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Toward Light‐Regulated Living Biomaterials
Living materials are an emergent material class, infused with the productive, adaptive, and regenerative properties of living organisms. Property regulation in living materials requires encoding responsive units in the living components to allow external manipulation of their function. Here, an opto...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097140/ https://www.ncbi.nlm.nih.gov/pubmed/30128245 http://dx.doi.org/10.1002/advs.201800383 |
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author | Sankaran, Shrikrishnan Zhao, Shifang Muth, Christina Paez, Julieta del Campo, Aránzazu |
author_facet | Sankaran, Shrikrishnan Zhao, Shifang Muth, Christina Paez, Julieta del Campo, Aránzazu |
author_sort | Sankaran, Shrikrishnan |
collection | PubMed |
description | Living materials are an emergent material class, infused with the productive, adaptive, and regenerative properties of living organisms. Property regulation in living materials requires encoding responsive units in the living components to allow external manipulation of their function. Here, an optoregulated Escherichia coli (E. coli)‐based living biomaterial that can be externally addressed using light to interact with mammalian cells is demonstrated. This is achieved by using a photoactivatable inducer of gene expression and bacterial surface display technology to present an integrin‐specific miniprotein on the outer membrane of an endotoxin‐free E. coli strain. Hydrogel surfaces functionalized with the bacteria can expose cell adhesive molecules upon in situ light‐activation, and trigger cell adhesion. Surface immobilized bacteria are able to deliver a fluorescent protein to the mammalian cells with which they are interacting, indicating the potential of such a bacterial material to deliver molecules to cells in a targeted manner. |
format | Online Article Text |
id | pubmed-6097140 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60971402018-08-20 Toward Light‐Regulated Living Biomaterials Sankaran, Shrikrishnan Zhao, Shifang Muth, Christina Paez, Julieta del Campo, Aránzazu Adv Sci (Weinh) Communications Living materials are an emergent material class, infused with the productive, adaptive, and regenerative properties of living organisms. Property regulation in living materials requires encoding responsive units in the living components to allow external manipulation of their function. Here, an optoregulated Escherichia coli (E. coli)‐based living biomaterial that can be externally addressed using light to interact with mammalian cells is demonstrated. This is achieved by using a photoactivatable inducer of gene expression and bacterial surface display technology to present an integrin‐specific miniprotein on the outer membrane of an endotoxin‐free E. coli strain. Hydrogel surfaces functionalized with the bacteria can expose cell adhesive molecules upon in situ light‐activation, and trigger cell adhesion. Surface immobilized bacteria are able to deliver a fluorescent protein to the mammalian cells with which they are interacting, indicating the potential of such a bacterial material to deliver molecules to cells in a targeted manner. John Wiley and Sons Inc. 2018-06-29 /pmc/articles/PMC6097140/ /pubmed/30128245 http://dx.doi.org/10.1002/advs.201800383 Text en © 2018 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Communications Sankaran, Shrikrishnan Zhao, Shifang Muth, Christina Paez, Julieta del Campo, Aránzazu Toward Light‐Regulated Living Biomaterials |
title | Toward Light‐Regulated Living Biomaterials |
title_full | Toward Light‐Regulated Living Biomaterials |
title_fullStr | Toward Light‐Regulated Living Biomaterials |
title_full_unstemmed | Toward Light‐Regulated Living Biomaterials |
title_short | Toward Light‐Regulated Living Biomaterials |
title_sort | toward light‐regulated living biomaterials |
topic | Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097140/ https://www.ncbi.nlm.nih.gov/pubmed/30128245 http://dx.doi.org/10.1002/advs.201800383 |
work_keys_str_mv | AT sankaranshrikrishnan towardlightregulatedlivingbiomaterials AT zhaoshifang towardlightregulatedlivingbiomaterials AT muthchristina towardlightregulatedlivingbiomaterials AT paezjulieta towardlightregulatedlivingbiomaterials AT delcampoaranzazu towardlightregulatedlivingbiomaterials |