Cargando…

Unbiased classification of mosquito blood cells by single-cell genomics and high-content imaging

Mosquito blood cells are immune cells that help control infection by vector-borne pathogens. Despite their importance, little is known about mosquito blood cell biology beyond morphological and functional criteria used for their classification. Here, we combined the power of single-cell RNA sequenci...

Descripción completa

Detalles Bibliográficos
Autores principales: Severo, Maiara S., Landry, Jonathan J. M., Lindquist, Randall L., Goosmann, Christian, Brinkmann, Volker, Collier, Paul, Hauser, Anja E., Benes, Vladimir, Henriksson, Johan, Teichmann, Sarah A., Levashina, Elena A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6094101/
https://www.ncbi.nlm.nih.gov/pubmed/30038005
http://dx.doi.org/10.1073/pnas.1803062115
Descripción
Sumario:Mosquito blood cells are immune cells that help control infection by vector-borne pathogens. Despite their importance, little is known about mosquito blood cell biology beyond morphological and functional criteria used for their classification. Here, we combined the power of single-cell RNA sequencing, high-content imaging flow cytometry, and single-molecule RNA hybridization to analyze a subset of blood cells of the malaria mosquito Anopheles gambiae. By demonstrating that blood cells express nearly half of the mosquito transcriptome, our dataset represents an unprecedented view into their transcriptional program. Analyses of differentially expressed genes identified transcriptional signatures of two cell types and provide insights into the current classification of these cells. We further demonstrate the active transfer of a cellular marker between blood cells that may confound their identification. We propose that cell-to-cell exchange may contribute to cellular diversity and functional plasticity seen across biological systems.