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miR-18a-5p promotes cell invasion and migration of osteosarcoma by directly targeting IRF2
An increasing number of studies have suggested that microRNAs (miRNAs) are involved in the progress of many human cancers including osteosarcoma (OS). Especially, microRNA-18a-5p (miR-18a-5p) has been reported to associate with the occurrence, development and clinical outcomes of human cancers. Ther...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6096157/ https://www.ncbi.nlm.nih.gov/pubmed/30127908 http://dx.doi.org/10.3892/ol.2018.9032 |
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author | Lu, Chao Peng, Kan Guo, Hao Ren, Xiaoyu Hu, Shouye Cai, Yuanzhen Han, Yan Ma, Le Xu, Peng |
author_facet | Lu, Chao Peng, Kan Guo, Hao Ren, Xiaoyu Hu, Shouye Cai, Yuanzhen Han, Yan Ma, Le Xu, Peng |
author_sort | Lu, Chao |
collection | PubMed |
description | An increasing number of studies have suggested that microRNAs (miRNAs) are involved in the progress of many human cancers including osteosarcoma (OS). Especially, microRNA-18a-5p (miR-18a-5p) has been reported to associate with the occurrence, development and clinical outcomes of human cancers. Therefore, we investigated the functions of miR-18a-5p in OS. Reverse transcription-quantitative PCR (RT-qPCR) showed that miR-18a-5p was significantly upregulated in OS tissues and cell lines (MG-63 and Saos-2). The overexpression of miR-18a-5p was found to significantly promote cell migration and invasion in MG-63 cells via Transwell assay. Moreover, luciferase reporter assays indicated that interferon regulatory factor (IRF)2 was a direct target of miR-18a-5p. IRF2 was downregulated in MG-63 and Saos-2 cell lines. Furthermore, Transwell analysis showed that the knockout of IRF2 promoted cell migration and invasion in MG-63 cells. Carcinogenesis of miR-18a-5p was reversed by the overexpression of IRF2 in OS. In conclusion, miR-18a-5p promoted the invasion and migration of OS cells through inhibiting IRF2 expression. Thus, miR-18a-5p might act as a potential target for the diagnosis and treatment of OS in the future. |
format | Online Article Text |
id | pubmed-6096157 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-60961572018-08-20 miR-18a-5p promotes cell invasion and migration of osteosarcoma by directly targeting IRF2 Lu, Chao Peng, Kan Guo, Hao Ren, Xiaoyu Hu, Shouye Cai, Yuanzhen Han, Yan Ma, Le Xu, Peng Oncol Lett Articles An increasing number of studies have suggested that microRNAs (miRNAs) are involved in the progress of many human cancers including osteosarcoma (OS). Especially, microRNA-18a-5p (miR-18a-5p) has been reported to associate with the occurrence, development and clinical outcomes of human cancers. Therefore, we investigated the functions of miR-18a-5p in OS. Reverse transcription-quantitative PCR (RT-qPCR) showed that miR-18a-5p was significantly upregulated in OS tissues and cell lines (MG-63 and Saos-2). The overexpression of miR-18a-5p was found to significantly promote cell migration and invasion in MG-63 cells via Transwell assay. Moreover, luciferase reporter assays indicated that interferon regulatory factor (IRF)2 was a direct target of miR-18a-5p. IRF2 was downregulated in MG-63 and Saos-2 cell lines. Furthermore, Transwell analysis showed that the knockout of IRF2 promoted cell migration and invasion in MG-63 cells. Carcinogenesis of miR-18a-5p was reversed by the overexpression of IRF2 in OS. In conclusion, miR-18a-5p promoted the invasion and migration of OS cells through inhibiting IRF2 expression. Thus, miR-18a-5p might act as a potential target for the diagnosis and treatment of OS in the future. D.A. Spandidos 2018-09 2018-06-27 /pmc/articles/PMC6096157/ /pubmed/30127908 http://dx.doi.org/10.3892/ol.2018.9032 Text en Copyright: © Lu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Lu, Chao Peng, Kan Guo, Hao Ren, Xiaoyu Hu, Shouye Cai, Yuanzhen Han, Yan Ma, Le Xu, Peng miR-18a-5p promotes cell invasion and migration of osteosarcoma by directly targeting IRF2 |
title | miR-18a-5p promotes cell invasion and migration of osteosarcoma by directly targeting IRF2 |
title_full | miR-18a-5p promotes cell invasion and migration of osteosarcoma by directly targeting IRF2 |
title_fullStr | miR-18a-5p promotes cell invasion and migration of osteosarcoma by directly targeting IRF2 |
title_full_unstemmed | miR-18a-5p promotes cell invasion and migration of osteosarcoma by directly targeting IRF2 |
title_short | miR-18a-5p promotes cell invasion and migration of osteosarcoma by directly targeting IRF2 |
title_sort | mir-18a-5p promotes cell invasion and migration of osteosarcoma by directly targeting irf2 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6096157/ https://www.ncbi.nlm.nih.gov/pubmed/30127908 http://dx.doi.org/10.3892/ol.2018.9032 |
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