Cargando…
Simple Detection of Cochliobolus Fungal Pathogens in Maize
Northern corn leaf spot and southern corn leaf blight caused by Cochliobolus carbonum (anamorph, Bipolaris zeicola) and Cochliobolus heterostrophus (anamorph, Bipolaris maydis), respectively, are common maize diseases in Korea. Accurate detection of plant pathogens is necessary for effective disease...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society of Plant Pathology
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097825/ https://www.ncbi.nlm.nih.gov/pubmed/30140186 http://dx.doi.org/10.5423/PPJ.FT.10.2017.0209 |
_version_ | 1783348372907753472 |
---|---|
author | Kang, In Jeong Shim, Hyeong Kwon Roh, Jae Hwan Heu, Sunggi Shin, Dong Bum |
author_facet | Kang, In Jeong Shim, Hyeong Kwon Roh, Jae Hwan Heu, Sunggi Shin, Dong Bum |
author_sort | Kang, In Jeong |
collection | PubMed |
description | Northern corn leaf spot and southern corn leaf blight caused by Cochliobolus carbonum (anamorph, Bipolaris zeicola) and Cochliobolus heterostrophus (anamorph, Bipolaris maydis), respectively, are common maize diseases in Korea. Accurate detection of plant pathogens is necessary for effective disease management. Based on the polyketide synthase gene (PKS) of Cochliobolus carbonum and the nonribosomal peptide synthetase gene (NRPS) of Cochliobolus heterostrophus, primer pairs were designed for PCR to simultaneously detect the two fungal pathogens and were specific and sensitive enough to be used for duplex PCR analysis. This duplex PCR-based method was found to be effective for diagnosing simultaneous infections from the two Cochliobolus species that display similar morphological and mycological characteristics. With this method, it is possible to prevent infections in maize by detecting infected seeds or maize and discarding them. Besides saving time and effort, early diagnosis can help to prevent infections, establish comprehensive management systems, and secure healthy seeds. |
format | Online Article Text |
id | pubmed-6097825 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Korean Society of Plant Pathology |
record_format | MEDLINE/PubMed |
spelling | pubmed-60978252018-08-23 Simple Detection of Cochliobolus Fungal Pathogens in Maize Kang, In Jeong Shim, Hyeong Kwon Roh, Jae Hwan Heu, Sunggi Shin, Dong Bum Plant Pathol J Research Article Northern corn leaf spot and southern corn leaf blight caused by Cochliobolus carbonum (anamorph, Bipolaris zeicola) and Cochliobolus heterostrophus (anamorph, Bipolaris maydis), respectively, are common maize diseases in Korea. Accurate detection of plant pathogens is necessary for effective disease management. Based on the polyketide synthase gene (PKS) of Cochliobolus carbonum and the nonribosomal peptide synthetase gene (NRPS) of Cochliobolus heterostrophus, primer pairs were designed for PCR to simultaneously detect the two fungal pathogens and were specific and sensitive enough to be used for duplex PCR analysis. This duplex PCR-based method was found to be effective for diagnosing simultaneous infections from the two Cochliobolus species that display similar morphological and mycological characteristics. With this method, it is possible to prevent infections in maize by detecting infected seeds or maize and discarding them. Besides saving time and effort, early diagnosis can help to prevent infections, establish comprehensive management systems, and secure healthy seeds. Korean Society of Plant Pathology 2018-08 2018-08-01 /pmc/articles/PMC6097825/ /pubmed/30140186 http://dx.doi.org/10.5423/PPJ.FT.10.2017.0209 Text en © The Korean Society of Plant Pathology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kang, In Jeong Shim, Hyeong Kwon Roh, Jae Hwan Heu, Sunggi Shin, Dong Bum Simple Detection of Cochliobolus Fungal Pathogens in Maize |
title | Simple Detection of Cochliobolus Fungal Pathogens in Maize |
title_full | Simple Detection of Cochliobolus Fungal Pathogens in Maize |
title_fullStr | Simple Detection of Cochliobolus Fungal Pathogens in Maize |
title_full_unstemmed | Simple Detection of Cochliobolus Fungal Pathogens in Maize |
title_short | Simple Detection of Cochliobolus Fungal Pathogens in Maize |
title_sort | simple detection of cochliobolus fungal pathogens in maize |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097825/ https://www.ncbi.nlm.nih.gov/pubmed/30140186 http://dx.doi.org/10.5423/PPJ.FT.10.2017.0209 |
work_keys_str_mv | AT kanginjeong simpledetectionofcochliobolusfungalpathogensinmaize AT shimhyeongkwon simpledetectionofcochliobolusfungalpathogensinmaize AT rohjaehwan simpledetectionofcochliobolusfungalpathogensinmaize AT heusunggi simpledetectionofcochliobolusfungalpathogensinmaize AT shindongbum simpledetectionofcochliobolusfungalpathogensinmaize |