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Generation of renewable mouse intestinal epithelial cell monolayers and organoids for functional analyses

BACKGROUND: Conditional reprogramming has enabled the development of long-lived, normal epithelial cell lines from mice and humans by in vitro culture with ROCK inhibitor on a feeder layer. We applied this technology to mouse small intestine to create 2D mouse intestinal epithelial monolayers (IEC m...

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Detalles Bibliográficos
Autores principales:	Moorefield, Emily C., Blue, R. Eric, Quinney, Nancy L., Gentzsch, Martina, Ding, Shengli
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2018
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6094565/ https://www.ncbi.nlm.nih.gov/pubmed/30111276 http://dx.doi.org/10.1186/s12860-018-0165-0

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