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Overexpression of the Kininogen-1 inhibits proliferation and induces apoptosis of glioma cells

BACKGROUND: Glioma is the most common primary central nervous system tumor derived from glial cells. Kininogen-1 (KNG1) can exert antiangiogenic properties and inhibit proliferation of endothelial cells. The effect of KNG1 on the glioma is rarely reported, so our purpose in to explore its mechanism...

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Autores principales: Xu, Jinfang, Fang, Jun, Cheng, Zhonghao, Fan, Longlong, Hu, Weiwei, Zhou, Feng, Shen, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6090912/
https://www.ncbi.nlm.nih.gov/pubmed/30068373
http://dx.doi.org/10.1186/s13046-018-0833-0
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author Xu, Jinfang
Fang, Jun
Cheng, Zhonghao
Fan, Longlong
Hu, Weiwei
Zhou, Feng
Shen, Hong
author_facet Xu, Jinfang
Fang, Jun
Cheng, Zhonghao
Fan, Longlong
Hu, Weiwei
Zhou, Feng
Shen, Hong
author_sort Xu, Jinfang
collection PubMed
description BACKGROUND: Glioma is the most common primary central nervous system tumor derived from glial cells. Kininogen-1 (KNG1) can exert antiangiogenic properties and inhibit proliferation of endothelial cells. The effect of KNG1 on the glioma is rarely reported, so our purpose in to explore its mechanism in glioma cells. METHODS: The differentially expressed genes (DEGs) were identified based on The Cancer Genome Atlas (TCGA) database. The KNG1-vector was transfected into the two glioma cells. The viability, apoptosis and cell cycle of glioma cells and microvessel density (MVD) were detected by cell counting kit-8 assay, flow cytometry and immunohistochemistry, respectively. The expression were measured by quantitative real-time PCR and Western blot, respectively. A tumor mouse model was established to determine apoptosis rate of brain tissue by terminal deoxynucleotidyl transfer-mediated dUTP nick end labeling (TUNEL) analysis. RESULTS: KNG1 was identified as the core gene and lowly expressed in the glioma cells. Overexpression of KNG1 inhibited cell viability and angiogenesis of glioma cells. Overexpression of KNG1 promoted the apoptosis and G1 phase cell cycle arrest of glioma cells. Moreover, the expressions of VEGF, cyclinD1, ki67, caspase-3/9 and XIAP were regulated by overexpression of KNG1. In addition, overexpression of KNG1 inhibited the activity of PI3K/Akt. Furthermore, overexpression of KNG1 decreased the tumor growth and promoted the apoptosis of decreased by overexpression of KNG1 in vivo. . CONCLUSIONS: Overexpression of KNG1 suppresses glioma progression by inhibiting the proliferation and promoting apoptosis of glioma cells, providing a therapeutic strategy for the malignant glioma.
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spelling pubmed-60909122018-08-17 Overexpression of the Kininogen-1 inhibits proliferation and induces apoptosis of glioma cells Xu, Jinfang Fang, Jun Cheng, Zhonghao Fan, Longlong Hu, Weiwei Zhou, Feng Shen, Hong J Exp Clin Cancer Res Research BACKGROUND: Glioma is the most common primary central nervous system tumor derived from glial cells. Kininogen-1 (KNG1) can exert antiangiogenic properties and inhibit proliferation of endothelial cells. The effect of KNG1 on the glioma is rarely reported, so our purpose in to explore its mechanism in glioma cells. METHODS: The differentially expressed genes (DEGs) were identified based on The Cancer Genome Atlas (TCGA) database. The KNG1-vector was transfected into the two glioma cells. The viability, apoptosis and cell cycle of glioma cells and microvessel density (MVD) were detected by cell counting kit-8 assay, flow cytometry and immunohistochemistry, respectively. The expression were measured by quantitative real-time PCR and Western blot, respectively. A tumor mouse model was established to determine apoptosis rate of brain tissue by terminal deoxynucleotidyl transfer-mediated dUTP nick end labeling (TUNEL) analysis. RESULTS: KNG1 was identified as the core gene and lowly expressed in the glioma cells. Overexpression of KNG1 inhibited cell viability and angiogenesis of glioma cells. Overexpression of KNG1 promoted the apoptosis and G1 phase cell cycle arrest of glioma cells. Moreover, the expressions of VEGF, cyclinD1, ki67, caspase-3/9 and XIAP were regulated by overexpression of KNG1. In addition, overexpression of KNG1 inhibited the activity of PI3K/Akt. Furthermore, overexpression of KNG1 decreased the tumor growth and promoted the apoptosis of decreased by overexpression of KNG1 in vivo. . CONCLUSIONS: Overexpression of KNG1 suppresses glioma progression by inhibiting the proliferation and promoting apoptosis of glioma cells, providing a therapeutic strategy for the malignant glioma. BioMed Central 2018-08-02 /pmc/articles/PMC6090912/ /pubmed/30068373 http://dx.doi.org/10.1186/s13046-018-0833-0 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Xu, Jinfang
Fang, Jun
Cheng, Zhonghao
Fan, Longlong
Hu, Weiwei
Zhou, Feng
Shen, Hong
Overexpression of the Kininogen-1 inhibits proliferation and induces apoptosis of glioma cells
title Overexpression of the Kininogen-1 inhibits proliferation and induces apoptosis of glioma cells
title_full Overexpression of the Kininogen-1 inhibits proliferation and induces apoptosis of glioma cells
title_fullStr Overexpression of the Kininogen-1 inhibits proliferation and induces apoptosis of glioma cells
title_full_unstemmed Overexpression of the Kininogen-1 inhibits proliferation and induces apoptosis of glioma cells
title_short Overexpression of the Kininogen-1 inhibits proliferation and induces apoptosis of glioma cells
title_sort overexpression of the kininogen-1 inhibits proliferation and induces apoptosis of glioma cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6090912/
https://www.ncbi.nlm.nih.gov/pubmed/30068373
http://dx.doi.org/10.1186/s13046-018-0833-0
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