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Transcriptome‐wide comparison of selenium hyperaccumulator and nonaccumulator Stanleya species provides new insight into key processes mediating the hyperaccumulation syndrome
To obtain better insight into the mechanisms of selenium hyperaccumulation in Stanleya pinnata, transcriptome‐wide differences in root and shoot gene expression levels were investigated in S. pinnata and related nonaccumulator Stanleya elata grown with or without 20 μm selenate. Genes predicted to b...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097121/ https://www.ncbi.nlm.nih.gov/pubmed/29412503 http://dx.doi.org/10.1111/pbi.12897 |
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author | Wang, Jiameng Cappa, Jennifer J. Harris, Jonathan P. Edger, Patrick P. Zhou, Wen Pires, J. Chris Adair, Michael Unruh, Sarah A. Simmons, Mark P. Schiavon, Michela Pilon‐Smits, Elizabeth A. H. |
author_facet | Wang, Jiameng Cappa, Jennifer J. Harris, Jonathan P. Edger, Patrick P. Zhou, Wen Pires, J. Chris Adair, Michael Unruh, Sarah A. Simmons, Mark P. Schiavon, Michela Pilon‐Smits, Elizabeth A. H. |
author_sort | Wang, Jiameng |
collection | PubMed |
description | To obtain better insight into the mechanisms of selenium hyperaccumulation in Stanleya pinnata, transcriptome‐wide differences in root and shoot gene expression levels were investigated in S. pinnata and related nonaccumulator Stanleya elata grown with or without 20 μm selenate. Genes predicted to be involved in sulphate/selenate transport and assimilation or in oxidative stress resistance (glutathione‐related genes and peroxidases) were among the most differentially expressed between species; many showed constitutively elevated expression in S. pinnata. A number of defence‐related genes predicted to mediate synthesis and signalling of defence hormones jasmonic acid (JA, reported to induce sulphur assimilatory and glutathione biosynthesis genes), salicylic acid (SA) and ethylene were also more expressed in S. pinnata than S. elata. Several upstream signalling genes that up‐regulate defence hormone synthesis showed higher expression in S. pinnata than S. elata and might trigger these selenium‐mediated defence responses. Thus, selenium hyperaccumulation and hypertolerance in S. pinnata may be mediated by constitutive, up‐regulated JA, SA and ethylene‐mediated defence systems, associated with elevated expression of genes involved in sulphate/selenate uptake and assimilation or in antioxidant activity. Genes pinpointed in this study may be targets of genetic engineering of plants that may be employed in biofortification or phytoremediation. |
format | Online Article Text |
id | pubmed-6097121 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60971212018-08-20 Transcriptome‐wide comparison of selenium hyperaccumulator and nonaccumulator Stanleya species provides new insight into key processes mediating the hyperaccumulation syndrome Wang, Jiameng Cappa, Jennifer J. Harris, Jonathan P. Edger, Patrick P. Zhou, Wen Pires, J. Chris Adair, Michael Unruh, Sarah A. Simmons, Mark P. Schiavon, Michela Pilon‐Smits, Elizabeth A. H. Plant Biotechnol J Research Articles To obtain better insight into the mechanisms of selenium hyperaccumulation in Stanleya pinnata, transcriptome‐wide differences in root and shoot gene expression levels were investigated in S. pinnata and related nonaccumulator Stanleya elata grown with or without 20 μm selenate. Genes predicted to be involved in sulphate/selenate transport and assimilation or in oxidative stress resistance (glutathione‐related genes and peroxidases) were among the most differentially expressed between species; many showed constitutively elevated expression in S. pinnata. A number of defence‐related genes predicted to mediate synthesis and signalling of defence hormones jasmonic acid (JA, reported to induce sulphur assimilatory and glutathione biosynthesis genes), salicylic acid (SA) and ethylene were also more expressed in S. pinnata than S. elata. Several upstream signalling genes that up‐regulate defence hormone synthesis showed higher expression in S. pinnata than S. elata and might trigger these selenium‐mediated defence responses. Thus, selenium hyperaccumulation and hypertolerance in S. pinnata may be mediated by constitutive, up‐regulated JA, SA and ethylene‐mediated defence systems, associated with elevated expression of genes involved in sulphate/selenate uptake and assimilation or in antioxidant activity. Genes pinpointed in this study may be targets of genetic engineering of plants that may be employed in biofortification or phytoremediation. John Wiley and Sons Inc. 2018-03-14 2018-09 /pmc/articles/PMC6097121/ /pubmed/29412503 http://dx.doi.org/10.1111/pbi.12897 Text en © 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Wang, Jiameng Cappa, Jennifer J. Harris, Jonathan P. Edger, Patrick P. Zhou, Wen Pires, J. Chris Adair, Michael Unruh, Sarah A. Simmons, Mark P. Schiavon, Michela Pilon‐Smits, Elizabeth A. H. Transcriptome‐wide comparison of selenium hyperaccumulator and nonaccumulator Stanleya species provides new insight into key processes mediating the hyperaccumulation syndrome |
title | Transcriptome‐wide comparison of selenium hyperaccumulator and nonaccumulator Stanleya species provides new insight into key processes mediating the hyperaccumulation syndrome |
title_full | Transcriptome‐wide comparison of selenium hyperaccumulator and nonaccumulator Stanleya species provides new insight into key processes mediating the hyperaccumulation syndrome |
title_fullStr | Transcriptome‐wide comparison of selenium hyperaccumulator and nonaccumulator Stanleya species provides new insight into key processes mediating the hyperaccumulation syndrome |
title_full_unstemmed | Transcriptome‐wide comparison of selenium hyperaccumulator and nonaccumulator Stanleya species provides new insight into key processes mediating the hyperaccumulation syndrome |
title_short | Transcriptome‐wide comparison of selenium hyperaccumulator and nonaccumulator Stanleya species provides new insight into key processes mediating the hyperaccumulation syndrome |
title_sort | transcriptome‐wide comparison of selenium hyperaccumulator and nonaccumulator stanleya species provides new insight into key processes mediating the hyperaccumulation syndrome |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097121/ https://www.ncbi.nlm.nih.gov/pubmed/29412503 http://dx.doi.org/10.1111/pbi.12897 |
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