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Allergic Asthma Favors Brucella Growth in the Lungs of Infected Mice

Allergic asthma is a chronic Th2 inflammatory disease of the lower airways affecting a growing number of people worldwide. The impact of infections and microbiota composition on allergic asthma has been investigated frequently. Until now, however, there have been few attempts to investigate the impa...

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Autores principales: Machelart, Arnaud, Potemberg, Georges, Van Maele, Laurye, Demars, Aurore, Lagneaux, Maxime, De Trez, Carl, Sabatel, Catherine, Bureau, Fabrice, De Prins, Sofie, Percier, Pauline, Denis, Olivier, Jurion, Fabienne, Romano, Marta, Vanderwinden, Jean-Marie, Letesson, Jean-Jacques, Muraille, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6095999/
https://www.ncbi.nlm.nih.gov/pubmed/30147700
http://dx.doi.org/10.3389/fimmu.2018.01856
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author Machelart, Arnaud
Potemberg, Georges
Van Maele, Laurye
Demars, Aurore
Lagneaux, Maxime
De Trez, Carl
Sabatel, Catherine
Bureau, Fabrice
De Prins, Sofie
Percier, Pauline
Denis, Olivier
Jurion, Fabienne
Romano, Marta
Vanderwinden, Jean-Marie
Letesson, Jean-Jacques
Muraille, Eric
author_facet Machelart, Arnaud
Potemberg, Georges
Van Maele, Laurye
Demars, Aurore
Lagneaux, Maxime
De Trez, Carl
Sabatel, Catherine
Bureau, Fabrice
De Prins, Sofie
Percier, Pauline
Denis, Olivier
Jurion, Fabienne
Romano, Marta
Vanderwinden, Jean-Marie
Letesson, Jean-Jacques
Muraille, Eric
author_sort Machelart, Arnaud
collection PubMed
description Allergic asthma is a chronic Th2 inflammatory disease of the lower airways affecting a growing number of people worldwide. The impact of infections and microbiota composition on allergic asthma has been investigated frequently. Until now, however, there have been few attempts to investigate the impact of asthma on the control of infectious microorganisms and the underlying mechanisms. In this work, we characterize the consequences of allergic asthma on intranasal (i.n.) infection by Brucella bacteria in mice. We observed that i.n. sensitization with extracts of the house dust mite Dermatophagoides farinae or the mold Alternaria alternata (Alt) significantly increased the number of Brucella melitensis, Brucella suis, and Brucella abortus in the lungs of infected mice. Microscopic analysis showed dense aggregates of infected cells composed mainly of alveolar macrophages (CD11c(+) F4/80(+) MHCII(+)) surrounded by neutrophils (Ly-6G(+)). Asthma-induced Brucella susceptibility appears to be dependent on CD4(+) T cells, the IL-4/STAT6 signaling pathway and IL-10, and is maintained in IL-12- and IFN-γR-deficient mice. The effects of the Alt sensitization protocol were also tested on Streptococcus pneumoniae and Mycobacterium tuberculosis pulmonary infections. Surprisingly, we observed that Alt sensitization strongly increases the survival of S. pneumoniae infected mice by a T cell and STAT6 independent signaling pathway. In contrast, the course of M. tuberculosis infection is not affected in the lungs of sensitized mice. Our work demonstrates that the impact of the same allergic sensitization protocol can be neutral, negative, or positive with regard to the resistance of mice to bacterial infection, depending on the bacterial species.
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spelling pubmed-60959992018-08-24 Allergic Asthma Favors Brucella Growth in the Lungs of Infected Mice Machelart, Arnaud Potemberg, Georges Van Maele, Laurye Demars, Aurore Lagneaux, Maxime De Trez, Carl Sabatel, Catherine Bureau, Fabrice De Prins, Sofie Percier, Pauline Denis, Olivier Jurion, Fabienne Romano, Marta Vanderwinden, Jean-Marie Letesson, Jean-Jacques Muraille, Eric Front Immunol Immunology Allergic asthma is a chronic Th2 inflammatory disease of the lower airways affecting a growing number of people worldwide. The impact of infections and microbiota composition on allergic asthma has been investigated frequently. Until now, however, there have been few attempts to investigate the impact of asthma on the control of infectious microorganisms and the underlying mechanisms. In this work, we characterize the consequences of allergic asthma on intranasal (i.n.) infection by Brucella bacteria in mice. We observed that i.n. sensitization with extracts of the house dust mite Dermatophagoides farinae or the mold Alternaria alternata (Alt) significantly increased the number of Brucella melitensis, Brucella suis, and Brucella abortus in the lungs of infected mice. Microscopic analysis showed dense aggregates of infected cells composed mainly of alveolar macrophages (CD11c(+) F4/80(+) MHCII(+)) surrounded by neutrophils (Ly-6G(+)). Asthma-induced Brucella susceptibility appears to be dependent on CD4(+) T cells, the IL-4/STAT6 signaling pathway and IL-10, and is maintained in IL-12- and IFN-γR-deficient mice. The effects of the Alt sensitization protocol were also tested on Streptococcus pneumoniae and Mycobacterium tuberculosis pulmonary infections. Surprisingly, we observed that Alt sensitization strongly increases the survival of S. pneumoniae infected mice by a T cell and STAT6 independent signaling pathway. In contrast, the course of M. tuberculosis infection is not affected in the lungs of sensitized mice. Our work demonstrates that the impact of the same allergic sensitization protocol can be neutral, negative, or positive with regard to the resistance of mice to bacterial infection, depending on the bacterial species. Frontiers Media S.A. 2018-08-10 /pmc/articles/PMC6095999/ /pubmed/30147700 http://dx.doi.org/10.3389/fimmu.2018.01856 Text en Copyright © 2018 Machelart, Potemberg, Van Maele, Demars, Lagneaux, De Trez, Sabatel, Bureau, De Prins, Percier, Denis, Jurion, Romano, Vanderwinden, Letesson and Muraille. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Machelart, Arnaud
Potemberg, Georges
Van Maele, Laurye
Demars, Aurore
Lagneaux, Maxime
De Trez, Carl
Sabatel, Catherine
Bureau, Fabrice
De Prins, Sofie
Percier, Pauline
Denis, Olivier
Jurion, Fabienne
Romano, Marta
Vanderwinden, Jean-Marie
Letesson, Jean-Jacques
Muraille, Eric
Allergic Asthma Favors Brucella Growth in the Lungs of Infected Mice
title Allergic Asthma Favors Brucella Growth in the Lungs of Infected Mice
title_full Allergic Asthma Favors Brucella Growth in the Lungs of Infected Mice
title_fullStr Allergic Asthma Favors Brucella Growth in the Lungs of Infected Mice
title_full_unstemmed Allergic Asthma Favors Brucella Growth in the Lungs of Infected Mice
title_short Allergic Asthma Favors Brucella Growth in the Lungs of Infected Mice
title_sort allergic asthma favors brucella growth in the lungs of infected mice
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6095999/
https://www.ncbi.nlm.nih.gov/pubmed/30147700
http://dx.doi.org/10.3389/fimmu.2018.01856
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