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Wound Healing in Streptozotocin-Induced Diabetic Rats Using Atmospheric-Pressure Argon Plasma Jet

In this study, we used an argon-based round atmospheric-pressure plasma jet (APPJ) for enhancing wound healing in streptozotocin (STZ) induced diabetic rats. The APPJ was characterized by optical emission spectroscopy. We induced Type 1 and Type 2 diabetes in rats with different amounts of STZ combi...

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Autores principales: Cheng, Kuang-Yao, Lin, Zhi-Hua, Cheng, Yu-Pin, Chiu, Hsien-Yi, Yeh, Nai-Lun, Wu, Tung-Kung, Wu, Jong-Shinn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6093903/
https://www.ncbi.nlm.nih.gov/pubmed/30111887
http://dx.doi.org/10.1038/s41598-018-30597-1
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author Cheng, Kuang-Yao
Lin, Zhi-Hua
Cheng, Yu-Pin
Chiu, Hsien-Yi
Yeh, Nai-Lun
Wu, Tung-Kung
Wu, Jong-Shinn
author_facet Cheng, Kuang-Yao
Lin, Zhi-Hua
Cheng, Yu-Pin
Chiu, Hsien-Yi
Yeh, Nai-Lun
Wu, Tung-Kung
Wu, Jong-Shinn
author_sort Cheng, Kuang-Yao
collection PubMed
description In this study, we used an argon-based round atmospheric-pressure plasma jet (APPJ) for enhancing wound healing in streptozotocin (STZ) induced diabetic rats. The APPJ was characterized by optical emission spectroscopy. We induced Type 1 and Type 2 diabetes in rats with different amounts of STZ combined with normal and high-fat diets, respectively. The wound area ratio of all the plasma-treated normal and diabetic groups was greatly reduced (up to 30%) compared with that of the untreated groups during healing. Histological analysis revealed faster re-epithelialization, collagen deposition, less inflammation, and a complete skin structure in the plasma-treated groups was found as compared with the untreated control groups. In addition, the new blood vessels of plasma-treated tissues decreased more than untreated tissues in the middle (Day 14) and late (Day 21) stages of wound healing. The plasma-treated wounds demonstrated more transforming growth factor beta (TGF-β) expression in the early stage (Day 7), whereas they decreased in the middle and late stages of wound healing. The levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) increased after plasma treatment. In addition, plasma-treated water had a higher concentration of hydrogen peroxide, nitrite and nitrate when the plasma treatment time was longer. In summary, the proposed argon APPJ based on the current study could be a potential tool for treating diabetic wounds.
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spelling pubmed-60939032018-08-20 Wound Healing in Streptozotocin-Induced Diabetic Rats Using Atmospheric-Pressure Argon Plasma Jet Cheng, Kuang-Yao Lin, Zhi-Hua Cheng, Yu-Pin Chiu, Hsien-Yi Yeh, Nai-Lun Wu, Tung-Kung Wu, Jong-Shinn Sci Rep Article In this study, we used an argon-based round atmospheric-pressure plasma jet (APPJ) for enhancing wound healing in streptozotocin (STZ) induced diabetic rats. The APPJ was characterized by optical emission spectroscopy. We induced Type 1 and Type 2 diabetes in rats with different amounts of STZ combined with normal and high-fat diets, respectively. The wound area ratio of all the plasma-treated normal and diabetic groups was greatly reduced (up to 30%) compared with that of the untreated groups during healing. Histological analysis revealed faster re-epithelialization, collagen deposition, less inflammation, and a complete skin structure in the plasma-treated groups was found as compared with the untreated control groups. In addition, the new blood vessels of plasma-treated tissues decreased more than untreated tissues in the middle (Day 14) and late (Day 21) stages of wound healing. The plasma-treated wounds demonstrated more transforming growth factor beta (TGF-β) expression in the early stage (Day 7), whereas they decreased in the middle and late stages of wound healing. The levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) increased after plasma treatment. In addition, plasma-treated water had a higher concentration of hydrogen peroxide, nitrite and nitrate when the plasma treatment time was longer. In summary, the proposed argon APPJ based on the current study could be a potential tool for treating diabetic wounds. Nature Publishing Group UK 2018-08-15 /pmc/articles/PMC6093903/ /pubmed/30111887 http://dx.doi.org/10.1038/s41598-018-30597-1 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Cheng, Kuang-Yao
Lin, Zhi-Hua
Cheng, Yu-Pin
Chiu, Hsien-Yi
Yeh, Nai-Lun
Wu, Tung-Kung
Wu, Jong-Shinn
Wound Healing in Streptozotocin-Induced Diabetic Rats Using Atmospheric-Pressure Argon Plasma Jet
title Wound Healing in Streptozotocin-Induced Diabetic Rats Using Atmospheric-Pressure Argon Plasma Jet
title_full Wound Healing in Streptozotocin-Induced Diabetic Rats Using Atmospheric-Pressure Argon Plasma Jet
title_fullStr Wound Healing in Streptozotocin-Induced Diabetic Rats Using Atmospheric-Pressure Argon Plasma Jet
title_full_unstemmed Wound Healing in Streptozotocin-Induced Diabetic Rats Using Atmospheric-Pressure Argon Plasma Jet
title_short Wound Healing in Streptozotocin-Induced Diabetic Rats Using Atmospheric-Pressure Argon Plasma Jet
title_sort wound healing in streptozotocin-induced diabetic rats using atmospheric-pressure argon plasma jet
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6093903/
https://www.ncbi.nlm.nih.gov/pubmed/30111887
http://dx.doi.org/10.1038/s41598-018-30597-1
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