, Elly Loots, Ilse Nelen, Vera Schoeters, Greet Janssen, Bram G. Nawrot, Tim S. |
author_sort | Vriens, Annette |
collection | PubMed |
description | BACKGROUND: The developmental origins of health and disease theory states that a disturbance in the early life environment can contribute to disease risk in later life. Leptin and insulin are anorectic hormones involved in energy homeostasis and are crucial for foetal growth. Disturbances in the levels of these hormones contribute to obesity and diabetes. In adults, altered mitochondrial function is an important hallmark of metabolic disorders, including obesity and diabetes. However, the mitochondrial effects of early life metabolic variation are unexplored. We investigated whether there is an association between metabolic hormones and mitochondrial DNA (mtDNA) content in early life. METHODS: The study included 236 newborns from the FLEHS III birth cohort, Flanders (Belgium). Relative mtDNA content of cord blood leukocytes was determined using quantitative PCR. Cord blood levels of leptin and insulin were determined using immunoassays. We studied the association between these metabolic hormones and mtDNA content using multiple linear regression models, while accounting for covariates and potential confounders. RESULTS: Leptin and insulin levels were positively associated with cord blood mtDNA content. mtDNA content was respectively 4.49% (95% CI 1.15–7.93; p = 0.008) and 1.60% (95% CI 0.31–2.91; p = 0.02) higher for a interquartile range increase of respectively cord blood leptin and insulin levels. In a sensitivity analysis, we observed that insulin and leptin were independently associated to mtDNA content and that insulin was stronger associated to mtDNA content in boys than in girls. CONCLUSION: Neonatal metabolic hormones were associated with cord blood mtDNA content, which suggests that in early life the variation of mtDNA content might accommodate or reflect changes in the metabolic status. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12967-018-1599-z) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6090601 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-60906012018-08-17 Cord blood leptin and insulin levels in association with mitochondrial DNA content Vriens, Annette Plusquin, Michelle Baeyens, Willy Bruckers, Liesbeth Den Hond, Elly Loots, Ilse Nelen, Vera Schoeters, Greet Janssen, Bram G. Nawrot, Tim S. J Transl Med Research BACKGROUND: The developmental origins of health and disease theory states that a disturbance in the early life environment can contribute to disease risk in later life. Leptin and insulin are anorectic hormones involved in energy homeostasis and are crucial for foetal growth. Disturbances in the levels of these hormones contribute to obesity and diabetes. In adults, altered mitochondrial function is an important hallmark of metabolic disorders, including obesity and diabetes. However, the mitochondrial effects of early life metabolic variation are unexplored. We investigated whether there is an association between metabolic hormones and mitochondrial DNA (mtDNA) content in early life. METHODS: The study included 236 newborns from the FLEHS III birth cohort, Flanders (Belgium). Relative mtDNA content of cord blood leukocytes was determined using quantitative PCR. Cord blood levels of leptin and insulin were determined using immunoassays. We studied the association between these metabolic hormones and mtDNA content using multiple linear regression models, while accounting for covariates and potential confounders. RESULTS: Leptin and insulin levels were positively associated with cord blood mtDNA content. mtDNA content was respectively 4.49% (95% CI 1.15–7.93; p = 0.008) and 1.60% (95% CI 0.31–2.91; p = 0.02) higher for a interquartile range increase of respectively cord blood leptin and insulin levels. In a sensitivity analysis, we observed that insulin and leptin were independently associated to mtDNA content and that insulin was stronger associated to mtDNA content in boys than in girls. CONCLUSION: Neonatal metabolic hormones were associated with cord blood mtDNA content, which suggests that in early life the variation of mtDNA content might accommodate or reflect changes in the metabolic status. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12967-018-1599-z) contains supplementary material, which is available to authorized users. BioMed Central 2018-08-13 /pmc/articles/PMC6090601/ /pubmed/30103773 http://dx.doi.org/10.1186/s12967-018-1599-z Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Vriens, Annette Plusquin, Michelle Baeyens, Willy Bruckers, Liesbeth Den Hond, Elly Loots, Ilse Nelen, Vera Schoeters, Greet Janssen, Bram G. Nawrot, Tim S. Cord blood leptin and insulin levels in association with mitochondrial DNA content |
title | Cord blood leptin and insulin levels in association with mitochondrial DNA content |
title_full | Cord blood leptin and insulin levels in association with mitochondrial DNA content |
title_fullStr | Cord blood leptin and insulin levels in association with mitochondrial DNA content |
title_full_unstemmed | Cord blood leptin and insulin levels in association with mitochondrial DNA content |
title_short | Cord blood leptin and insulin levels in association with mitochondrial DNA content |
title_sort | cord blood leptin and insulin levels in association with mitochondrial dna content |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6090601/ https://www.ncbi.nlm.nih.gov/pubmed/30103773 http://dx.doi.org/10.1186/s12967-018-1599-z |
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