Cargando…

Laboratory evaluation of the efficacy of lotilaner (Credelio™) against Haemaphysalis longicornis infestations of dogs

BACKGROUND: Throughout Japan, Korea and China, Haemaphysalis longicornis ticks are vectors of Babesia gibsoni, which causes severe and progressive anemia in dogs. This study evaluated the efficacy of a single administration of lotilaner flavored chewable tablets (Credelio(TM)) against experimental c...

Descripción completa

Detalles Bibliográficos
Autores principales: Otaki, Hiroshi, Sonobe, Junko, Murphy, Martin, Cavalleri, Daniela, Seewald, Wolfgang, Drake, Jason, Nanchen, Steve
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6090816/
https://www.ncbi.nlm.nih.gov/pubmed/30071885
http://dx.doi.org/10.1186/s13071-018-3032-0
_version_ 1783347266193457152
author Otaki, Hiroshi
Sonobe, Junko
Murphy, Martin
Cavalleri, Daniela
Seewald, Wolfgang
Drake, Jason
Nanchen, Steve
author_facet Otaki, Hiroshi
Sonobe, Junko
Murphy, Martin
Cavalleri, Daniela
Seewald, Wolfgang
Drake, Jason
Nanchen, Steve
author_sort Otaki, Hiroshi
collection PubMed
description BACKGROUND: Throughout Japan, Korea and China, Haemaphysalis longicornis ticks are vectors of Babesia gibsoni, which causes severe and progressive anemia in dogs. This study evaluated the efficacy of a single administration of lotilaner flavored chewable tablets (Credelio(TM)) against experimental canine H. longicornis infestations. METHODS: Twenty-two healthy Beagles were ranked in descending order of counts of H. longicornis completed 48 h after challenge on Day -7. The 16 dogs with the highest live tick counts were blocked into pairs and within pairs randomized to either lotilaner-treatment at a minimum dose rate of 20 mg/kg or sham-treated controls. Treatment was administered within 30 ± 5 min following feeding on Day 0. Infestations with 50 unfed adult H. longicornis were completed on Days -2, 7, 14, 21, 28 and 35. Elizabethan collars were placed for 48 (± 2) h after each infestation and a T-shirt was placed on each dog to facilitate attachment. Ticks were counted in situ 12 and 24 h post-treatment and counted and removed after an additional 24 h (48 h after treatment) and 48 h after each post-treatment infestation. Dogs were sedated for tick challenges and counts. Live attached ticks on each dog were counted for efficacy assessments. Lotilaner was considered effective if the average tick attachment rate in the control group was at least 20%, if there was a statistically significant difference (P < 0.05) in mean tick counts between treated and control groups, and if the lotilaner-treated group had a calculated efficacy of at least 90%. RESULTS: Average control group retention of the H. longicornis challenge exceeded 20% at each assessment. Lotilaner started killing H. longicornis ticks quickly, achieving 57.4% efficacy within 12 h. At 48 h post-treatment, and following each subsequent infestation, between-group differences in mean H. longicornis counts were significant (P < 0.0001). From 48 h post-treatment, through the final assessment on Day 37, lotilaner efficacy remained greater than 95%, including on Day 37 when efficacy was 98.4%. CONCLUSION: Lotilaner, administered to dogs orally at a minimum dose rate of 20 mg/kg is well tolerated, provides rapid reduction of existing H. longicornis tick infestations, and provides sustained residual protection for at least 35 days.
format Online
Article
Text
id pubmed-6090816
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-60908162018-08-17 Laboratory evaluation of the efficacy of lotilaner (Credelio™) against Haemaphysalis longicornis infestations of dogs Otaki, Hiroshi Sonobe, Junko Murphy, Martin Cavalleri, Daniela Seewald, Wolfgang Drake, Jason Nanchen, Steve Parasit Vectors Research BACKGROUND: Throughout Japan, Korea and China, Haemaphysalis longicornis ticks are vectors of Babesia gibsoni, which causes severe and progressive anemia in dogs. This study evaluated the efficacy of a single administration of lotilaner flavored chewable tablets (Credelio(TM)) against experimental canine H. longicornis infestations. METHODS: Twenty-two healthy Beagles were ranked in descending order of counts of H. longicornis completed 48 h after challenge on Day -7. The 16 dogs with the highest live tick counts were blocked into pairs and within pairs randomized to either lotilaner-treatment at a minimum dose rate of 20 mg/kg or sham-treated controls. Treatment was administered within 30 ± 5 min following feeding on Day 0. Infestations with 50 unfed adult H. longicornis were completed on Days -2, 7, 14, 21, 28 and 35. Elizabethan collars were placed for 48 (± 2) h after each infestation and a T-shirt was placed on each dog to facilitate attachment. Ticks were counted in situ 12 and 24 h post-treatment and counted and removed after an additional 24 h (48 h after treatment) and 48 h after each post-treatment infestation. Dogs were sedated for tick challenges and counts. Live attached ticks on each dog were counted for efficacy assessments. Lotilaner was considered effective if the average tick attachment rate in the control group was at least 20%, if there was a statistically significant difference (P < 0.05) in mean tick counts between treated and control groups, and if the lotilaner-treated group had a calculated efficacy of at least 90%. RESULTS: Average control group retention of the H. longicornis challenge exceeded 20% at each assessment. Lotilaner started killing H. longicornis ticks quickly, achieving 57.4% efficacy within 12 h. At 48 h post-treatment, and following each subsequent infestation, between-group differences in mean H. longicornis counts were significant (P < 0.0001). From 48 h post-treatment, through the final assessment on Day 37, lotilaner efficacy remained greater than 95%, including on Day 37 when efficacy was 98.4%. CONCLUSION: Lotilaner, administered to dogs orally at a minimum dose rate of 20 mg/kg is well tolerated, provides rapid reduction of existing H. longicornis tick infestations, and provides sustained residual protection for at least 35 days. BioMed Central 2018-08-02 /pmc/articles/PMC6090816/ /pubmed/30071885 http://dx.doi.org/10.1186/s13071-018-3032-0 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Otaki, Hiroshi
Sonobe, Junko
Murphy, Martin
Cavalleri, Daniela
Seewald, Wolfgang
Drake, Jason
Nanchen, Steve
Laboratory evaluation of the efficacy of lotilaner (Credelio™) against Haemaphysalis longicornis infestations of dogs
title Laboratory evaluation of the efficacy of lotilaner (Credelio™) against Haemaphysalis longicornis infestations of dogs
title_full Laboratory evaluation of the efficacy of lotilaner (Credelio™) against Haemaphysalis longicornis infestations of dogs
title_fullStr Laboratory evaluation of the efficacy of lotilaner (Credelio™) against Haemaphysalis longicornis infestations of dogs
title_full_unstemmed Laboratory evaluation of the efficacy of lotilaner (Credelio™) against Haemaphysalis longicornis infestations of dogs
title_short Laboratory evaluation of the efficacy of lotilaner (Credelio™) against Haemaphysalis longicornis infestations of dogs
title_sort laboratory evaluation of the efficacy of lotilaner (credelio™) against haemaphysalis longicornis infestations of dogs
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6090816/
https://www.ncbi.nlm.nih.gov/pubmed/30071885
http://dx.doi.org/10.1186/s13071-018-3032-0
work_keys_str_mv AT otakihiroshi laboratoryevaluationoftheefficacyoflotilanercredelioagainsthaemaphysalislongicornisinfestationsofdogs
AT sonobejunko laboratoryevaluationoftheefficacyoflotilanercredelioagainsthaemaphysalislongicornisinfestationsofdogs
AT murphymartin laboratoryevaluationoftheefficacyoflotilanercredelioagainsthaemaphysalislongicornisinfestationsofdogs
AT cavalleridaniela laboratoryevaluationoftheefficacyoflotilanercredelioagainsthaemaphysalislongicornisinfestationsofdogs
AT seewaldwolfgang laboratoryevaluationoftheefficacyoflotilanercredelioagainsthaemaphysalislongicornisinfestationsofdogs
AT drakejason laboratoryevaluationoftheefficacyoflotilanercredelioagainsthaemaphysalislongicornisinfestationsofdogs
AT nanchensteve laboratoryevaluationoftheefficacyoflotilanercredelioagainsthaemaphysalislongicornisinfestationsofdogs