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Multi-photon near-infrared emission saturation nanoscopy using upconversion nanoparticles
Multiphoton fluorescence microscopy (MPM), using near infrared excitation light, provides increased penetration depth, decreased detection background, and reduced phototoxicity. Using stimulated emission depletion (STED) approach, MPM can bypass the diffraction limitation, but it requires both spati...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6098146/ https://www.ncbi.nlm.nih.gov/pubmed/30120242 http://dx.doi.org/10.1038/s41467-018-05842-w |
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author | Chen, Chaohao Wang, Fan Wen, Shihui Su, Qian Peter Wu, Mike C. L. Liu, Yongtao Wang, Baoming Li, Du Shan, Xuchen Kianinia, Mehran Aharonovich, Igor Toth, Milos Jackson, Shaun P. Xi, Peng Jin, Dayong |
author_facet | Chen, Chaohao Wang, Fan Wen, Shihui Su, Qian Peter Wu, Mike C. L. Liu, Yongtao Wang, Baoming Li, Du Shan, Xuchen Kianinia, Mehran Aharonovich, Igor Toth, Milos Jackson, Shaun P. Xi, Peng Jin, Dayong |
author_sort | Chen, Chaohao |
collection | PubMed |
description | Multiphoton fluorescence microscopy (MPM), using near infrared excitation light, provides increased penetration depth, decreased detection background, and reduced phototoxicity. Using stimulated emission depletion (STED) approach, MPM can bypass the diffraction limitation, but it requires both spatial alignment and temporal synchronization of high power (femtosecond) lasers, which is limited by the inefficiency of the probes. Here, we report that upconversion nanoparticles (UCNPs) can unlock a new mode of near-infrared emission saturation (NIRES) nanoscopy for deep tissue super-resolution imaging with excitation intensity several orders of magnitude lower than that required by conventional MPM dyes. Using a doughnut beam excitation from a 980 nm diode laser and detecting at 800 nm, we achieve a resolution of sub 50 nm, 1/20th of the excitation wavelength, in imaging of single UCNP through 93 μm thick liver tissue. This method offers a simple solution for deep tissue super resolution imaging and single molecule tracking. |
format | Online Article Text |
id | pubmed-6098146 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-60981462018-08-20 Multi-photon near-infrared emission saturation nanoscopy using upconversion nanoparticles Chen, Chaohao Wang, Fan Wen, Shihui Su, Qian Peter Wu, Mike C. L. Liu, Yongtao Wang, Baoming Li, Du Shan, Xuchen Kianinia, Mehran Aharonovich, Igor Toth, Milos Jackson, Shaun P. Xi, Peng Jin, Dayong Nat Commun Article Multiphoton fluorescence microscopy (MPM), using near infrared excitation light, provides increased penetration depth, decreased detection background, and reduced phototoxicity. Using stimulated emission depletion (STED) approach, MPM can bypass the diffraction limitation, but it requires both spatial alignment and temporal synchronization of high power (femtosecond) lasers, which is limited by the inefficiency of the probes. Here, we report that upconversion nanoparticles (UCNPs) can unlock a new mode of near-infrared emission saturation (NIRES) nanoscopy for deep tissue super-resolution imaging with excitation intensity several orders of magnitude lower than that required by conventional MPM dyes. Using a doughnut beam excitation from a 980 nm diode laser and detecting at 800 nm, we achieve a resolution of sub 50 nm, 1/20th of the excitation wavelength, in imaging of single UCNP through 93 μm thick liver tissue. This method offers a simple solution for deep tissue super resolution imaging and single molecule tracking. Nature Publishing Group UK 2018-08-17 /pmc/articles/PMC6098146/ /pubmed/30120242 http://dx.doi.org/10.1038/s41467-018-05842-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Chen, Chaohao Wang, Fan Wen, Shihui Su, Qian Peter Wu, Mike C. L. Liu, Yongtao Wang, Baoming Li, Du Shan, Xuchen Kianinia, Mehran Aharonovich, Igor Toth, Milos Jackson, Shaun P. Xi, Peng Jin, Dayong Multi-photon near-infrared emission saturation nanoscopy using upconversion nanoparticles |
title | Multi-photon near-infrared emission saturation nanoscopy using upconversion nanoparticles |
title_full | Multi-photon near-infrared emission saturation nanoscopy using upconversion nanoparticles |
title_fullStr | Multi-photon near-infrared emission saturation nanoscopy using upconversion nanoparticles |
title_full_unstemmed | Multi-photon near-infrared emission saturation nanoscopy using upconversion nanoparticles |
title_short | Multi-photon near-infrared emission saturation nanoscopy using upconversion nanoparticles |
title_sort | multi-photon near-infrared emission saturation nanoscopy using upconversion nanoparticles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6098146/ https://www.ncbi.nlm.nih.gov/pubmed/30120242 http://dx.doi.org/10.1038/s41467-018-05842-w |
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