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Functional growth inhibition of influenza A and B viruses by liquid and powder components of leaves from the subtropical plant Melia azedarach L.
We evaluated the anti-influenza-virus effects of Melia components and discuss the utility of these components. The effects of leaf components of Melia azedarach L. on viruses were examined, and plaque inhibition tests were performed. The in vivo efficacy of M. azedarach L. was tested in a mouse mode...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Vienna
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6096724/ https://www.ncbi.nlm.nih.gov/pubmed/29633076 http://dx.doi.org/10.1007/s00705-018-3830-x |
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author | Nerome, Kuniaki Shimizu, Kazufumi Zukeran, Shiori Igarashi, Yasuhiro Kuroda, Kazumichi Sugita, Shigeo Shibata, Toshikatsu Ito, Yasuhiko Nerome, Reiko |
author_facet | Nerome, Kuniaki Shimizu, Kazufumi Zukeran, Shiori Igarashi, Yasuhiro Kuroda, Kazumichi Sugita, Shigeo Shibata, Toshikatsu Ito, Yasuhiko Nerome, Reiko |
author_sort | Nerome, Kuniaki |
collection | PubMed |
description | We evaluated the anti-influenza-virus effects of Melia components and discuss the utility of these components. The effects of leaf components of Melia azedarach L. on viruses were examined, and plaque inhibition tests were performed. The in vivo efficacy of M. azedarach L. was tested in a mouse model. Leaf components of Melia azedarach L. markedly inhibited the growth of various influenza viruses. In an initial screening, multiplication and haemagglutination (HA) activities of H1N1, H3N2, H5, and B influenza viruses were inactivated by the liquid extract of leaves of M. azedarach L. (MLE). Furthermore, plaque inhibition titres of H1N1, H3N2, and B influenza viruses treated with MLE ranged from 10(3.7) to 10(4.2). MLE possessed high plaque-inhibitory activity against pandemic avian H5N1, H7N9, and H9N2 vaccine candidate strains, with a plaque inhibition titre of more than 10(4.2). Notably, the buoyant density decreased from 1.175 to 1.137 g/cm(3), and spikeless particles appeared. We identified four anti-influenza virus substances: pheophorbide b, pheophorbide a, pyropheophorbide a, and pheophytin a. Photomorphogenesis inside the envelope may lead to removal of HA and neuraminidase spikes from viruses. Thus, MLE could efficiently remove floating influenza virus in the air space without toxicity. Consistent with this finding, intranasal administration of MLE in mice significantly decreased the occurrence of pneumonia. Additionally, leaf powder of Melia (MLP) inactivated influenza viruses and viruses in the intestines of chickens. MLE and MLP may have applications as novel, safe biological disinfectants for use in humans and poultry. |
format | Online Article Text |
id | pubmed-6096724 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer Vienna |
record_format | MEDLINE/PubMed |
spelling | pubmed-60967242018-08-24 Functional growth inhibition of influenza A and B viruses by liquid and powder components of leaves from the subtropical plant Melia azedarach L. Nerome, Kuniaki Shimizu, Kazufumi Zukeran, Shiori Igarashi, Yasuhiro Kuroda, Kazumichi Sugita, Shigeo Shibata, Toshikatsu Ito, Yasuhiko Nerome, Reiko Arch Virol Original Article We evaluated the anti-influenza-virus effects of Melia components and discuss the utility of these components. The effects of leaf components of Melia azedarach L. on viruses were examined, and plaque inhibition tests were performed. The in vivo efficacy of M. azedarach L. was tested in a mouse model. Leaf components of Melia azedarach L. markedly inhibited the growth of various influenza viruses. In an initial screening, multiplication and haemagglutination (HA) activities of H1N1, H3N2, H5, and B influenza viruses were inactivated by the liquid extract of leaves of M. azedarach L. (MLE). Furthermore, plaque inhibition titres of H1N1, H3N2, and B influenza viruses treated with MLE ranged from 10(3.7) to 10(4.2). MLE possessed high plaque-inhibitory activity against pandemic avian H5N1, H7N9, and H9N2 vaccine candidate strains, with a plaque inhibition titre of more than 10(4.2). Notably, the buoyant density decreased from 1.175 to 1.137 g/cm(3), and spikeless particles appeared. We identified four anti-influenza virus substances: pheophorbide b, pheophorbide a, pyropheophorbide a, and pheophytin a. Photomorphogenesis inside the envelope may lead to removal of HA and neuraminidase spikes from viruses. Thus, MLE could efficiently remove floating influenza virus in the air space without toxicity. Consistent with this finding, intranasal administration of MLE in mice significantly decreased the occurrence of pneumonia. Additionally, leaf powder of Melia (MLP) inactivated influenza viruses and viruses in the intestines of chickens. MLE and MLP may have applications as novel, safe biological disinfectants for use in humans and poultry. Springer Vienna 2018-04-09 2018 /pmc/articles/PMC6096724/ /pubmed/29633076 http://dx.doi.org/10.1007/s00705-018-3830-x Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Nerome, Kuniaki Shimizu, Kazufumi Zukeran, Shiori Igarashi, Yasuhiro Kuroda, Kazumichi Sugita, Shigeo Shibata, Toshikatsu Ito, Yasuhiko Nerome, Reiko Functional growth inhibition of influenza A and B viruses by liquid and powder components of leaves from the subtropical plant Melia azedarach L. |
title | Functional growth inhibition of influenza A and B viruses by liquid and powder components of leaves from the subtropical plant Melia azedarach L. |
title_full | Functional growth inhibition of influenza A and B viruses by liquid and powder components of leaves from the subtropical plant Melia azedarach L. |
title_fullStr | Functional growth inhibition of influenza A and B viruses by liquid and powder components of leaves from the subtropical plant Melia azedarach L. |
title_full_unstemmed | Functional growth inhibition of influenza A and B viruses by liquid and powder components of leaves from the subtropical plant Melia azedarach L. |
title_short | Functional growth inhibition of influenza A and B viruses by liquid and powder components of leaves from the subtropical plant Melia azedarach L. |
title_sort | functional growth inhibition of influenza a and b viruses by liquid and powder components of leaves from the subtropical plant melia azedarach l. |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6096724/ https://www.ncbi.nlm.nih.gov/pubmed/29633076 http://dx.doi.org/10.1007/s00705-018-3830-x |
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