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Electrical Stimulation Prevents Preferential Skeletal Muscle Myosin Loss in Steroid-Denervation Rats
Severe muscle weakness concomitant with preferential depletion of myosin has been observed in several pathological conditions. Here, we used the steroid-denervation (S-D) rat model, which shows dramatic decrease in myosin content and force production, to test whether electrical stimulation (ES) trea...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097132/ https://www.ncbi.nlm.nih.gov/pubmed/30147660 http://dx.doi.org/10.3389/fphys.2018.01111 |
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author | Yamada, Takashi Himori, Koichi Tatebayashi, Daisuke Yamada, Ryotaro Ashida, Yuki Imai, Tomihiro Akatsuka, Masayuki Masuda, Yoshiki Kanzaki, Keita Watanabe, Daiki Wada, Masanobu Westerblad, Håkan Lanner, Johanna T. |
author_facet | Yamada, Takashi Himori, Koichi Tatebayashi, Daisuke Yamada, Ryotaro Ashida, Yuki Imai, Tomihiro Akatsuka, Masayuki Masuda, Yoshiki Kanzaki, Keita Watanabe, Daiki Wada, Masanobu Westerblad, Håkan Lanner, Johanna T. |
author_sort | Yamada, Takashi |
collection | PubMed |
description | Severe muscle weakness concomitant with preferential depletion of myosin has been observed in several pathological conditions. Here, we used the steroid-denervation (S-D) rat model, which shows dramatic decrease in myosin content and force production, to test whether electrical stimulation (ES) treatment can prevent these deleterious changes. S-D was induced by cutting the sciatic nerve and subsequent daily injection of dexamethasone for 7 days. For ES treatment, plantarflexor muscles were electrically stimulated to produce four sets of five isometric contractions each day. Plantarflexor in situ isometric torque, muscle weight, skinned muscle fiber force, and protein and mRNA expression were measured after the intervention period. ES treatment partly prevented the S-D-induced decreases in plantarflexor in situ isometric torque and muscle weight. ES treatment fully prevented S-D-induced decreases in skinned fiber force and ratio of myosin heavy chain (MyHC) to actin, as well as increases in the reactive oxygen/nitrogen species-generating enzymes NADPH oxidase (NOX) 2 and 4, phosphorylation of p38 MAPK, mRNA expression of the muscle-specific ubiquitin ligases muscle ring finger-1 (MuRF-1) and atrogin-1, and autolyzed active calpain-1. Thus, ES treatment is an effective way to prevent muscle impairments associated with loss of myosin. |
format | Online Article Text |
id | pubmed-6097132 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60971322018-08-24 Electrical Stimulation Prevents Preferential Skeletal Muscle Myosin Loss in Steroid-Denervation Rats Yamada, Takashi Himori, Koichi Tatebayashi, Daisuke Yamada, Ryotaro Ashida, Yuki Imai, Tomihiro Akatsuka, Masayuki Masuda, Yoshiki Kanzaki, Keita Watanabe, Daiki Wada, Masanobu Westerblad, Håkan Lanner, Johanna T. Front Physiol Physiology Severe muscle weakness concomitant with preferential depletion of myosin has been observed in several pathological conditions. Here, we used the steroid-denervation (S-D) rat model, which shows dramatic decrease in myosin content and force production, to test whether electrical stimulation (ES) treatment can prevent these deleterious changes. S-D was induced by cutting the sciatic nerve and subsequent daily injection of dexamethasone for 7 days. For ES treatment, plantarflexor muscles were electrically stimulated to produce four sets of five isometric contractions each day. Plantarflexor in situ isometric torque, muscle weight, skinned muscle fiber force, and protein and mRNA expression were measured after the intervention period. ES treatment partly prevented the S-D-induced decreases in plantarflexor in situ isometric torque and muscle weight. ES treatment fully prevented S-D-induced decreases in skinned fiber force and ratio of myosin heavy chain (MyHC) to actin, as well as increases in the reactive oxygen/nitrogen species-generating enzymes NADPH oxidase (NOX) 2 and 4, phosphorylation of p38 MAPK, mRNA expression of the muscle-specific ubiquitin ligases muscle ring finger-1 (MuRF-1) and atrogin-1, and autolyzed active calpain-1. Thus, ES treatment is an effective way to prevent muscle impairments associated with loss of myosin. Frontiers Media S.A. 2018-08-10 /pmc/articles/PMC6097132/ /pubmed/30147660 http://dx.doi.org/10.3389/fphys.2018.01111 Text en Copyright © 2018 Yamada, Himori, Tatebayashi, Yamada, Ashida, Imai, Akatsuka, Masuda, Kanzaki, Watanabe, Wada, Westerblad and Lanner. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Yamada, Takashi Himori, Koichi Tatebayashi, Daisuke Yamada, Ryotaro Ashida, Yuki Imai, Tomihiro Akatsuka, Masayuki Masuda, Yoshiki Kanzaki, Keita Watanabe, Daiki Wada, Masanobu Westerblad, Håkan Lanner, Johanna T. Electrical Stimulation Prevents Preferential Skeletal Muscle Myosin Loss in Steroid-Denervation Rats |
title | Electrical Stimulation Prevents Preferential Skeletal Muscle Myosin Loss in Steroid-Denervation Rats |
title_full | Electrical Stimulation Prevents Preferential Skeletal Muscle Myosin Loss in Steroid-Denervation Rats |
title_fullStr | Electrical Stimulation Prevents Preferential Skeletal Muscle Myosin Loss in Steroid-Denervation Rats |
title_full_unstemmed | Electrical Stimulation Prevents Preferential Skeletal Muscle Myosin Loss in Steroid-Denervation Rats |
title_short | Electrical Stimulation Prevents Preferential Skeletal Muscle Myosin Loss in Steroid-Denervation Rats |
title_sort | electrical stimulation prevents preferential skeletal muscle myosin loss in steroid-denervation rats |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097132/ https://www.ncbi.nlm.nih.gov/pubmed/30147660 http://dx.doi.org/10.3389/fphys.2018.01111 |
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