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The Arp8 and Arp4 module acts as a DNA sensor controlling INO80 chromatin remodeling

Nuclear actin and actin-related proteins (Arps) are key components of chromatin remodeling and modifying complexes. Although Arps are essential for the functions of chromatin remodelers, their specific roles and mechanisms are unclear. Here we define the nucleosome binding interfaces and functions o...

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Autores principales: Brahma, Sandipan, Ngubo, Mzwanele, Paul, Somnath, Udugama, Maheshi, Bartholomew, Blaine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6098158/
https://www.ncbi.nlm.nih.gov/pubmed/30120252
http://dx.doi.org/10.1038/s41467-018-05710-7
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author Brahma, Sandipan
Ngubo, Mzwanele
Paul, Somnath
Udugama, Maheshi
Bartholomew, Blaine
author_facet Brahma, Sandipan
Ngubo, Mzwanele
Paul, Somnath
Udugama, Maheshi
Bartholomew, Blaine
author_sort Brahma, Sandipan
collection PubMed
description Nuclear actin and actin-related proteins (Arps) are key components of chromatin remodeling and modifying complexes. Although Arps are essential for the functions of chromatin remodelers, their specific roles and mechanisms are unclear. Here we define the nucleosome binding interfaces and functions of the evolutionarily conserved Arps in the yeast INO80 chromatin remodeling complex. We show that the N-terminus of Arp8, C-terminus of Arp4 and the HSA domain of Ino80 bind extranucleosomal DNA 37–51 base pairs from the edge of nucleosomes and function as a DNA-length sensor that regulates nucleosome sliding by INO80. Disruption of Arp8 and Arp4 binding to DNA uncouples ATP hydrolysis from nucleosome mobilization by disengaging Arp5 from the acidic patch on histone H2A-H2B and the Ino80-ATPase domain from the Super-helical Location (SHL) -6 of nucleosomes. Our data suggest a functional interplay between INO80’s Arp8-Arp4-actin and Arp5 modules in sensing the DNA length separating nucleosomes and regulating nucleosome positioning.
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spelling pubmed-60981582018-08-20 The Arp8 and Arp4 module acts as a DNA sensor controlling INO80 chromatin remodeling Brahma, Sandipan Ngubo, Mzwanele Paul, Somnath Udugama, Maheshi Bartholomew, Blaine Nat Commun Article Nuclear actin and actin-related proteins (Arps) are key components of chromatin remodeling and modifying complexes. Although Arps are essential for the functions of chromatin remodelers, their specific roles and mechanisms are unclear. Here we define the nucleosome binding interfaces and functions of the evolutionarily conserved Arps in the yeast INO80 chromatin remodeling complex. We show that the N-terminus of Arp8, C-terminus of Arp4 and the HSA domain of Ino80 bind extranucleosomal DNA 37–51 base pairs from the edge of nucleosomes and function as a DNA-length sensor that regulates nucleosome sliding by INO80. Disruption of Arp8 and Arp4 binding to DNA uncouples ATP hydrolysis from nucleosome mobilization by disengaging Arp5 from the acidic patch on histone H2A-H2B and the Ino80-ATPase domain from the Super-helical Location (SHL) -6 of nucleosomes. Our data suggest a functional interplay between INO80’s Arp8-Arp4-actin and Arp5 modules in sensing the DNA length separating nucleosomes and regulating nucleosome positioning. Nature Publishing Group UK 2018-08-17 /pmc/articles/PMC6098158/ /pubmed/30120252 http://dx.doi.org/10.1038/s41467-018-05710-7 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Brahma, Sandipan
Ngubo, Mzwanele
Paul, Somnath
Udugama, Maheshi
Bartholomew, Blaine
The Arp8 and Arp4 module acts as a DNA sensor controlling INO80 chromatin remodeling
title The Arp8 and Arp4 module acts as a DNA sensor controlling INO80 chromatin remodeling
title_full The Arp8 and Arp4 module acts as a DNA sensor controlling INO80 chromatin remodeling
title_fullStr The Arp8 and Arp4 module acts as a DNA sensor controlling INO80 chromatin remodeling
title_full_unstemmed The Arp8 and Arp4 module acts as a DNA sensor controlling INO80 chromatin remodeling
title_short The Arp8 and Arp4 module acts as a DNA sensor controlling INO80 chromatin remodeling
title_sort arp8 and arp4 module acts as a dna sensor controlling ino80 chromatin remodeling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6098158/
https://www.ncbi.nlm.nih.gov/pubmed/30120252
http://dx.doi.org/10.1038/s41467-018-05710-7
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