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Immunohistochemical study of alpha-smooth muscle actin in odontogenic cysts and tumors

CONTEXT: Myofibroblasts (MFs) are fibroblasts with smooth muscle-like features characterized by the presence of a contractile apparatus. Alpha-smooth muscle actin (α-SMA) is the actin isoform that predominates within vascular smooth muscle cells and plays an important role in fibrogenesis. MFs are m...

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Autores principales: Annegowda, Vidya Mallipattana, Devi, HS Uma, Rao, Kavita, Smitha, T, Sheethal, HS, Smitha, A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097363/
https://www.ncbi.nlm.nih.gov/pubmed/30158770
http://dx.doi.org/10.4103/jomfp.JOMFP_31_18
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author Annegowda, Vidya Mallipattana
Devi, HS Uma
Rao, Kavita
Smitha, T
Sheethal, HS
Smitha, A
author_facet Annegowda, Vidya Mallipattana
Devi, HS Uma
Rao, Kavita
Smitha, T
Sheethal, HS
Smitha, A
author_sort Annegowda, Vidya Mallipattana
collection PubMed
description CONTEXT: Myofibroblasts (MFs) are fibroblasts with smooth muscle-like features characterized by the presence of a contractile apparatus. Alpha-smooth muscle actin (α-SMA) is the actin isoform that predominates within vascular smooth muscle cells and plays an important role in fibrogenesis. MFs are metabolically and morphologically distinctive fibroblasts expressing α-SMA, and their activation plays a key role in development of the fibrotic response. AIMS AND OBJECTIVES: The aim of this study is to demonstrate the frequency, distribution and expression of α-SMA-positive MFs in odontogenic keratocyst (OKC), dentigerous cyst (DC) and ameloblastoma and correlate it to their aggressive biological behavior. SETTINGS AND DESIGN: A retrospective study of 45 diagnosed cases, which includes 15 cases of OKC, 15 cases of DC and 15 cases of ameloblastoma, was undertaken to demonstrate expression of α-SMA retrieved from archives of our department. MATERIALS AND METHODS: α-SMA mouse anti-human antibody and horseradish peroxidase detection system were used in this study. STATISTICAL ANALYSIS: Descriptive statistical analysis and ANOVA test were used for statistical analysis. RESULTS: The difference in mean α-SMA count was found to be statistically significant between ameloblastoma and DC group (P < 0.001) as well as OKC and DC group (P < 0.001). No significant difference is observed between ameloblastoma and OKC group (P > 0.05). Results showed that mean number of stromal MFs in OKC and ameloblastoma were significantly higher than DC. CONCLUSION: The present study has shown that the mean number of MFs was higher in OKC and ameloblastoma, while the mean number of MFs in DC was quite low and significantly different from that of ameloblastoma and OKC.
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spelling pubmed-60973632018-08-29 Immunohistochemical study of alpha-smooth muscle actin in odontogenic cysts and tumors Annegowda, Vidya Mallipattana Devi, HS Uma Rao, Kavita Smitha, T Sheethal, HS Smitha, A J Oral Maxillofac Pathol Original Article CONTEXT: Myofibroblasts (MFs) are fibroblasts with smooth muscle-like features characterized by the presence of a contractile apparatus. Alpha-smooth muscle actin (α-SMA) is the actin isoform that predominates within vascular smooth muscle cells and plays an important role in fibrogenesis. MFs are metabolically and morphologically distinctive fibroblasts expressing α-SMA, and their activation plays a key role in development of the fibrotic response. AIMS AND OBJECTIVES: The aim of this study is to demonstrate the frequency, distribution and expression of α-SMA-positive MFs in odontogenic keratocyst (OKC), dentigerous cyst (DC) and ameloblastoma and correlate it to their aggressive biological behavior. SETTINGS AND DESIGN: A retrospective study of 45 diagnosed cases, which includes 15 cases of OKC, 15 cases of DC and 15 cases of ameloblastoma, was undertaken to demonstrate expression of α-SMA retrieved from archives of our department. MATERIALS AND METHODS: α-SMA mouse anti-human antibody and horseradish peroxidase detection system were used in this study. STATISTICAL ANALYSIS: Descriptive statistical analysis and ANOVA test were used for statistical analysis. RESULTS: The difference in mean α-SMA count was found to be statistically significant between ameloblastoma and DC group (P < 0.001) as well as OKC and DC group (P < 0.001). No significant difference is observed between ameloblastoma and OKC group (P > 0.05). Results showed that mean number of stromal MFs in OKC and ameloblastoma were significantly higher than DC. CONCLUSION: The present study has shown that the mean number of MFs was higher in OKC and ameloblastoma, while the mean number of MFs in DC was quite low and significantly different from that of ameloblastoma and OKC. Medknow Publications & Media Pvt Ltd 2018 /pmc/articles/PMC6097363/ /pubmed/30158770 http://dx.doi.org/10.4103/jomfp.JOMFP_31_18 Text en Copyright: © 2018 Journal of Oral and Maxillofacial Pathology http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Annegowda, Vidya Mallipattana
Devi, HS Uma
Rao, Kavita
Smitha, T
Sheethal, HS
Smitha, A
Immunohistochemical study of alpha-smooth muscle actin in odontogenic cysts and tumors
title Immunohistochemical study of alpha-smooth muscle actin in odontogenic cysts and tumors
title_full Immunohistochemical study of alpha-smooth muscle actin in odontogenic cysts and tumors
title_fullStr Immunohistochemical study of alpha-smooth muscle actin in odontogenic cysts and tumors
title_full_unstemmed Immunohistochemical study of alpha-smooth muscle actin in odontogenic cysts and tumors
title_short Immunohistochemical study of alpha-smooth muscle actin in odontogenic cysts and tumors
title_sort immunohistochemical study of alpha-smooth muscle actin in odontogenic cysts and tumors
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097363/
https://www.ncbi.nlm.nih.gov/pubmed/30158770
http://dx.doi.org/10.4103/jomfp.JOMFP_31_18
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