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Identification and removal of sequencing artifacts produced by mispriming during reverse transcription in multiple RNA-seq technologies
The quality of RNA sequencing data relies on specific priming by the primer used for reverse transcription (RT-primer). Nonspecific annealing of the RT-primer to the RNA template can generate reads with incorrect cDNA ends and can cause misinterpretation of data (RT mispriming). This kind of artifac...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Cold Spring Harbor Laboratory Press
2018
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097653/ https://www.ncbi.nlm.nih.gov/pubmed/29950518 http://dx.doi.org/10.1261/rna.066217.118 |