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Increasing the Resistance of Living Cells against Oxidative Stress by Nonnatural Surfactants as Membrane Guards
[Image: see text] The importation of construction principles or even constituents from biology into materials science is a prevailing concept. Vice versa, the cellular level modification of living systems with nonnatural components is much more difficult to achieve. It has been done for analytical p...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American
Chemical Society
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6091502/ https://www.ncbi.nlm.nih.gov/pubmed/29949339 http://dx.doi.org/10.1021/acsami.8b07032 |
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author | Kunkel, Marius Schildknecht, Stefan Boldt, Klaus Zeyffert, Lukas Schleheck, David Leist, Marcel Polarz, Sebastian |
author_facet | Kunkel, Marius Schildknecht, Stefan Boldt, Klaus Zeyffert, Lukas Schleheck, David Leist, Marcel Polarz, Sebastian |
author_sort | Kunkel, Marius |
collection | PubMed |
description | [Image: see text] The importation of construction principles or even constituents from biology into materials science is a prevailing concept. Vice versa, the cellular level modification of living systems with nonnatural components is much more difficult to achieve. It has been done for analytical purposes, for example, imaging, to learn something about intracellular processes. Cases describing the improvement of a biological function by the integration of a nonnatural (nano)constituent are extremely rare. Because biological membranes contain some kind of a surfactant, for example, phospholipids, our idea is to modify cells with a newly synthesized surfactant. However, this surfactant is intended to possess an additional functionality, which is the reduction of oxidative stress. We report the synthesis of a surfactant with Janus-type head group architecture, a fullerene C(60) modified by five alkyl chains on one side and an average of 20 oxygen species on the other hemisphere. It is demonstrated that the amphiphilic properties of the fullerenol surfactant are similar to that of lipids. Not only quenching of reactive oxygen species (superoxide, hydroxyl radicals, peroxynitrite, and hydrogen peroxide) was successful, but also the fullerenol surfactant exceeds benchmark antioxidant agents such as quercetin. The surfactant was then brought into contact with different cell types, and the viability even of delicate cells such as human liver cells (HepG2) and human dopaminergic neurons (LUHMES) has proven to be extraordinarily high. We could show further that the cells take up the fullerenol surfactant, and as a consequence, they are protected much better against oxidative stress. |
format | Online Article Text |
id | pubmed-6091502 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | American
Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-60915022019-06-27 Increasing the Resistance of Living Cells against Oxidative Stress by Nonnatural Surfactants as Membrane Guards Kunkel, Marius Schildknecht, Stefan Boldt, Klaus Zeyffert, Lukas Schleheck, David Leist, Marcel Polarz, Sebastian ACS Appl Mater Interfaces [Image: see text] The importation of construction principles or even constituents from biology into materials science is a prevailing concept. Vice versa, the cellular level modification of living systems with nonnatural components is much more difficult to achieve. It has been done for analytical purposes, for example, imaging, to learn something about intracellular processes. Cases describing the improvement of a biological function by the integration of a nonnatural (nano)constituent are extremely rare. Because biological membranes contain some kind of a surfactant, for example, phospholipids, our idea is to modify cells with a newly synthesized surfactant. However, this surfactant is intended to possess an additional functionality, which is the reduction of oxidative stress. We report the synthesis of a surfactant with Janus-type head group architecture, a fullerene C(60) modified by five alkyl chains on one side and an average of 20 oxygen species on the other hemisphere. It is demonstrated that the amphiphilic properties of the fullerenol surfactant are similar to that of lipids. Not only quenching of reactive oxygen species (superoxide, hydroxyl radicals, peroxynitrite, and hydrogen peroxide) was successful, but also the fullerenol surfactant exceeds benchmark antioxidant agents such as quercetin. The surfactant was then brought into contact with different cell types, and the viability even of delicate cells such as human liver cells (HepG2) and human dopaminergic neurons (LUHMES) has proven to be extraordinarily high. We could show further that the cells take up the fullerenol surfactant, and as a consequence, they are protected much better against oxidative stress. American Chemical Society 2018-06-27 2018-07-18 /pmc/articles/PMC6091502/ /pubmed/29949339 http://dx.doi.org/10.1021/acsami.8b07032 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Kunkel, Marius Schildknecht, Stefan Boldt, Klaus Zeyffert, Lukas Schleheck, David Leist, Marcel Polarz, Sebastian Increasing the Resistance of Living Cells against Oxidative Stress by Nonnatural Surfactants as Membrane Guards |
title | Increasing
the Resistance of Living Cells against Oxidative Stress by Nonnatural
Surfactants as Membrane Guards |
title_full | Increasing
the Resistance of Living Cells against Oxidative Stress by Nonnatural
Surfactants as Membrane Guards |
title_fullStr | Increasing
the Resistance of Living Cells against Oxidative Stress by Nonnatural
Surfactants as Membrane Guards |
title_full_unstemmed | Increasing
the Resistance of Living Cells against Oxidative Stress by Nonnatural
Surfactants as Membrane Guards |
title_short | Increasing
the Resistance of Living Cells against Oxidative Stress by Nonnatural
Surfactants as Membrane Guards |
title_sort | increasing
the resistance of living cells against oxidative stress by nonnatural
surfactants as membrane guards |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6091502/ https://www.ncbi.nlm.nih.gov/pubmed/29949339 http://dx.doi.org/10.1021/acsami.8b07032 |
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