Cargando…

Increasing the Resistance of Living Cells against Oxidative Stress by Nonnatural Surfactants as Membrane Guards

[Image: see text] The importation of construction principles or even constituents from biology into materials science is a prevailing concept. Vice versa, the cellular level modification of living systems with nonnatural components is much more difficult to achieve. It has been done for analytical p...

Descripción completa

Detalles Bibliográficos
Autores principales: Kunkel, Marius, Schildknecht, Stefan, Boldt, Klaus, Zeyffert, Lukas, Schleheck, David, Leist, Marcel, Polarz, Sebastian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6091502/
https://www.ncbi.nlm.nih.gov/pubmed/29949339
http://dx.doi.org/10.1021/acsami.8b07032
_version_ 1783347394696445952
author Kunkel, Marius
Schildknecht, Stefan
Boldt, Klaus
Zeyffert, Lukas
Schleheck, David
Leist, Marcel
Polarz, Sebastian
author_facet Kunkel, Marius
Schildknecht, Stefan
Boldt, Klaus
Zeyffert, Lukas
Schleheck, David
Leist, Marcel
Polarz, Sebastian
author_sort Kunkel, Marius
collection PubMed
description [Image: see text] The importation of construction principles or even constituents from biology into materials science is a prevailing concept. Vice versa, the cellular level modification of living systems with nonnatural components is much more difficult to achieve. It has been done for analytical purposes, for example, imaging, to learn something about intracellular processes. Cases describing the improvement of a biological function by the integration of a nonnatural (nano)constituent are extremely rare. Because biological membranes contain some kind of a surfactant, for example, phospholipids, our idea is to modify cells with a newly synthesized surfactant. However, this surfactant is intended to possess an additional functionality, which is the reduction of oxidative stress. We report the synthesis of a surfactant with Janus-type head group architecture, a fullerene C(60) modified by five alkyl chains on one side and an average of 20 oxygen species on the other hemisphere. It is demonstrated that the amphiphilic properties of the fullerenol surfactant are similar to that of lipids. Not only quenching of reactive oxygen species (superoxide, hydroxyl radicals, peroxynitrite, and hydrogen peroxide) was successful, but also the fullerenol surfactant exceeds benchmark antioxidant agents such as quercetin. The surfactant was then brought into contact with different cell types, and the viability even of delicate cells such as human liver cells (HepG2) and human dopaminergic neurons (LUHMES) has proven to be extraordinarily high. We could show further that the cells take up the fullerenol surfactant, and as a consequence, they are protected much better against oxidative stress.
format Online
Article
Text
id pubmed-6091502
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-60915022019-06-27 Increasing the Resistance of Living Cells against Oxidative Stress by Nonnatural Surfactants as Membrane Guards Kunkel, Marius Schildknecht, Stefan Boldt, Klaus Zeyffert, Lukas Schleheck, David Leist, Marcel Polarz, Sebastian ACS Appl Mater Interfaces [Image: see text] The importation of construction principles or even constituents from biology into materials science is a prevailing concept. Vice versa, the cellular level modification of living systems with nonnatural components is much more difficult to achieve. It has been done for analytical purposes, for example, imaging, to learn something about intracellular processes. Cases describing the improvement of a biological function by the integration of a nonnatural (nano)constituent are extremely rare. Because biological membranes contain some kind of a surfactant, for example, phospholipids, our idea is to modify cells with a newly synthesized surfactant. However, this surfactant is intended to possess an additional functionality, which is the reduction of oxidative stress. We report the synthesis of a surfactant with Janus-type head group architecture, a fullerene C(60) modified by five alkyl chains on one side and an average of 20 oxygen species on the other hemisphere. It is demonstrated that the amphiphilic properties of the fullerenol surfactant are similar to that of lipids. Not only quenching of reactive oxygen species (superoxide, hydroxyl radicals, peroxynitrite, and hydrogen peroxide) was successful, but also the fullerenol surfactant exceeds benchmark antioxidant agents such as quercetin. The surfactant was then brought into contact with different cell types, and the viability even of delicate cells such as human liver cells (HepG2) and human dopaminergic neurons (LUHMES) has proven to be extraordinarily high. We could show further that the cells take up the fullerenol surfactant, and as a consequence, they are protected much better against oxidative stress. American Chemical Society 2018-06-27 2018-07-18 /pmc/articles/PMC6091502/ /pubmed/29949339 http://dx.doi.org/10.1021/acsami.8b07032 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Kunkel, Marius
Schildknecht, Stefan
Boldt, Klaus
Zeyffert, Lukas
Schleheck, David
Leist, Marcel
Polarz, Sebastian
Increasing the Resistance of Living Cells against Oxidative Stress by Nonnatural Surfactants as Membrane Guards
title Increasing the Resistance of Living Cells against Oxidative Stress by Nonnatural Surfactants as Membrane Guards
title_full Increasing the Resistance of Living Cells against Oxidative Stress by Nonnatural Surfactants as Membrane Guards
title_fullStr Increasing the Resistance of Living Cells against Oxidative Stress by Nonnatural Surfactants as Membrane Guards
title_full_unstemmed Increasing the Resistance of Living Cells against Oxidative Stress by Nonnatural Surfactants as Membrane Guards
title_short Increasing the Resistance of Living Cells against Oxidative Stress by Nonnatural Surfactants as Membrane Guards
title_sort increasing the resistance of living cells against oxidative stress by nonnatural surfactants as membrane guards
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6091502/
https://www.ncbi.nlm.nih.gov/pubmed/29949339
http://dx.doi.org/10.1021/acsami.8b07032
work_keys_str_mv AT kunkelmarius increasingtheresistanceoflivingcellsagainstoxidativestressbynonnaturalsurfactantsasmembraneguards
AT schildknechtstefan increasingtheresistanceoflivingcellsagainstoxidativestressbynonnaturalsurfactantsasmembraneguards
AT boldtklaus increasingtheresistanceoflivingcellsagainstoxidativestressbynonnaturalsurfactantsasmembraneguards
AT zeyffertlukas increasingtheresistanceoflivingcellsagainstoxidativestressbynonnaturalsurfactantsasmembraneguards
AT schleheckdavid increasingtheresistanceoflivingcellsagainstoxidativestressbynonnaturalsurfactantsasmembraneguards
AT leistmarcel increasingtheresistanceoflivingcellsagainstoxidativestressbynonnaturalsurfactantsasmembraneguards
AT polarzsebastian increasingtheresistanceoflivingcellsagainstoxidativestressbynonnaturalsurfactantsasmembraneguards