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Alternative splicing of OsLG3b controls grain length and yield in japonica rice

Grain size, one of the important components determining grain yield in rice, is controlled by the multiple quantitative trait loci (QTLs). Intensive artificial selection for grain size during domestication is evidenced in modern cultivars compared to their wild relatives. Here, we report the molecul...

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Autores principales: Yu, Jianping, Miao, Jinli, Zhang, Zhanying, Xiong, Haiyan, Zhu, Xiaoyang, Sun, Xingming, Pan, Yinghua, Liang, Yuntao, Zhang, Qiang, Abdul Rehman, Rashid Muhammad, Li, Jinjie, Zhang, Hongliang, Li, Zichao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097128/
https://www.ncbi.nlm.nih.gov/pubmed/29479793
http://dx.doi.org/10.1111/pbi.12903
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author Yu, Jianping
Miao, Jinli
Zhang, Zhanying
Xiong, Haiyan
Zhu, Xiaoyang
Sun, Xingming
Pan, Yinghua
Liang, Yuntao
Zhang, Qiang
Abdul Rehman, Rashid Muhammad
Li, Jinjie
Zhang, Hongliang
Li, Zichao
author_facet Yu, Jianping
Miao, Jinli
Zhang, Zhanying
Xiong, Haiyan
Zhu, Xiaoyang
Sun, Xingming
Pan, Yinghua
Liang, Yuntao
Zhang, Qiang
Abdul Rehman, Rashid Muhammad
Li, Jinjie
Zhang, Hongliang
Li, Zichao
author_sort Yu, Jianping
collection PubMed
description Grain size, one of the important components determining grain yield in rice, is controlled by the multiple quantitative trait loci (QTLs). Intensive artificial selection for grain size during domestication is evidenced in modern cultivars compared to their wild relatives. Here, we report the molecular cloning and characterization of OsLG3b, a QTL for grain length in tropical japonica rice that encodes MADS‐box transcription factor 1 (OsMADS1). Six SNPs in the OsLG3b region led to alternative splicing, which were associated with grain length in an association analysis of candidate region. Quantitative PCR analysis indicated that OsLG3b expression was higher during the panicle and seed development stages. Analysis of haplotypes and introgression regions revealed that the long‐grain allele of OsLG3b might have arisen after domestication of tropical japonica and spread to subspecies indica or temperate japonica by natural crossing and artificial selection. OsLG3b is therefore a target of human selection for adaptation to tropical regions during domestication and/or improvement of rice. Phylogenetic analysis and pedigree records showed that OsLG3b had been employed by breeders, but the gene still has much breeding potential for increasing grain length in indica. These findings will not only aid efforts to elucidate the molecular basis of grain development and domestication, but also facilitate the genetic improvement of rice yield.
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spelling pubmed-60971282018-08-20 Alternative splicing of OsLG3b controls grain length and yield in japonica rice Yu, Jianping Miao, Jinli Zhang, Zhanying Xiong, Haiyan Zhu, Xiaoyang Sun, Xingming Pan, Yinghua Liang, Yuntao Zhang, Qiang Abdul Rehman, Rashid Muhammad Li, Jinjie Zhang, Hongliang Li, Zichao Plant Biotechnol J Research Articles Grain size, one of the important components determining grain yield in rice, is controlled by the multiple quantitative trait loci (QTLs). Intensive artificial selection for grain size during domestication is evidenced in modern cultivars compared to their wild relatives. Here, we report the molecular cloning and characterization of OsLG3b, a QTL for grain length in tropical japonica rice that encodes MADS‐box transcription factor 1 (OsMADS1). Six SNPs in the OsLG3b region led to alternative splicing, which were associated with grain length in an association analysis of candidate region. Quantitative PCR analysis indicated that OsLG3b expression was higher during the panicle and seed development stages. Analysis of haplotypes and introgression regions revealed that the long‐grain allele of OsLG3b might have arisen after domestication of tropical japonica and spread to subspecies indica or temperate japonica by natural crossing and artificial selection. OsLG3b is therefore a target of human selection for adaptation to tropical regions during domestication and/or improvement of rice. Phylogenetic analysis and pedigree records showed that OsLG3b had been employed by breeders, but the gene still has much breeding potential for increasing grain length in indica. These findings will not only aid efforts to elucidate the molecular basis of grain development and domestication, but also facilitate the genetic improvement of rice yield. John Wiley and Sons Inc. 2018-03-24 2018-09 /pmc/articles/PMC6097128/ /pubmed/29479793 http://dx.doi.org/10.1111/pbi.12903 Text en © 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Yu, Jianping
Miao, Jinli
Zhang, Zhanying
Xiong, Haiyan
Zhu, Xiaoyang
Sun, Xingming
Pan, Yinghua
Liang, Yuntao
Zhang, Qiang
Abdul Rehman, Rashid Muhammad
Li, Jinjie
Zhang, Hongliang
Li, Zichao
Alternative splicing of OsLG3b controls grain length and yield in japonica rice
title Alternative splicing of OsLG3b controls grain length and yield in japonica rice
title_full Alternative splicing of OsLG3b controls grain length and yield in japonica rice
title_fullStr Alternative splicing of OsLG3b controls grain length and yield in japonica rice
title_full_unstemmed Alternative splicing of OsLG3b controls grain length and yield in japonica rice
title_short Alternative splicing of OsLG3b controls grain length and yield in japonica rice
title_sort alternative splicing of oslg3b controls grain length and yield in japonica rice
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097128/
https://www.ncbi.nlm.nih.gov/pubmed/29479793
http://dx.doi.org/10.1111/pbi.12903
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