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Nucleolus and chromatin
The nucleolus as site of ribosome biogenesis holds a pivotal role in cell metabolism. It is composed of ribosomal DNA (rDNA), which is present as tandem arrays located in nucleolus organizer regions (NORs). In interphase cells, rDNA can be found inside and adjacent to nucleoli and the location is in...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6096769/ https://www.ncbi.nlm.nih.gov/pubmed/30046888 http://dx.doi.org/10.1007/s00418-018-1696-3 |
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author | Schöfer, Christian Weipoltshammer, Klara |
author_facet | Schöfer, Christian Weipoltshammer, Klara |
author_sort | Schöfer, Christian |
collection | PubMed |
description | The nucleolus as site of ribosome biogenesis holds a pivotal role in cell metabolism. It is composed of ribosomal DNA (rDNA), which is present as tandem arrays located in nucleolus organizer regions (NORs). In interphase cells, rDNA can be found inside and adjacent to nucleoli and the location is indicative for transcriptional activity of ribosomal genes—inactive rDNA (outside) versus active one (inside). Moreover, the nucleolus itself acts as a spatial organizer of non-nucleolar chromatin. Microscopy-based approaches offer the possibility to explore the spatially distinct localization of the different DNA populations in relation to the nucleolar structure. Recent technical developments in microscopy and preparatory methods may further our understanding of the functional architecture of nucleoli. This review will attempt to summarize the current understanding of mammalian nucleolar chromatin organization as seen from a microscopist’s perspective. |
format | Online Article Text |
id | pubmed-6096769 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-60967692018-08-24 Nucleolus and chromatin Schöfer, Christian Weipoltshammer, Klara Histochem Cell Biol Review The nucleolus as site of ribosome biogenesis holds a pivotal role in cell metabolism. It is composed of ribosomal DNA (rDNA), which is present as tandem arrays located in nucleolus organizer regions (NORs). In interphase cells, rDNA can be found inside and adjacent to nucleoli and the location is indicative for transcriptional activity of ribosomal genes—inactive rDNA (outside) versus active one (inside). Moreover, the nucleolus itself acts as a spatial organizer of non-nucleolar chromatin. Microscopy-based approaches offer the possibility to explore the spatially distinct localization of the different DNA populations in relation to the nucleolar structure. Recent technical developments in microscopy and preparatory methods may further our understanding of the functional architecture of nucleoli. This review will attempt to summarize the current understanding of mammalian nucleolar chromatin organization as seen from a microscopist’s perspective. Springer Berlin Heidelberg 2018-07-25 2018 /pmc/articles/PMC6096769/ /pubmed/30046888 http://dx.doi.org/10.1007/s00418-018-1696-3 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Review Schöfer, Christian Weipoltshammer, Klara Nucleolus and chromatin |
title | Nucleolus and chromatin |
title_full | Nucleolus and chromatin |
title_fullStr | Nucleolus and chromatin |
title_full_unstemmed | Nucleolus and chromatin |
title_short | Nucleolus and chromatin |
title_sort | nucleolus and chromatin |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6096769/ https://www.ncbi.nlm.nih.gov/pubmed/30046888 http://dx.doi.org/10.1007/s00418-018-1696-3 |
work_keys_str_mv | AT schoferchristian nucleolusandchromatin AT weipoltshammerklara nucleolusandchromatin |