Specificity of RNAi, LNA and CRISPRi as loss-of-function methods in transcriptional analysis

Loss-of-function (LOF) methods such as RNA interference (RNAi), antisense oligonucleotides or CRISPR-based genome editing provide unparalleled power for studying the biological function of genes of interest. However, a major concern is non-specific targeting, which involves depletion of transcripts...

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Detalles Bibliográficos
Autores principales: Stojic, Lovorka, Lun, Aaron T L, Mangei, Jasmin, Mascalchi, Patrice, Quarantotti, Valentina, Barr, Alexis R, Bakal, Chris, Marioni, John C, Gergely, Fanni, Odom, Duncan T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Computational Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6093183/
https://www.ncbi.nlm.nih.gov/pubmed/29860520
http://dx.doi.org/10.1093/nar/gky437

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