Cargando…
Alternative splicing of OsLG3b controls grain length and yield in japonica rice
Grain size, one of the important components determining grain yield in rice, is controlled by the multiple quantitative trait loci (QTLs). Intensive artificial selection for grain size during domestication is evidenced in modern cultivars compared to their wild relatives. Here, we report the molecul...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097128/ https://www.ncbi.nlm.nih.gov/pubmed/29479793 http://dx.doi.org/10.1111/pbi.12903 |
_version_ | 1783348243714801664 |
---|---|
author | Yu, Jianping Miao, Jinli Zhang, Zhanying Xiong, Haiyan Zhu, Xiaoyang Sun, Xingming Pan, Yinghua Liang, Yuntao Zhang, Qiang Abdul Rehman, Rashid Muhammad Li, Jinjie Zhang, Hongliang Li, Zichao |
author_facet | Yu, Jianping Miao, Jinli Zhang, Zhanying Xiong, Haiyan Zhu, Xiaoyang Sun, Xingming Pan, Yinghua Liang, Yuntao Zhang, Qiang Abdul Rehman, Rashid Muhammad Li, Jinjie Zhang, Hongliang Li, Zichao |
author_sort | Yu, Jianping |
collection | PubMed |
description | Grain size, one of the important components determining grain yield in rice, is controlled by the multiple quantitative trait loci (QTLs). Intensive artificial selection for grain size during domestication is evidenced in modern cultivars compared to their wild relatives. Here, we report the molecular cloning and characterization of OsLG3b, a QTL for grain length in tropical japonica rice that encodes MADS‐box transcription factor 1 (OsMADS1). Six SNPs in the OsLG3b region led to alternative splicing, which were associated with grain length in an association analysis of candidate region. Quantitative PCR analysis indicated that OsLG3b expression was higher during the panicle and seed development stages. Analysis of haplotypes and introgression regions revealed that the long‐grain allele of OsLG3b might have arisen after domestication of tropical japonica and spread to subspecies indica or temperate japonica by natural crossing and artificial selection. OsLG3b is therefore a target of human selection for adaptation to tropical regions during domestication and/or improvement of rice. Phylogenetic analysis and pedigree records showed that OsLG3b had been employed by breeders, but the gene still has much breeding potential for increasing grain length in indica. These findings will not only aid efforts to elucidate the molecular basis of grain development and domestication, but also facilitate the genetic improvement of rice yield. |
format | Online Article Text |
id | pubmed-6097128 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60971282018-08-20 Alternative splicing of OsLG3b controls grain length and yield in japonica rice Yu, Jianping Miao, Jinli Zhang, Zhanying Xiong, Haiyan Zhu, Xiaoyang Sun, Xingming Pan, Yinghua Liang, Yuntao Zhang, Qiang Abdul Rehman, Rashid Muhammad Li, Jinjie Zhang, Hongliang Li, Zichao Plant Biotechnol J Research Articles Grain size, one of the important components determining grain yield in rice, is controlled by the multiple quantitative trait loci (QTLs). Intensive artificial selection for grain size during domestication is evidenced in modern cultivars compared to their wild relatives. Here, we report the molecular cloning and characterization of OsLG3b, a QTL for grain length in tropical japonica rice that encodes MADS‐box transcription factor 1 (OsMADS1). Six SNPs in the OsLG3b region led to alternative splicing, which were associated with grain length in an association analysis of candidate region. Quantitative PCR analysis indicated that OsLG3b expression was higher during the panicle and seed development stages. Analysis of haplotypes and introgression regions revealed that the long‐grain allele of OsLG3b might have arisen after domestication of tropical japonica and spread to subspecies indica or temperate japonica by natural crossing and artificial selection. OsLG3b is therefore a target of human selection for adaptation to tropical regions during domestication and/or improvement of rice. Phylogenetic analysis and pedigree records showed that OsLG3b had been employed by breeders, but the gene still has much breeding potential for increasing grain length in indica. These findings will not only aid efforts to elucidate the molecular basis of grain development and domestication, but also facilitate the genetic improvement of rice yield. John Wiley and Sons Inc. 2018-03-24 2018-09 /pmc/articles/PMC6097128/ /pubmed/29479793 http://dx.doi.org/10.1111/pbi.12903 Text en © 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Yu, Jianping Miao, Jinli Zhang, Zhanying Xiong, Haiyan Zhu, Xiaoyang Sun, Xingming Pan, Yinghua Liang, Yuntao Zhang, Qiang Abdul Rehman, Rashid Muhammad Li, Jinjie Zhang, Hongliang Li, Zichao Alternative splicing of OsLG3b controls grain length and yield in japonica rice |
title | Alternative splicing of OsLG3b controls grain length and yield in japonica rice |
title_full | Alternative splicing of OsLG3b controls grain length and yield in japonica rice |
title_fullStr | Alternative splicing of OsLG3b controls grain length and yield in japonica rice |
title_full_unstemmed | Alternative splicing of OsLG3b controls grain length and yield in japonica rice |
title_short | Alternative splicing of OsLG3b controls grain length and yield in japonica rice |
title_sort | alternative splicing of oslg3b controls grain length and yield in japonica rice |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097128/ https://www.ncbi.nlm.nih.gov/pubmed/29479793 http://dx.doi.org/10.1111/pbi.12903 |
work_keys_str_mv | AT yujianping alternativesplicingofoslg3bcontrolsgrainlengthandyieldinjaponicarice AT miaojinli alternativesplicingofoslg3bcontrolsgrainlengthandyieldinjaponicarice AT zhangzhanying alternativesplicingofoslg3bcontrolsgrainlengthandyieldinjaponicarice AT xionghaiyan alternativesplicingofoslg3bcontrolsgrainlengthandyieldinjaponicarice AT zhuxiaoyang alternativesplicingofoslg3bcontrolsgrainlengthandyieldinjaponicarice AT sunxingming alternativesplicingofoslg3bcontrolsgrainlengthandyieldinjaponicarice AT panyinghua alternativesplicingofoslg3bcontrolsgrainlengthandyieldinjaponicarice AT liangyuntao alternativesplicingofoslg3bcontrolsgrainlengthandyieldinjaponicarice AT zhangqiang alternativesplicingofoslg3bcontrolsgrainlengthandyieldinjaponicarice AT abdulrehmanrashidmuhammad alternativesplicingofoslg3bcontrolsgrainlengthandyieldinjaponicarice AT lijinjie alternativesplicingofoslg3bcontrolsgrainlengthandyieldinjaponicarice AT zhanghongliang alternativesplicingofoslg3bcontrolsgrainlengthandyieldinjaponicarice AT lizichao alternativesplicingofoslg3bcontrolsgrainlengthandyieldinjaponicarice |