Cargando…
Identification of powdery mildew resistance QTL in strawberry (Fragaria × ananassa)
ABSTRACT: Key Message Powdery mildew resistance in two strawberry mapping populations is controlled by both stable and transient novel QTL of moderate effect. Some low transferability of QTL across wider germplasm was observed. ABSTRACT: The obligate biotrophic fungus Podosphaera aphanis is the caus...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6096635/ https://www.ncbi.nlm.nih.gov/pubmed/29971472 http://dx.doi.org/10.1007/s00122-018-3128-0 |
_version_ | 1783348140001198080 |
---|---|
author | Cockerton, Helen M. Vickerstaff, Robert J. Karlström, Amanda Wilson, Fiona Sobczyk, Maria He, Joe Q. Sargent, Daniel J. Passey, Andy J. McLeary, Kirsty J. Pakozdi, Katalin Harrison, Nicola Lumbreras-Martinez, Maria Antanaviciute, Laima Simpson, David W. Harrison, Richard J. |
author_facet | Cockerton, Helen M. Vickerstaff, Robert J. Karlström, Amanda Wilson, Fiona Sobczyk, Maria He, Joe Q. Sargent, Daniel J. Passey, Andy J. McLeary, Kirsty J. Pakozdi, Katalin Harrison, Nicola Lumbreras-Martinez, Maria Antanaviciute, Laima Simpson, David W. Harrison, Richard J. |
author_sort | Cockerton, Helen M. |
collection | PubMed |
description | ABSTRACT: Key Message Powdery mildew resistance in two strawberry mapping populations is controlled by both stable and transient novel QTL of moderate effect. Some low transferability of QTL across wider germplasm was observed. ABSTRACT: The obligate biotrophic fungus Podosphaera aphanis is the causative agent of powdery mildew on cultivated strawberry (Fragaria × ananassa). Genotypes from two bi-parental mapping populations ‘Emily’ × ‘Fenella’ and ‘Redgauntlet’ × ‘Hapil’ were phenotyped for powdery mildew disease severity in a series of field trials. Here, we report multiple QTL associated with resistance to powdery mildew, identified in ten phenotyping events conducted across different years and locations. Six QTL show a level of stable resistance across multiple phenotyping events; however, many other QTL were represented in a single phenotyping event and therefore must be considered transient. Subsequent screening of identified QTL across a validation set determined whether identified QTL remained closely linked to the associated resistance gene in the wider germplasm. Furthermore, a preliminary association analysis identified a novel conserved locus for further investigation. Our data suggest that resistance is highly complex and that multiple, primarily additive, sources of quantitative resistance to powdery mildew exist across strawberry germplasm. Utilisation of the reported markers in marker-assisted breeding or genomic selection would lead to improved powdery mildew-resistant strawberry cultivars, particularly where the studied parents, progeny and close pedigree material are included in breeding germplasm. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00122-018-3128-0) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6096635 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-60966352018-08-24 Identification of powdery mildew resistance QTL in strawberry (Fragaria × ananassa) Cockerton, Helen M. Vickerstaff, Robert J. Karlström, Amanda Wilson, Fiona Sobczyk, Maria He, Joe Q. Sargent, Daniel J. Passey, Andy J. McLeary, Kirsty J. Pakozdi, Katalin Harrison, Nicola Lumbreras-Martinez, Maria Antanaviciute, Laima Simpson, David W. Harrison, Richard J. Theor Appl Genet Original Article ABSTRACT: Key Message Powdery mildew resistance in two strawberry mapping populations is controlled by both stable and transient novel QTL of moderate effect. Some low transferability of QTL across wider germplasm was observed. ABSTRACT: The obligate biotrophic fungus Podosphaera aphanis is the causative agent of powdery mildew on cultivated strawberry (Fragaria × ananassa). Genotypes from two bi-parental mapping populations ‘Emily’ × ‘Fenella’ and ‘Redgauntlet’ × ‘Hapil’ were phenotyped for powdery mildew disease severity in a series of field trials. Here, we report multiple QTL associated with resistance to powdery mildew, identified in ten phenotyping events conducted across different years and locations. Six QTL show a level of stable resistance across multiple phenotyping events; however, many other QTL were represented in a single phenotyping event and therefore must be considered transient. Subsequent screening of identified QTL across a validation set determined whether identified QTL remained closely linked to the associated resistance gene in the wider germplasm. Furthermore, a preliminary association analysis identified a novel conserved locus for further investigation. Our data suggest that resistance is highly complex and that multiple, primarily additive, sources of quantitative resistance to powdery mildew exist across strawberry germplasm. Utilisation of the reported markers in marker-assisted breeding or genomic selection would lead to improved powdery mildew-resistant strawberry cultivars, particularly where the studied parents, progeny and close pedigree material are included in breeding germplasm. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00122-018-3128-0) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2018-07-03 2018 /pmc/articles/PMC6096635/ /pubmed/29971472 http://dx.doi.org/10.1007/s00122-018-3128-0 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Cockerton, Helen M. Vickerstaff, Robert J. Karlström, Amanda Wilson, Fiona Sobczyk, Maria He, Joe Q. Sargent, Daniel J. Passey, Andy J. McLeary, Kirsty J. Pakozdi, Katalin Harrison, Nicola Lumbreras-Martinez, Maria Antanaviciute, Laima Simpson, David W. Harrison, Richard J. Identification of powdery mildew resistance QTL in strawberry (Fragaria × ananassa) |
title | Identification of powdery mildew resistance QTL in strawberry (Fragaria × ananassa) |
title_full | Identification of powdery mildew resistance QTL in strawberry (Fragaria × ananassa) |
title_fullStr | Identification of powdery mildew resistance QTL in strawberry (Fragaria × ananassa) |
title_full_unstemmed | Identification of powdery mildew resistance QTL in strawberry (Fragaria × ananassa) |
title_short | Identification of powdery mildew resistance QTL in strawberry (Fragaria × ananassa) |
title_sort | identification of powdery mildew resistance qtl in strawberry (fragaria × ananassa) |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6096635/ https://www.ncbi.nlm.nih.gov/pubmed/29971472 http://dx.doi.org/10.1007/s00122-018-3128-0 |
work_keys_str_mv | AT cockertonhelenm identificationofpowderymildewresistanceqtlinstrawberryfragariaananassa AT vickerstaffrobertj identificationofpowderymildewresistanceqtlinstrawberryfragariaananassa AT karlstromamanda identificationofpowderymildewresistanceqtlinstrawberryfragariaananassa AT wilsonfiona identificationofpowderymildewresistanceqtlinstrawberryfragariaananassa AT sobczykmaria identificationofpowderymildewresistanceqtlinstrawberryfragariaananassa AT hejoeq identificationofpowderymildewresistanceqtlinstrawberryfragariaananassa AT sargentdanielj identificationofpowderymildewresistanceqtlinstrawberryfragariaananassa AT passeyandyj identificationofpowderymildewresistanceqtlinstrawberryfragariaananassa AT mclearykirstyj identificationofpowderymildewresistanceqtlinstrawberryfragariaananassa AT pakozdikatalin identificationofpowderymildewresistanceqtlinstrawberryfragariaananassa AT harrisonnicola identificationofpowderymildewresistanceqtlinstrawberryfragariaananassa AT lumbrerasmartinezmaria identificationofpowderymildewresistanceqtlinstrawberryfragariaananassa AT antanaviciutelaima identificationofpowderymildewresistanceqtlinstrawberryfragariaananassa AT simpsondavidw identificationofpowderymildewresistanceqtlinstrawberryfragariaananassa AT harrisonrichardj identificationofpowderymildewresistanceqtlinstrawberryfragariaananassa |