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Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids
Cultured mesenchymal stromal cells (MSCs) are cells that can be used for tissue engineering or cell therapies owing to their multipotency and ability to secrete immunomodulatory and trophic molecules. Several studies suggest that MSCs can become pericytes when cocultured with endothelial cells (ECs)...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6091361/ https://www.ncbi.nlm.nih.gov/pubmed/30154865 http://dx.doi.org/10.1155/2018/7357213 |
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author | Bellagamba, Bruno Corrêa Grudzinski, Patrícia Bencke Ely, Pedro Bins Nader, Paulo de Jesus Hartmann Nardi, Nance Beyer da Silva Meirelles, Lindolfo |
author_facet | Bellagamba, Bruno Corrêa Grudzinski, Patrícia Bencke Ely, Pedro Bins Nader, Paulo de Jesus Hartmann Nardi, Nance Beyer da Silva Meirelles, Lindolfo |
author_sort | Bellagamba, Bruno Corrêa |
collection | PubMed |
description | Cultured mesenchymal stromal cells (MSCs) are cells that can be used for tissue engineering or cell therapies owing to their multipotency and ability to secrete immunomodulatory and trophic molecules. Several studies suggest that MSCs can become pericytes when cocultured with endothelial cells (ECs) but failed to use pericyte markers not already expressed by MSCs. We hypothesized ECs could instruct MSCs to express the molecules CD271 or CD34, which are expressed by pericytes in situ but not by MSCs. CD271 is a marker of especial interest because it is associated with multipotency, a characteristic that wanes in MSCs as they are culture expanded. Consequently, surface expression of CD271 and CD34 was detected in roughly half of the MSCs cocultured with ECs as spheroids in the presence of insulin-like growth factor 1 (IGF-1). Conversely, expression of CD271 and CD34 was detected in a similar proportion of MSCs cultured under these conditions without ECs, and expression of these markers was low or absent when no IGF-1 was added. These findings indicate that specific culture conditions including IGF-1 can endow cultured MSCs with expression of CD271 and CD34, which may enhance the multipotency of these cells when they are used for therapeutic purposes. |
format | Online Article Text |
id | pubmed-6091361 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-60913612018-08-28 Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids Bellagamba, Bruno Corrêa Grudzinski, Patrícia Bencke Ely, Pedro Bins Nader, Paulo de Jesus Hartmann Nardi, Nance Beyer da Silva Meirelles, Lindolfo Stem Cells Int Research Article Cultured mesenchymal stromal cells (MSCs) are cells that can be used for tissue engineering or cell therapies owing to their multipotency and ability to secrete immunomodulatory and trophic molecules. Several studies suggest that MSCs can become pericytes when cocultured with endothelial cells (ECs) but failed to use pericyte markers not already expressed by MSCs. We hypothesized ECs could instruct MSCs to express the molecules CD271 or CD34, which are expressed by pericytes in situ but not by MSCs. CD271 is a marker of especial interest because it is associated with multipotency, a characteristic that wanes in MSCs as they are culture expanded. Consequently, surface expression of CD271 and CD34 was detected in roughly half of the MSCs cocultured with ECs as spheroids in the presence of insulin-like growth factor 1 (IGF-1). Conversely, expression of CD271 and CD34 was detected in a similar proportion of MSCs cultured under these conditions without ECs, and expression of these markers was low or absent when no IGF-1 was added. These findings indicate that specific culture conditions including IGF-1 can endow cultured MSCs with expression of CD271 and CD34, which may enhance the multipotency of these cells when they are used for therapeutic purposes. Hindawi 2018-07-31 /pmc/articles/PMC6091361/ /pubmed/30154865 http://dx.doi.org/10.1155/2018/7357213 Text en Copyright © 2018 Bruno Corrêa Bellagamba et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Bellagamba, Bruno Corrêa Grudzinski, Patrícia Bencke Ely, Pedro Bins Nader, Paulo de Jesus Hartmann Nardi, Nance Beyer da Silva Meirelles, Lindolfo Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids |
title | Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids |
title_full | Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids |
title_fullStr | Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids |
title_full_unstemmed | Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids |
title_short | Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids |
title_sort | induction of expression of cd271 and cd34 in mesenchymal stromal cells cultured as spheroids |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6091361/ https://www.ncbi.nlm.nih.gov/pubmed/30154865 http://dx.doi.org/10.1155/2018/7357213 |
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