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Simple Detection of Cochliobolus Fungal Pathogens in Maize

Northern corn leaf spot and southern corn leaf blight caused by Cochliobolus carbonum (anamorph, Bipolaris zeicola) and Cochliobolus heterostrophus (anamorph, Bipolaris maydis), respectively, are common maize diseases in Korea. Accurate detection of plant pathogens is necessary for effective disease...

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Autores principales: Kang, In Jeong, Shim, Hyeong Kwon, Roh, Jae Hwan, Heu, Sunggi, Shin, Dong Bum
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Plant Pathology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097825/
https://www.ncbi.nlm.nih.gov/pubmed/30140186
http://dx.doi.org/10.5423/PPJ.FT.10.2017.0209
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author Kang, In Jeong
Shim, Hyeong Kwon
Roh, Jae Hwan
Heu, Sunggi
Shin, Dong Bum
author_facet Kang, In Jeong
Shim, Hyeong Kwon
Roh, Jae Hwan
Heu, Sunggi
Shin, Dong Bum
author_sort Kang, In Jeong
collection PubMed
description Northern corn leaf spot and southern corn leaf blight caused by Cochliobolus carbonum (anamorph, Bipolaris zeicola) and Cochliobolus heterostrophus (anamorph, Bipolaris maydis), respectively, are common maize diseases in Korea. Accurate detection of plant pathogens is necessary for effective disease management. Based on the polyketide synthase gene (PKS) of Cochliobolus carbonum and the nonribosomal peptide synthetase gene (NRPS) of Cochliobolus heterostrophus, primer pairs were designed for PCR to simultaneously detect the two fungal pathogens and were specific and sensitive enough to be used for duplex PCR analysis. This duplex PCR-based method was found to be effective for diagnosing simultaneous infections from the two Cochliobolus species that display similar morphological and mycological characteristics. With this method, it is possible to prevent infections in maize by detecting infected seeds or maize and discarding them. Besides saving time and effort, early diagnosis can help to prevent infections, establish comprehensive management systems, and secure healthy seeds.
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spelling pubmed-60978252018-08-23 Simple Detection of Cochliobolus Fungal Pathogens in Maize Kang, In Jeong Shim, Hyeong Kwon Roh, Jae Hwan Heu, Sunggi Shin, Dong Bum Plant Pathol J Research Article Northern corn leaf spot and southern corn leaf blight caused by Cochliobolus carbonum (anamorph, Bipolaris zeicola) and Cochliobolus heterostrophus (anamorph, Bipolaris maydis), respectively, are common maize diseases in Korea. Accurate detection of plant pathogens is necessary for effective disease management. Based on the polyketide synthase gene (PKS) of Cochliobolus carbonum and the nonribosomal peptide synthetase gene (NRPS) of Cochliobolus heterostrophus, primer pairs were designed for PCR to simultaneously detect the two fungal pathogens and were specific and sensitive enough to be used for duplex PCR analysis. This duplex PCR-based method was found to be effective for diagnosing simultaneous infections from the two Cochliobolus species that display similar morphological and mycological characteristics. With this method, it is possible to prevent infections in maize by detecting infected seeds or maize and discarding them. Besides saving time and effort, early diagnosis can help to prevent infections, establish comprehensive management systems, and secure healthy seeds. Korean Society of Plant Pathology 2018-08 2018-08-01 /pmc/articles/PMC6097825/ /pubmed/30140186 http://dx.doi.org/10.5423/PPJ.FT.10.2017.0209 Text en © The Korean Society of Plant Pathology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kang, In Jeong
Shim, Hyeong Kwon
Roh, Jae Hwan
Heu, Sunggi
Shin, Dong Bum
Simple Detection of Cochliobolus Fungal Pathogens in Maize
title Simple Detection of Cochliobolus Fungal Pathogens in Maize
title_full Simple Detection of Cochliobolus Fungal Pathogens in Maize
title_fullStr Simple Detection of Cochliobolus Fungal Pathogens in Maize
title_full_unstemmed Simple Detection of Cochliobolus Fungal Pathogens in Maize
title_short Simple Detection of Cochliobolus Fungal Pathogens in Maize
title_sort simple detection of cochliobolus fungal pathogens in maize
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097825/
https://www.ncbi.nlm.nih.gov/pubmed/30140186
http://dx.doi.org/10.5423/PPJ.FT.10.2017.0209
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