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The establishment of a marine focused biorefinery for bioethanol production using seawater and a novel marine yeast strain
Current technologies for bioethanol production rely on the use of freshwater for preparing the fermentation media and use yeasts of a terrestrial origin. Life cycle assessment has suggested that between 1,388 to 9,812 litres of freshwater are consumed for every litre of bioethanol produced. Hence, b...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6092365/ https://www.ncbi.nlm.nih.gov/pubmed/30108287 http://dx.doi.org/10.1038/s41598-018-30660-x |
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author | Zaky, Abdelrahman Saleh Greetham, Darren Tucker, Gregory A. Du, Chenyu |
author_facet | Zaky, Abdelrahman Saleh Greetham, Darren Tucker, Gregory A. Du, Chenyu |
author_sort | Zaky, Abdelrahman Saleh |
collection | PubMed |
description | Current technologies for bioethanol production rely on the use of freshwater for preparing the fermentation media and use yeasts of a terrestrial origin. Life cycle assessment has suggested that between 1,388 to 9,812 litres of freshwater are consumed for every litre of bioethanol produced. Hence, bioethanol is considered a product with a high-water footprint. This paper investigated the use of seawater-based media and a novel marine yeast strain ‘Saccharomyces cerevisiae AZ65’ to reduce the water footprint of bioethanol. Results revealed that S. cerevisiae AZ65 had a significantly higher osmotic tolerance when compared with the terrestrial reference strain. Using 15-L bioreactors, S. cerevisiae AZ65 produced 93.50 g/L ethanol with a yield of 83.33% (of the theoretical yield) and a maximum productivity of 2.49 g/L/h when using seawater-YPD media. This approach was successfully applied using an industrial fermentation substrate (sugarcane molasses). S. cerevisiae AZ65 produced 52.23 g/L ethanol using molasses media prepared in seawater with a yield of 73.80% (of the theoretical yield) and a maximum productivity of 1.43 g/L/h. These results demonstrated that seawater can substitute freshwater for bioethanol production without compromising production efficiency. Results also revealed that marine yeast is a potential candidate for use in the bioethanol industry especially when using seawater or high salt based fermentation media. |
format | Online Article Text |
id | pubmed-6092365 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-60923652018-08-20 The establishment of a marine focused biorefinery for bioethanol production using seawater and a novel marine yeast strain Zaky, Abdelrahman Saleh Greetham, Darren Tucker, Gregory A. Du, Chenyu Sci Rep Article Current technologies for bioethanol production rely on the use of freshwater for preparing the fermentation media and use yeasts of a terrestrial origin. Life cycle assessment has suggested that between 1,388 to 9,812 litres of freshwater are consumed for every litre of bioethanol produced. Hence, bioethanol is considered a product with a high-water footprint. This paper investigated the use of seawater-based media and a novel marine yeast strain ‘Saccharomyces cerevisiae AZ65’ to reduce the water footprint of bioethanol. Results revealed that S. cerevisiae AZ65 had a significantly higher osmotic tolerance when compared with the terrestrial reference strain. Using 15-L bioreactors, S. cerevisiae AZ65 produced 93.50 g/L ethanol with a yield of 83.33% (of the theoretical yield) and a maximum productivity of 2.49 g/L/h when using seawater-YPD media. This approach was successfully applied using an industrial fermentation substrate (sugarcane molasses). S. cerevisiae AZ65 produced 52.23 g/L ethanol using molasses media prepared in seawater with a yield of 73.80% (of the theoretical yield) and a maximum productivity of 1.43 g/L/h. These results demonstrated that seawater can substitute freshwater for bioethanol production without compromising production efficiency. Results also revealed that marine yeast is a potential candidate for use in the bioethanol industry especially when using seawater or high salt based fermentation media. Nature Publishing Group UK 2018-08-14 /pmc/articles/PMC6092365/ /pubmed/30108287 http://dx.doi.org/10.1038/s41598-018-30660-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Zaky, Abdelrahman Saleh Greetham, Darren Tucker, Gregory A. Du, Chenyu The establishment of a marine focused biorefinery for bioethanol production using seawater and a novel marine yeast strain |
title | The establishment of a marine focused biorefinery for bioethanol production using seawater and a novel marine yeast strain |
title_full | The establishment of a marine focused biorefinery for bioethanol production using seawater and a novel marine yeast strain |
title_fullStr | The establishment of a marine focused biorefinery for bioethanol production using seawater and a novel marine yeast strain |
title_full_unstemmed | The establishment of a marine focused biorefinery for bioethanol production using seawater and a novel marine yeast strain |
title_short | The establishment of a marine focused biorefinery for bioethanol production using seawater and a novel marine yeast strain |
title_sort | establishment of a marine focused biorefinery for bioethanol production using seawater and a novel marine yeast strain |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6092365/ https://www.ncbi.nlm.nih.gov/pubmed/30108287 http://dx.doi.org/10.1038/s41598-018-30660-x |
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