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Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids

Cultured mesenchymal stromal cells (MSCs) are cells that can be used for tissue engineering or cell therapies owing to their multipotency and ability to secrete immunomodulatory and trophic molecules. Several studies suggest that MSCs can become pericytes when cocultured with endothelial cells (ECs)...

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Autores principales: Bellagamba, Bruno Corrêa, Grudzinski, Patrícia Bencke, Ely, Pedro Bins, Nader, Paulo de Jesus Hartmann, Nardi, Nance Beyer, da Silva Meirelles, Lindolfo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6091361/
https://www.ncbi.nlm.nih.gov/pubmed/30154865
http://dx.doi.org/10.1155/2018/7357213
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author Bellagamba, Bruno Corrêa
Grudzinski, Patrícia Bencke
Ely, Pedro Bins
Nader, Paulo de Jesus Hartmann
Nardi, Nance Beyer
da Silva Meirelles, Lindolfo
author_facet Bellagamba, Bruno Corrêa
Grudzinski, Patrícia Bencke
Ely, Pedro Bins
Nader, Paulo de Jesus Hartmann
Nardi, Nance Beyer
da Silva Meirelles, Lindolfo
author_sort Bellagamba, Bruno Corrêa
collection PubMed
description Cultured mesenchymal stromal cells (MSCs) are cells that can be used for tissue engineering or cell therapies owing to their multipotency and ability to secrete immunomodulatory and trophic molecules. Several studies suggest that MSCs can become pericytes when cocultured with endothelial cells (ECs) but failed to use pericyte markers not already expressed by MSCs. We hypothesized ECs could instruct MSCs to express the molecules CD271 or CD34, which are expressed by pericytes in situ but not by MSCs. CD271 is a marker of especial interest because it is associated with multipotency, a characteristic that wanes in MSCs as they are culture expanded. Consequently, surface expression of CD271 and CD34 was detected in roughly half of the MSCs cocultured with ECs as spheroids in the presence of insulin-like growth factor 1 (IGF-1). Conversely, expression of CD271 and CD34 was detected in a similar proportion of MSCs cultured under these conditions without ECs, and expression of these markers was low or absent when no IGF-1 was added. These findings indicate that specific culture conditions including IGF-1 can endow cultured MSCs with expression of CD271 and CD34, which may enhance the multipotency of these cells when they are used for therapeutic purposes.
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spelling pubmed-60913612018-08-28 Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids Bellagamba, Bruno Corrêa Grudzinski, Patrícia Bencke Ely, Pedro Bins Nader, Paulo de Jesus Hartmann Nardi, Nance Beyer da Silva Meirelles, Lindolfo Stem Cells Int Research Article Cultured mesenchymal stromal cells (MSCs) are cells that can be used for tissue engineering or cell therapies owing to their multipotency and ability to secrete immunomodulatory and trophic molecules. Several studies suggest that MSCs can become pericytes when cocultured with endothelial cells (ECs) but failed to use pericyte markers not already expressed by MSCs. We hypothesized ECs could instruct MSCs to express the molecules CD271 or CD34, which are expressed by pericytes in situ but not by MSCs. CD271 is a marker of especial interest because it is associated with multipotency, a characteristic that wanes in MSCs as they are culture expanded. Consequently, surface expression of CD271 and CD34 was detected in roughly half of the MSCs cocultured with ECs as spheroids in the presence of insulin-like growth factor 1 (IGF-1). Conversely, expression of CD271 and CD34 was detected in a similar proportion of MSCs cultured under these conditions without ECs, and expression of these markers was low or absent when no IGF-1 was added. These findings indicate that specific culture conditions including IGF-1 can endow cultured MSCs with expression of CD271 and CD34, which may enhance the multipotency of these cells when they are used for therapeutic purposes. Hindawi 2018-07-31 /pmc/articles/PMC6091361/ /pubmed/30154865 http://dx.doi.org/10.1155/2018/7357213 Text en Copyright © 2018 Bruno Corrêa Bellagamba et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bellagamba, Bruno Corrêa
Grudzinski, Patrícia Bencke
Ely, Pedro Bins
Nader, Paulo de Jesus Hartmann
Nardi, Nance Beyer
da Silva Meirelles, Lindolfo
Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids
title Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids
title_full Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids
title_fullStr Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids
title_full_unstemmed Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids
title_short Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids
title_sort induction of expression of cd271 and cd34 in mesenchymal stromal cells cultured as spheroids
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6091361/
https://www.ncbi.nlm.nih.gov/pubmed/30154865
http://dx.doi.org/10.1155/2018/7357213
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