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Proteomic analysis of the defense response to Magnaporthe oryzae in rice harboring the blast resistance gene Piz-t

BACKGROUND: Rice blast (caused by Magnaporthe oryzae) is one of the most destructive diseases of rice. While many blast resistance (R) genes have been identified and deployed in rice cultivars, little is known about the R gene-mediated defense mechanism. We used a rice transgenic line harboring the...

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Autores principales: Tian, Dagang, Yang, Liu, Chen, Zaijie, Chen, Ziqiang, Wang, Feng, Zhou, Yuanchang, Luo, Yuming, Yang, Liming, Chen, Songbiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6093832/
https://www.ncbi.nlm.nih.gov/pubmed/30112588
http://dx.doi.org/10.1186/s12284-018-0240-3
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author Tian, Dagang
Yang, Liu
Chen, Zaijie
Chen, Ziqiang
Wang, Feng
Zhou, Yuanchang
Luo, Yuming
Yang, Liming
Chen, Songbiao
author_facet Tian, Dagang
Yang, Liu
Chen, Zaijie
Chen, Ziqiang
Wang, Feng
Zhou, Yuanchang
Luo, Yuming
Yang, Liming
Chen, Songbiao
author_sort Tian, Dagang
collection PubMed
description BACKGROUND: Rice blast (caused by Magnaporthe oryzae) is one of the most destructive diseases of rice. While many blast resistance (R) genes have been identified and deployed in rice cultivars, little is known about the R gene-mediated defense mechanism. We used a rice transgenic line harboring the resistance gene Piz-t to investigate the R gene-mediated resistance response to infection. RESULTS: We conducted comparative proteome profiling of the Piz-t transgenic Nipponbare line (NPB-Piz-t) and wild-type Nipponbare (NPB) inoculated with M. oryzae at 24, 48, 72 h post-inoculation (hpi) using isobaric tags for relative and absolute quantification (iTRAQ) analysis. Comparative analysis of the response of NPB-Piz-t to the avirulent isolate KJ201 and the virulent isolate RB22 identified 114 differentially expressed proteins (DEPs) between KJ201-inoculated NPB-Piz-t (KJ201-Piz-t) and mock-treated NPB-Piz-t (Mock-Piz-t), and 118 DEPs between RB22-inoculated NPB-Piz-t (RB22-Piz-t) and Mock-Piz-t. Among the DEPs, 56 occurred commonly in comparisons KJ201-Piz-t/Mock-Piz-t and RB22-Piz-t/Mock-Piz-t. In a comparison of the responses of NPB and NPB-Piz-t to isolate KJ201, 93 DEPs between KJ201-Piz-t and KJ201-NPB were identified. DEPs in comparisons KJ201-Piz-t/Mock-Piz-t, RB22-Piz-t/Mock-Piz-t and KJ201-Piz-t/KJ201-NPB contained a number of proteins that may be involved in rice response to pathogens, including pathogenesis-related (PR) proteins, hormonal regulation-related proteins, defense and stress response-related proteins, receptor-like kinase, and cytochrome P450. Comparative analysis further identified 7 common DEPs between the comparisons KJ201-Piz-t/KJ201-NPB and KJ201-Piz-t/RB22-Piz-t, including alcohol dehydrogenase I, receptor-like protein kinase, endochitinase, similar to rubisco large subunit, NADP-dependent malic enzyme, and two hypothetical proteins. CONCLUSIONS: Our results provide a valuable resource for discovery of complex protein networks involved in the resistance response of rice to blast fungus. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12284-018-0240-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-60938322018-09-11 Proteomic analysis of the defense response to Magnaporthe oryzae in rice harboring the blast resistance gene Piz-t Tian, Dagang Yang, Liu Chen, Zaijie Chen, Ziqiang Wang, Feng Zhou, Yuanchang Luo, Yuming Yang, Liming Chen, Songbiao Rice (N Y) Original Article BACKGROUND: Rice blast (caused by Magnaporthe oryzae) is one of the most destructive diseases of rice. While many blast resistance (R) genes have been identified and deployed in rice cultivars, little is known about the R gene-mediated defense mechanism. We used a rice transgenic line harboring the resistance gene Piz-t to investigate the R gene-mediated resistance response to infection. RESULTS: We conducted comparative proteome profiling of the Piz-t transgenic Nipponbare line (NPB-Piz-t) and wild-type Nipponbare (NPB) inoculated with M. oryzae at 24, 48, 72 h post-inoculation (hpi) using isobaric tags for relative and absolute quantification (iTRAQ) analysis. Comparative analysis of the response of NPB-Piz-t to the avirulent isolate KJ201 and the virulent isolate RB22 identified 114 differentially expressed proteins (DEPs) between KJ201-inoculated NPB-Piz-t (KJ201-Piz-t) and mock-treated NPB-Piz-t (Mock-Piz-t), and 118 DEPs between RB22-inoculated NPB-Piz-t (RB22-Piz-t) and Mock-Piz-t. Among the DEPs, 56 occurred commonly in comparisons KJ201-Piz-t/Mock-Piz-t and RB22-Piz-t/Mock-Piz-t. In a comparison of the responses of NPB and NPB-Piz-t to isolate KJ201, 93 DEPs between KJ201-Piz-t and KJ201-NPB were identified. DEPs in comparisons KJ201-Piz-t/Mock-Piz-t, RB22-Piz-t/Mock-Piz-t and KJ201-Piz-t/KJ201-NPB contained a number of proteins that may be involved in rice response to pathogens, including pathogenesis-related (PR) proteins, hormonal regulation-related proteins, defense and stress response-related proteins, receptor-like kinase, and cytochrome P450. Comparative analysis further identified 7 common DEPs between the comparisons KJ201-Piz-t/KJ201-NPB and KJ201-Piz-t/RB22-Piz-t, including alcohol dehydrogenase I, receptor-like protein kinase, endochitinase, similar to rubisco large subunit, NADP-dependent malic enzyme, and two hypothetical proteins. CONCLUSIONS: Our results provide a valuable resource for discovery of complex protein networks involved in the resistance response of rice to blast fungus. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12284-018-0240-3) contains supplementary material, which is available to authorized users. Springer US 2018-08-15 /pmc/articles/PMC6093832/ /pubmed/30112588 http://dx.doi.org/10.1186/s12284-018-0240-3 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Tian, Dagang
Yang, Liu
Chen, Zaijie
Chen, Ziqiang
Wang, Feng
Zhou, Yuanchang
Luo, Yuming
Yang, Liming
Chen, Songbiao
Proteomic analysis of the defense response to Magnaporthe oryzae in rice harboring the blast resistance gene Piz-t
title Proteomic analysis of the defense response to Magnaporthe oryzae in rice harboring the blast resistance gene Piz-t
title_full Proteomic analysis of the defense response to Magnaporthe oryzae in rice harboring the blast resistance gene Piz-t
title_fullStr Proteomic analysis of the defense response to Magnaporthe oryzae in rice harboring the blast resistance gene Piz-t
title_full_unstemmed Proteomic analysis of the defense response to Magnaporthe oryzae in rice harboring the blast resistance gene Piz-t
title_short Proteomic analysis of the defense response to Magnaporthe oryzae in rice harboring the blast resistance gene Piz-t
title_sort proteomic analysis of the defense response to magnaporthe oryzae in rice harboring the blast resistance gene piz-t
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6093832/
https://www.ncbi.nlm.nih.gov/pubmed/30112588
http://dx.doi.org/10.1186/s12284-018-0240-3
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