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Genome mapping of seed-borne allergens and immunoresponsive proteins in wheat
Wheat is an important staple grain for humankind globally because of its end-use quality and nutritional properties and its adaptability to diverse climates. For a small proportion of the population, specific wheat proteins can trigger adverse immune responses and clinical manifestations such as cel...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097586/ https://www.ncbi.nlm.nih.gov/pubmed/30128352 http://dx.doi.org/10.1126/sciadv.aar8602 |
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author | Juhász, Angéla Belova, Tatiana Florides, Chris G. Maulis, Csaba Fischer, Iris Gell, Gyöngyvér Birinyi, Zsófia Ong, Jamie Keeble-Gagnère, Gabriel Maharajan, Amudha Ma, Wujun Gibson, Peter Jia, Jizeng Lang, Daniel Mayer, Klaus F. X. Spannagl, Manuel Tye-Din, Jason A. Appels, Rudi Olsen, Odd-Arne |
author_facet | Juhász, Angéla Belova, Tatiana Florides, Chris G. Maulis, Csaba Fischer, Iris Gell, Gyöngyvér Birinyi, Zsófia Ong, Jamie Keeble-Gagnère, Gabriel Maharajan, Amudha Ma, Wujun Gibson, Peter Jia, Jizeng Lang, Daniel Mayer, Klaus F. X. Spannagl, Manuel Tye-Din, Jason A. Appels, Rudi Olsen, Odd-Arne |
author_sort | Juhász, Angéla |
collection | PubMed |
description | Wheat is an important staple grain for humankind globally because of its end-use quality and nutritional properties and its adaptability to diverse climates. For a small proportion of the population, specific wheat proteins can trigger adverse immune responses and clinical manifestations such as celiac disease, wheat allergy, baker’s asthma, and wheat-dependent exercise-induced anaphylaxis (WDEIA). Establishing the content and distribution of the immunostimulatory regions in wheat has been hampered by the complexity of the wheat genome and the lack of complete genome sequence information. We provide novel insights into the wheat grain proteins based on a comprehensive analysis and annotation of the wheat prolamin Pfam clan grain proteins and other non-prolamin allergens implicated in these disorders using the new International Wheat Genome Sequencing Consortium bread wheat reference genome sequence, RefSeq v1.0. Celiac disease and WDEIA genes are primarily expressed in the starchy endosperm and show wide variation in protein- and transcript-level expression in response to temperature stress. Nonspecific lipid transfer proteins and α-amylase trypsin inhibitor gene families, implicated in baker’s asthma, are primarily expressed in the aleurone layer and transfer cells of grains and are more sensitive to cold temperature. The study establishes a new reference map for immunostimulatory wheat proteins and provides a fresh basis for selecting wheat lines and developing diagnostics for products with more favorable consumer attributes. |
format | Online Article Text |
id | pubmed-6097586 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-60975862018-08-20 Genome mapping of seed-borne allergens and immunoresponsive proteins in wheat Juhász, Angéla Belova, Tatiana Florides, Chris G. Maulis, Csaba Fischer, Iris Gell, Gyöngyvér Birinyi, Zsófia Ong, Jamie Keeble-Gagnère, Gabriel Maharajan, Amudha Ma, Wujun Gibson, Peter Jia, Jizeng Lang, Daniel Mayer, Klaus F. X. Spannagl, Manuel Tye-Din, Jason A. Appels, Rudi Olsen, Odd-Arne Sci Adv Research Articles Wheat is an important staple grain for humankind globally because of its end-use quality and nutritional properties and its adaptability to diverse climates. For a small proportion of the population, specific wheat proteins can trigger adverse immune responses and clinical manifestations such as celiac disease, wheat allergy, baker’s asthma, and wheat-dependent exercise-induced anaphylaxis (WDEIA). Establishing the content and distribution of the immunostimulatory regions in wheat has been hampered by the complexity of the wheat genome and the lack of complete genome sequence information. We provide novel insights into the wheat grain proteins based on a comprehensive analysis and annotation of the wheat prolamin Pfam clan grain proteins and other non-prolamin allergens implicated in these disorders using the new International Wheat Genome Sequencing Consortium bread wheat reference genome sequence, RefSeq v1.0. Celiac disease and WDEIA genes are primarily expressed in the starchy endosperm and show wide variation in protein- and transcript-level expression in response to temperature stress. Nonspecific lipid transfer proteins and α-amylase trypsin inhibitor gene families, implicated in baker’s asthma, are primarily expressed in the aleurone layer and transfer cells of grains and are more sensitive to cold temperature. The study establishes a new reference map for immunostimulatory wheat proteins and provides a fresh basis for selecting wheat lines and developing diagnostics for products with more favorable consumer attributes. American Association for the Advancement of Science 2018-08-17 /pmc/articles/PMC6097586/ /pubmed/30128352 http://dx.doi.org/10.1126/sciadv.aar8602 Text en Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Research Articles Juhász, Angéla Belova, Tatiana Florides, Chris G. Maulis, Csaba Fischer, Iris Gell, Gyöngyvér Birinyi, Zsófia Ong, Jamie Keeble-Gagnère, Gabriel Maharajan, Amudha Ma, Wujun Gibson, Peter Jia, Jizeng Lang, Daniel Mayer, Klaus F. X. Spannagl, Manuel Tye-Din, Jason A. Appels, Rudi Olsen, Odd-Arne Genome mapping of seed-borne allergens and immunoresponsive proteins in wheat |
title | Genome mapping of seed-borne allergens and immunoresponsive proteins in wheat |
title_full | Genome mapping of seed-borne allergens and immunoresponsive proteins in wheat |
title_fullStr | Genome mapping of seed-borne allergens and immunoresponsive proteins in wheat |
title_full_unstemmed | Genome mapping of seed-borne allergens and immunoresponsive proteins in wheat |
title_short | Genome mapping of seed-borne allergens and immunoresponsive proteins in wheat |
title_sort | genome mapping of seed-borne allergens and immunoresponsive proteins in wheat |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097586/ https://www.ncbi.nlm.nih.gov/pubmed/30128352 http://dx.doi.org/10.1126/sciadv.aar8602 |
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