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Increased intracellular and extracellular oxidant production in phagocytes of rheumatic patients treated with biological therapy – whole blood quantification

Infectious complications, resulting from reduced activity of immune cells, are the most severe and common adverse effects of biological therapy. This study analyzed the effect of biological therapy on blood phagocytes, focusing on the formation of reactive oxygen species (ROS), an important factor i...

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Autores principales: JančInová, Viera, Nosáľ, Radomír, Payer, Juraj, Killinger, Zdenko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Slovak Toxicology Society SETOX 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6096858/
https://www.ncbi.nlm.nih.gov/pubmed/30123037
http://dx.doi.org/10.1515/intox-2017-0008
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author JančInová, Viera
Nosáľ, Radomír
Payer, Juraj
Killinger, Zdenko
author_facet JančInová, Viera
Nosáľ, Radomír
Payer, Juraj
Killinger, Zdenko
author_sort JančInová, Viera
collection PubMed
description Infectious complications, resulting from reduced activity of immune cells, are the most severe and common adverse effects of biological therapy. This study analyzed the effect of biological therapy on blood phagocytes, focusing on the formation of reactive oxygen species (ROS), an important factor in the defence against invading pathogens. Intra- and extracellular ROS production were recorded separately, on the basis of luminol and isoluminol chemiluminescence in patients treated with antibodies against tumor necrosis factor-α or against interleukin-6 receptor. In comparison to healthy donors or to rheumatic patients treated with classical immunosuppressive drugs, biological therapy increased ROS formation in both compartments. This indicates that the anti-microbial activity of blood phagocytes was not reduced by TNFα- or IL-6-neutralizing therapy, at least in terms of ROS. The method presented does not require blood fractionation, which could modify activity of phagocytes and cause loss of some subpopulations of these cells. The technique is simple, requires microliter volumes of blood and is thus well applicable to clinical studies.
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spelling pubmed-60968582018-08-17 Increased intracellular and extracellular oxidant production in phagocytes of rheumatic patients treated with biological therapy – whole blood quantification JančInová, Viera Nosáľ, Radomír Payer, Juraj Killinger, Zdenko Interdiscip Toxicol Original Article Infectious complications, resulting from reduced activity of immune cells, are the most severe and common adverse effects of biological therapy. This study analyzed the effect of biological therapy on blood phagocytes, focusing on the formation of reactive oxygen species (ROS), an important factor in the defence against invading pathogens. Intra- and extracellular ROS production were recorded separately, on the basis of luminol and isoluminol chemiluminescence in patients treated with antibodies against tumor necrosis factor-α or against interleukin-6 receptor. In comparison to healthy donors or to rheumatic patients treated with classical immunosuppressive drugs, biological therapy increased ROS formation in both compartments. This indicates that the anti-microbial activity of blood phagocytes was not reduced by TNFα- or IL-6-neutralizing therapy, at least in terms of ROS. The method presented does not require blood fractionation, which could modify activity of phagocytes and cause loss of some subpopulations of these cells. The technique is simple, requires microliter volumes of blood and is thus well applicable to clinical studies. Slovak Toxicology Society SETOX 2017-10 2018-02-14 /pmc/articles/PMC6096858/ /pubmed/30123037 http://dx.doi.org/10.1515/intox-2017-0008 Text en Copyright © 2017 SETOX & Institute of Experimental Pharmacology and Toxicology, SASc. https://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. (CC BY-NC-ND 4.0)
spellingShingle Original Article
JančInová, Viera
Nosáľ, Radomír
Payer, Juraj
Killinger, Zdenko
Increased intracellular and extracellular oxidant production in phagocytes of rheumatic patients treated with biological therapy – whole blood quantification
title Increased intracellular and extracellular oxidant production in phagocytes of rheumatic patients treated with biological therapy – whole blood quantification
title_full Increased intracellular and extracellular oxidant production in phagocytes of rheumatic patients treated with biological therapy – whole blood quantification
title_fullStr Increased intracellular and extracellular oxidant production in phagocytes of rheumatic patients treated with biological therapy – whole blood quantification
title_full_unstemmed Increased intracellular and extracellular oxidant production in phagocytes of rheumatic patients treated with biological therapy – whole blood quantification
title_short Increased intracellular and extracellular oxidant production in phagocytes of rheumatic patients treated with biological therapy – whole blood quantification
title_sort increased intracellular and extracellular oxidant production in phagocytes of rheumatic patients treated with biological therapy – whole blood quantification
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6096858/
https://www.ncbi.nlm.nih.gov/pubmed/30123037
http://dx.doi.org/10.1515/intox-2017-0008
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