In vivo formaldehyde cross-linking: it is time for black box analysis

Formaldehyde cross-linking is an important component of many technologies, including chromatin immunoprecipitation and chromosome conformation capture. The procedure remains empirical and poorly characterized, however, despite a long history of its use in research. Little is known about the specific...

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Detalles Bibliográficos
Autores principales: Gavrilov, Alexey, Razin, Sergey V., Cavalli, Giacomo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Letters to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6090872/
https://www.ncbi.nlm.nih.gov/pubmed/25241225
http://dx.doi.org/10.1093/bfgp/elu037
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author Gavrilov, Alexey
Razin, Sergey V.
Cavalli, Giacomo
author_facet Gavrilov, Alexey
Razin, Sergey V.
Cavalli, Giacomo
author_sort Gavrilov, Alexey
collection PubMed
description Formaldehyde cross-linking is an important component of many technologies, including chromatin immunoprecipitation and chromosome conformation capture. The procedure remains empirical and poorly characterized, however, despite a long history of its use in research. Little is known about the specificity of in vivo cross-linking, its efficiency and chemical adducts induced by the procedure. It is time to search this black box.
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spelling pubmed-60908722018-08-17 In vivo formaldehyde cross-linking: it is time for black box analysis Gavrilov, Alexey Razin, Sergey V. Cavalli, Giacomo Brief Funct Genomics Letters to the Editor Formaldehyde cross-linking is an important component of many technologies, including chromatin immunoprecipitation and chromosome conformation capture. The procedure remains empirical and poorly characterized, however, despite a long history of its use in research. Little is known about the specificity of in vivo cross-linking, its efficiency and chemical adducts induced by the procedure. It is time to search this black box. Oxford University Press 2015-03 2014-09-19 /pmc/articles/PMC6090872/ /pubmed/25241225 http://dx.doi.org/10.1093/bfgp/elu037 Text en © The Author 2014. Published by Oxford University Press. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Letters to the Editor
Gavrilov, Alexey
Razin, Sergey V.
Cavalli, Giacomo
In vivo formaldehyde cross-linking: it is time for black box analysis
title In vivo formaldehyde cross-linking: it is time for black box analysis
title_full In vivo formaldehyde cross-linking: it is time for black box analysis
title_fullStr In vivo formaldehyde cross-linking: it is time for black box analysis
title_full_unstemmed In vivo formaldehyde cross-linking: it is time for black box analysis
title_short In vivo formaldehyde cross-linking: it is time for black box analysis
title_sort in vivo formaldehyde cross-linking: it is time for black box analysis
topic Letters to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6090872/
https://www.ncbi.nlm.nih.gov/pubmed/25241225
http://dx.doi.org/10.1093/bfgp/elu037
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