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miR-497 inhibited proliferation, migration and invasion of thyroid papillary carcinoma cells by negatively regulating YAP1 expression
PURPOSE: This article aimed to investigate the effect of miR-497 on thyroid papillary carcinoma. MATERIALS AND METHODS: miR-497 expression was analyzed using The Cancer Genome Atlas. A total of 56 papillary thyroid carcinoma patients’ tumor tissues and normal tissues were collected. Nthy-ori 3-1 and...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Dove Medical Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6091470/ https://www.ncbi.nlm.nih.gov/pubmed/30127619 http://dx.doi.org/10.2147/OTT.S164052 |
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author | Cheng, Hui Dong, Hui Feng, Jianlin Tian, Hongyan Zhang, Haixia Xu, Lina |
author_facet | Cheng, Hui Dong, Hui Feng, Jianlin Tian, Hongyan Zhang, Haixia Xu, Lina |
author_sort | Cheng, Hui |
collection | PubMed |
description | PURPOSE: This article aimed to investigate the effect of miR-497 on thyroid papillary carcinoma. MATERIALS AND METHODS: miR-497 expression was analyzed using The Cancer Genome Atlas. A total of 56 papillary thyroid carcinoma patients’ tumor tissues and normal tissues were collected. Nthy-ori 3-1 and K1 cells were cultured. K1 cells were also transfected. Quantitative real-time polymerase chain reaction and Western blot were used to detect miR-497 and yes-associated protein 1 (YAP1) expression. Luciferase reporter assay was performed. MTT and Transwell assay were conducted to measure cells’ proliferation, migration and invasion. Immunofluorescence detection was used to detect YAP1-positive cells. RESULTS: miR-497 was downregulated, while YAP1 was upregulated in thyroid papillary carcinoma tissues and K1 cells (P<0.05). Compared with the negative control group, the OD495 value and the migrating and invasive cell number were significantly lower in miR-497 mimics group and significantly higher in miR-497 inhibitor group (P<0.05). YAP1 was the target gene of miR-497. Compared with blank group, the OD495 value and the migrating and invasive cell number were significantly lower in si-YAP1 group and significantly higher in miR-497 inhibitor group (P<0.05), while no significant difference was found between si-YAP1+inhibitors group and blank group in these indicators. CONCLUSION: miR-497 regulated the proliferation, migration and invasion of K1 cells by negatively regulating YAP1 expression. |
format | Online Article Text |
id | pubmed-6091470 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-60914702018-08-20 miR-497 inhibited proliferation, migration and invasion of thyroid papillary carcinoma cells by negatively regulating YAP1 expression Cheng, Hui Dong, Hui Feng, Jianlin Tian, Hongyan Zhang, Haixia Xu, Lina Onco Targets Ther Original Research PURPOSE: This article aimed to investigate the effect of miR-497 on thyroid papillary carcinoma. MATERIALS AND METHODS: miR-497 expression was analyzed using The Cancer Genome Atlas. A total of 56 papillary thyroid carcinoma patients’ tumor tissues and normal tissues were collected. Nthy-ori 3-1 and K1 cells were cultured. K1 cells were also transfected. Quantitative real-time polymerase chain reaction and Western blot were used to detect miR-497 and yes-associated protein 1 (YAP1) expression. Luciferase reporter assay was performed. MTT and Transwell assay were conducted to measure cells’ proliferation, migration and invasion. Immunofluorescence detection was used to detect YAP1-positive cells. RESULTS: miR-497 was downregulated, while YAP1 was upregulated in thyroid papillary carcinoma tissues and K1 cells (P<0.05). Compared with the negative control group, the OD495 value and the migrating and invasive cell number were significantly lower in miR-497 mimics group and significantly higher in miR-497 inhibitor group (P<0.05). YAP1 was the target gene of miR-497. Compared with blank group, the OD495 value and the migrating and invasive cell number were significantly lower in si-YAP1 group and significantly higher in miR-497 inhibitor group (P<0.05), while no significant difference was found between si-YAP1+inhibitors group and blank group in these indicators. CONCLUSION: miR-497 regulated the proliferation, migration and invasion of K1 cells by negatively regulating YAP1 expression. Dove Medical Press 2018-08-10 /pmc/articles/PMC6091470/ /pubmed/30127619 http://dx.doi.org/10.2147/OTT.S164052 Text en © 2018 Cheng et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Cheng, Hui Dong, Hui Feng, Jianlin Tian, Hongyan Zhang, Haixia Xu, Lina miR-497 inhibited proliferation, migration and invasion of thyroid papillary carcinoma cells by negatively regulating YAP1 expression |
title | miR-497 inhibited proliferation, migration and invasion of thyroid papillary carcinoma cells by negatively regulating YAP1 expression |
title_full | miR-497 inhibited proliferation, migration and invasion of thyroid papillary carcinoma cells by negatively regulating YAP1 expression |
title_fullStr | miR-497 inhibited proliferation, migration and invasion of thyroid papillary carcinoma cells by negatively regulating YAP1 expression |
title_full_unstemmed | miR-497 inhibited proliferation, migration and invasion of thyroid papillary carcinoma cells by negatively regulating YAP1 expression |
title_short | miR-497 inhibited proliferation, migration and invasion of thyroid papillary carcinoma cells by negatively regulating YAP1 expression |
title_sort | mir-497 inhibited proliferation, migration and invasion of thyroid papillary carcinoma cells by negatively regulating yap1 expression |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6091470/ https://www.ncbi.nlm.nih.gov/pubmed/30127619 http://dx.doi.org/10.2147/OTT.S164052 |
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