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Homoisoflavanone-1 isolated from Polygonatum odoratum arrests the cell cycle and induces apoptosis in A549 cells

Homoisoflavanone-1 is a natural compound that may be extracted from the Chinese medicinal herb Polygonatum odoratum, which has pronounced antioxidant activities. The present study reports that homoisoflavanone-1 significantly inhibited tumor cell growth and induced apoptosis in A549 non-small cell l...

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Autores principales: Ning, Deli, Jin, Ming, Xv, Tao, Sun, Jikai, Li, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6096101/
https://www.ncbi.nlm.nih.gov/pubmed/30127960
http://dx.doi.org/10.3892/ol.2018.9085
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author Ning, Deli
Jin, Ming
Xv, Tao
Sun, Jikai
Li, Min
author_facet Ning, Deli
Jin, Ming
Xv, Tao
Sun, Jikai
Li, Min
author_sort Ning, Deli
collection PubMed
description Homoisoflavanone-1 is a natural compound that may be extracted from the Chinese medicinal herb Polygonatum odoratum, which has pronounced antioxidant activities. The present study reports that homoisoflavanone-1 significantly inhibited tumor cell growth and induced apoptosis in A549 non-small cell lung cancer (NSCLC) cells in a dose-dependent manner. Homoisoflavanone-1 arrested the cell cycle at the G2/M stage, which was associated with an increase in the accumulation of phosphorylated (p-)p38, p38, p53, and p-cyclin dependent kinase (Cdc)2 proteins, as well as a decrease in Cdc2 expression. In addition, treatment with homoisoflavanone-1 increased the levels of active caspase-3 and decreased Poly ADP-ribose polymerase, which was accompanied by a reduction in the B-cell lymphoma-2/Bak ratio and consequently, apoptosis. Furthermore, homoisoflavanone-1 increased the expression of endoplasmic reticulum (ER) stress-related proteins, including PERK, ATF4 and GADD34 in a dose-dependent manner. In conclusion, homoisoflavanone-1 induced apoptosis in A549 cells by regulating the mitochondria-caspase-dependent and ER stress pathways and resulted in G2/M arrest by activating the p38/p53 signaling pathway. These findings suggest that homoisoflavanone-1 extracted from Polygonatum odoratum may function as a cancer-suppressing agent and has potential as a novel therapeutic method against NSCLC.
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spelling pubmed-60961012018-08-20 Homoisoflavanone-1 isolated from Polygonatum odoratum arrests the cell cycle and induces apoptosis in A549 cells Ning, Deli Jin, Ming Xv, Tao Sun, Jikai Li, Min Oncol Lett Articles Homoisoflavanone-1 is a natural compound that may be extracted from the Chinese medicinal herb Polygonatum odoratum, which has pronounced antioxidant activities. The present study reports that homoisoflavanone-1 significantly inhibited tumor cell growth and induced apoptosis in A549 non-small cell lung cancer (NSCLC) cells in a dose-dependent manner. Homoisoflavanone-1 arrested the cell cycle at the G2/M stage, which was associated with an increase in the accumulation of phosphorylated (p-)p38, p38, p53, and p-cyclin dependent kinase (Cdc)2 proteins, as well as a decrease in Cdc2 expression. In addition, treatment with homoisoflavanone-1 increased the levels of active caspase-3 and decreased Poly ADP-ribose polymerase, which was accompanied by a reduction in the B-cell lymphoma-2/Bak ratio and consequently, apoptosis. Furthermore, homoisoflavanone-1 increased the expression of endoplasmic reticulum (ER) stress-related proteins, including PERK, ATF4 and GADD34 in a dose-dependent manner. In conclusion, homoisoflavanone-1 induced apoptosis in A549 cells by regulating the mitochondria-caspase-dependent and ER stress pathways and resulted in G2/M arrest by activating the p38/p53 signaling pathway. These findings suggest that homoisoflavanone-1 extracted from Polygonatum odoratum may function as a cancer-suppressing agent and has potential as a novel therapeutic method against NSCLC. D.A. Spandidos 2018-09 2018-07-05 /pmc/articles/PMC6096101/ /pubmed/30127960 http://dx.doi.org/10.3892/ol.2018.9085 Text en Copyright: © Ning et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Ning, Deli
Jin, Ming
Xv, Tao
Sun, Jikai
Li, Min
Homoisoflavanone-1 isolated from Polygonatum odoratum arrests the cell cycle and induces apoptosis in A549 cells
title Homoisoflavanone-1 isolated from Polygonatum odoratum arrests the cell cycle and induces apoptosis in A549 cells
title_full Homoisoflavanone-1 isolated from Polygonatum odoratum arrests the cell cycle and induces apoptosis in A549 cells
title_fullStr Homoisoflavanone-1 isolated from Polygonatum odoratum arrests the cell cycle and induces apoptosis in A549 cells
title_full_unstemmed Homoisoflavanone-1 isolated from Polygonatum odoratum arrests the cell cycle and induces apoptosis in A549 cells
title_short Homoisoflavanone-1 isolated from Polygonatum odoratum arrests the cell cycle and induces apoptosis in A549 cells
title_sort homoisoflavanone-1 isolated from polygonatum odoratum arrests the cell cycle and induces apoptosis in a549 cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6096101/
https://www.ncbi.nlm.nih.gov/pubmed/30127960
http://dx.doi.org/10.3892/ol.2018.9085
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