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Synovial Fluid Metabolites Differentiate between Septic and Nonseptic Joint Pathologies
[Image: see text] Osteoarthritis (OA), osteochondrosis (OC), and synovial sepsis in horses cause loss of function and pain. Reliable biomarkers are required to achieve accurate and rapid diagnosis, with synovial fluid (SF) holding a unique source of biochemical information. Nuclear magnetic resonanc...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6092013/ https://www.ncbi.nlm.nih.gov/pubmed/29969035 http://dx.doi.org/10.1021/acs.jproteome.8b00190 |
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author | Anderson, James R. Phelan, Marie M. Clegg, Peter D. Peffers, Mandy J. Rubio-Martinez, Luis M. |
author_facet | Anderson, James R. Phelan, Marie M. Clegg, Peter D. Peffers, Mandy J. Rubio-Martinez, Luis M. |
author_sort | Anderson, James R. |
collection | PubMed |
description | [Image: see text] Osteoarthritis (OA), osteochondrosis (OC), and synovial sepsis in horses cause loss of function and pain. Reliable biomarkers are required to achieve accurate and rapid diagnosis, with synovial fluid (SF) holding a unique source of biochemical information. Nuclear magnetic resonance (NMR) spectroscopy allows global metabolite analysis of a small volume of SF, with minimal sample preprocessing using a noninvasive and nondestructive method. Equine SF metabolic profiles from both nonseptic joints (OA and OC) and septic joints were analyzed using 1D (1)H NMR spectroscopy. Univariate and multivariate statistical analyses were used to identify differential metabolite abundance between groups. Metabolites were annotated via (1)H NMR using 1D NMR identification software Chenomx, with identities confirmed using 1D (1)H and 2D (1)H (13)C NMR. Multivariate analysis identified separation between septic and nonseptic groups. Acetate, alanine, citrate, creatine phosphate, creatinine, glucose, glutamate, glutamine, glycine, phenylalanine, pyruvate, and valine were higher in the nonseptic group, while glycylproline was higher in sepsis. Multivariate separation was primarily driven by glucose; however, partial-least-squares discriminant analysis plots with glucose excluded demonstrated the remaining metabolites were still able to discriminate the groups. This study demonstrates that a panel of synovial metabolites can distinguish between septic and nonseptic equine SF, with glucose the principal discriminator. |
format | Online Article Text |
id | pubmed-6092013 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-60920132018-08-15 Synovial Fluid Metabolites Differentiate between Septic and Nonseptic Joint Pathologies Anderson, James R. Phelan, Marie M. Clegg, Peter D. Peffers, Mandy J. Rubio-Martinez, Luis M. J Proteome Res [Image: see text] Osteoarthritis (OA), osteochondrosis (OC), and synovial sepsis in horses cause loss of function and pain. Reliable biomarkers are required to achieve accurate and rapid diagnosis, with synovial fluid (SF) holding a unique source of biochemical information. Nuclear magnetic resonance (NMR) spectroscopy allows global metabolite analysis of a small volume of SF, with minimal sample preprocessing using a noninvasive and nondestructive method. Equine SF metabolic profiles from both nonseptic joints (OA and OC) and septic joints were analyzed using 1D (1)H NMR spectroscopy. Univariate and multivariate statistical analyses were used to identify differential metabolite abundance between groups. Metabolites were annotated via (1)H NMR using 1D NMR identification software Chenomx, with identities confirmed using 1D (1)H and 2D (1)H (13)C NMR. Multivariate analysis identified separation between septic and nonseptic groups. Acetate, alanine, citrate, creatine phosphate, creatinine, glucose, glutamate, glutamine, glycine, phenylalanine, pyruvate, and valine were higher in the nonseptic group, while glycylproline was higher in sepsis. Multivariate separation was primarily driven by glucose; however, partial-least-squares discriminant analysis plots with glucose excluded demonstrated the remaining metabolites were still able to discriminate the groups. This study demonstrates that a panel of synovial metabolites can distinguish between septic and nonseptic equine SF, with glucose the principal discriminator. American Chemical Society 2018-07-03 2018-08-03 /pmc/articles/PMC6092013/ /pubmed/29969035 http://dx.doi.org/10.1021/acs.jproteome.8b00190 Text en Copyright © 2018 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
spellingShingle | Anderson, James R. Phelan, Marie M. Clegg, Peter D. Peffers, Mandy J. Rubio-Martinez, Luis M. Synovial Fluid Metabolites Differentiate between Septic and Nonseptic Joint Pathologies |
title | Synovial Fluid
Metabolites Differentiate between Septic
and Nonseptic Joint Pathologies |
title_full | Synovial Fluid
Metabolites Differentiate between Septic
and Nonseptic Joint Pathologies |
title_fullStr | Synovial Fluid
Metabolites Differentiate between Septic
and Nonseptic Joint Pathologies |
title_full_unstemmed | Synovial Fluid
Metabolites Differentiate between Septic
and Nonseptic Joint Pathologies |
title_short | Synovial Fluid
Metabolites Differentiate between Septic
and Nonseptic Joint Pathologies |
title_sort | synovial fluid
metabolites differentiate between septic
and nonseptic joint pathologies |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6092013/ https://www.ncbi.nlm.nih.gov/pubmed/29969035 http://dx.doi.org/10.1021/acs.jproteome.8b00190 |
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