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Oxidative stress decreases the redox ratio and folate content in the gut microbe, Enterococcus durans (MTCC 3031)
Gut microbiome plays an important role in determining the effectiveness of cancer therapy. The composition of the microbiome is crucial to maintain good digestive health in the host, and to prevent and treat colorectal cancers. Most cancer therapies employ oxidative stress, which disturbs the redox...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6092354/ https://www.ncbi.nlm.nih.gov/pubmed/30108274 http://dx.doi.org/10.1038/s41598-018-30691-4 |
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author | Jose, Steffi Bhalla, Prerna Suraishkumar, G. K. |
author_facet | Jose, Steffi Bhalla, Prerna Suraishkumar, G. K. |
author_sort | Jose, Steffi |
collection | PubMed |
description | Gut microbiome plays an important role in determining the effectiveness of cancer therapy. The composition of the microbiome is crucial to maintain good digestive health in the host, and to prevent and treat colorectal cancers. Most cancer therapies employ oxidative stress, which disturbs the redox status of the cell, and consequently affect growth, reductive biosynthesis and cell death. Therefore, oxidative stress can undesirably affect the gut microbiome. Hence, it is important to understand the impact of oxidative stress on gut bacteria to devise effective treatment strategies. The current study induces oxidative stress in the model gut bacterium Enterococcus durans (MTCC 3031) with menadione and H(2)O(2). Oxidative stress considerably decreased the redox ratio (NADPH/NADP), an indicator of the redox status, by 55% (menadione) and 28% (H(2)O(2)). In addition, an oxidative stress induced decrease in redox ratio decreased folate synthesis by the bacteria, which is an undesirable consequence for the host, since folate deficiency can induce colorectal cancer. Further, oxidative stress considerably decreased growth and the biomass density by 61% (menadione) and 21% (H(2)O(2)). Thus, maintenance of the cellular redox status and management of oxidative stress in the gut microbiome may be crucial to the effectiveness of cancer treatment strategies. |
format | Online Article Text |
id | pubmed-6092354 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-60923542018-08-20 Oxidative stress decreases the redox ratio and folate content in the gut microbe, Enterococcus durans (MTCC 3031) Jose, Steffi Bhalla, Prerna Suraishkumar, G. K. Sci Rep Article Gut microbiome plays an important role in determining the effectiveness of cancer therapy. The composition of the microbiome is crucial to maintain good digestive health in the host, and to prevent and treat colorectal cancers. Most cancer therapies employ oxidative stress, which disturbs the redox status of the cell, and consequently affect growth, reductive biosynthesis and cell death. Therefore, oxidative stress can undesirably affect the gut microbiome. Hence, it is important to understand the impact of oxidative stress on gut bacteria to devise effective treatment strategies. The current study induces oxidative stress in the model gut bacterium Enterococcus durans (MTCC 3031) with menadione and H(2)O(2). Oxidative stress considerably decreased the redox ratio (NADPH/NADP), an indicator of the redox status, by 55% (menadione) and 28% (H(2)O(2)). In addition, an oxidative stress induced decrease in redox ratio decreased folate synthesis by the bacteria, which is an undesirable consequence for the host, since folate deficiency can induce colorectal cancer. Further, oxidative stress considerably decreased growth and the biomass density by 61% (menadione) and 21% (H(2)O(2)). Thus, maintenance of the cellular redox status and management of oxidative stress in the gut microbiome may be crucial to the effectiveness of cancer treatment strategies. Nature Publishing Group UK 2018-08-14 /pmc/articles/PMC6092354/ /pubmed/30108274 http://dx.doi.org/10.1038/s41598-018-30691-4 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Jose, Steffi Bhalla, Prerna Suraishkumar, G. K. Oxidative stress decreases the redox ratio and folate content in the gut microbe, Enterococcus durans (MTCC 3031) |
title | Oxidative stress decreases the redox ratio and folate content in the gut microbe, Enterococcus durans (MTCC 3031) |
title_full | Oxidative stress decreases the redox ratio and folate content in the gut microbe, Enterococcus durans (MTCC 3031) |
title_fullStr | Oxidative stress decreases the redox ratio and folate content in the gut microbe, Enterococcus durans (MTCC 3031) |
title_full_unstemmed | Oxidative stress decreases the redox ratio and folate content in the gut microbe, Enterococcus durans (MTCC 3031) |
title_short | Oxidative stress decreases the redox ratio and folate content in the gut microbe, Enterococcus durans (MTCC 3031) |
title_sort | oxidative stress decreases the redox ratio and folate content in the gut microbe, enterococcus durans (mtcc 3031) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6092354/ https://www.ncbi.nlm.nih.gov/pubmed/30108274 http://dx.doi.org/10.1038/s41598-018-30691-4 |
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