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Modifications and optimization of manual methods for polymerase chain reaction and 16S rRNA gene sequencing quality community DNA extraction from goat rumen digesta

BACKGROUND AND AIM: A critical prerequisite for studying rumen microbial community by high throughput molecular biology methods is good quality community DNA. Current methods of extraction use kits designed for samples from the different origin for rumen. This puts stress on the development of a rel...

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Detalles Bibliográficos
Autores principales:	Aphale, Durgadevi, Kulkarni, Aarohi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Veterinary World 2018
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097561/ https://www.ncbi.nlm.nih.gov/pubmed/30147271 http://dx.doi.org/10.14202/vetworld.2018.990-1000

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