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3D Bioprinted Human Skeletal Muscle Constructs for Muscle Function Restoration
A bioengineered skeletal muscle tissue as an alternative for autologous tissue flaps, which mimics the structural and functional characteristics of the native tissue, is needed for reconstructive surgery. Rapid progress in the cell-based tissue engineering principle has enabled in vitro creation of...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6098064/ https://www.ncbi.nlm.nih.gov/pubmed/30120282 http://dx.doi.org/10.1038/s41598-018-29968-5 |
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author | Kim, Ji Hyun Seol, Young-Joon Ko, In Kap Kang, Hyun-Wook Lee, Young Koo Yoo, James J. Atala, Anthony Lee, Sang Jin |
author_facet | Kim, Ji Hyun Seol, Young-Joon Ko, In Kap Kang, Hyun-Wook Lee, Young Koo Yoo, James J. Atala, Anthony Lee, Sang Jin |
author_sort | Kim, Ji Hyun |
collection | PubMed |
description | A bioengineered skeletal muscle tissue as an alternative for autologous tissue flaps, which mimics the structural and functional characteristics of the native tissue, is needed for reconstructive surgery. Rapid progress in the cell-based tissue engineering principle has enabled in vitro creation of cellularized muscle-like constructs; however, the current fabrication methods are still limited to build a three-dimensional (3D) muscle construct with a highly viable, organized cellular structure with the potential for a future human trial. Here, we applied 3D bioprinting strategy to fabricate an implantable, bioengineered skeletal muscle tissue composed of human primary muscle progenitor cells (hMPCs). The bioprinted skeletal muscle tissue showed a highly organized multi-layered muscle bundle made by viable, densely packed, and aligned myofiber-like structures. Our in vivo study presented that the bioprinted muscle constructs reached 82% of functional recovery in a rodent model of tibialis anterior (TA) muscle defect at 8 weeks of post-implantation. In addition, histological and immunohistological examinations indicated that the bioprinted muscle constructs were well integrated with host vascular and neural networks. We demonstrated the potential of the use of the 3D bioprinted skeletal muscle with a spatially organized structure that can reconstruct the extensive muscle defects. |
format | Online Article Text |
id | pubmed-6098064 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-60980642018-08-23 3D Bioprinted Human Skeletal Muscle Constructs for Muscle Function Restoration Kim, Ji Hyun Seol, Young-Joon Ko, In Kap Kang, Hyun-Wook Lee, Young Koo Yoo, James J. Atala, Anthony Lee, Sang Jin Sci Rep Article A bioengineered skeletal muscle tissue as an alternative for autologous tissue flaps, which mimics the structural and functional characteristics of the native tissue, is needed for reconstructive surgery. Rapid progress in the cell-based tissue engineering principle has enabled in vitro creation of cellularized muscle-like constructs; however, the current fabrication methods are still limited to build a three-dimensional (3D) muscle construct with a highly viable, organized cellular structure with the potential for a future human trial. Here, we applied 3D bioprinting strategy to fabricate an implantable, bioengineered skeletal muscle tissue composed of human primary muscle progenitor cells (hMPCs). The bioprinted skeletal muscle tissue showed a highly organized multi-layered muscle bundle made by viable, densely packed, and aligned myofiber-like structures. Our in vivo study presented that the bioprinted muscle constructs reached 82% of functional recovery in a rodent model of tibialis anterior (TA) muscle defect at 8 weeks of post-implantation. In addition, histological and immunohistological examinations indicated that the bioprinted muscle constructs were well integrated with host vascular and neural networks. We demonstrated the potential of the use of the 3D bioprinted skeletal muscle with a spatially organized structure that can reconstruct the extensive muscle defects. Nature Publishing Group UK 2018-08-17 /pmc/articles/PMC6098064/ /pubmed/30120282 http://dx.doi.org/10.1038/s41598-018-29968-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kim, Ji Hyun Seol, Young-Joon Ko, In Kap Kang, Hyun-Wook Lee, Young Koo Yoo, James J. Atala, Anthony Lee, Sang Jin 3D Bioprinted Human Skeletal Muscle Constructs for Muscle Function Restoration |
title | 3D Bioprinted Human Skeletal Muscle Constructs for Muscle Function Restoration |
title_full | 3D Bioprinted Human Skeletal Muscle Constructs for Muscle Function Restoration |
title_fullStr | 3D Bioprinted Human Skeletal Muscle Constructs for Muscle Function Restoration |
title_full_unstemmed | 3D Bioprinted Human Skeletal Muscle Constructs for Muscle Function Restoration |
title_short | 3D Bioprinted Human Skeletal Muscle Constructs for Muscle Function Restoration |
title_sort | 3d bioprinted human skeletal muscle constructs for muscle function restoration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6098064/ https://www.ncbi.nlm.nih.gov/pubmed/30120282 http://dx.doi.org/10.1038/s41598-018-29968-5 |
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